High-throughput decontamination technology for autologous hematopoietic stem-cell transplantation

2014 Fiscal Year Final Research Report

• Project/Area Number: 25560190
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: The University of Tokyo
• Principal Investigator: GODA Keisuke 東京大学, 理学(系)研究科(研究院), 教授 (70518696)
• Co-Investigator(Kenkyū-buntansha): YAMANE Daisuke 東京工業大学, 精密工学研究所, 助教 (70634096)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: フローサイトメトリー / マイクロ流体工学 / レーザー / 白血病 / 自家造血幹細胞移植

Outline of Final Research Achievements

We developed an optofluidic system for high-throughput screening of a large heterogeneous population of cells based on their morphological and biochemical features. This system is based on an integration of an ultrafast optical imager with a multi-color fluorescence analyzer for simultaneous image- and fluorescence-based evaluation of every single cell in a large population with a high throughput of 10,000 cells/s. This system is expected to be useful for high-throughput identification and removal of cancer cells that contaminate grafts in autologous hematopoietic stem-cell transplantation. This work has been absorbed into the ImPACT program in which a far more advanced system is currently under development.

Free Research Field

光科学