2014 Fiscal Year Final Research Report
Development of Efficient Expansion for Human Pluripotent Stem Cells
| Project/Area Number |
25560195
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Biomedical engineering/Biomaterial science and engineering
|
|---|
| Research Institution | Kyoto University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2013-04-01 – 2015-03-31
|
| Keywords | 幹細胞 / ES細胞 / iPS細胞 / 大量培養 |
|---|
| Outline of Final Research Achievements |
Human pluripotent stem cells (hPSCs) including human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) is thought to provide renewable source for a wide range of applications in regenerative medicine, and as tools for human disease modeling and drug discovery. For these purposes, large numbers of cells with high quality are essential. Recently, we show that biological substrate, recombinant E8 fragments of laminin isoforms (LM-E8s) sustain long-term self-renewal of hPSCs in defined, zeno-freee media with dissociated single-cell passing. However, biological materials are generally expensive to manufacture, have limited scalability and may have batch-to-batch variability. In this study, we developed our culture system to show similar performance using LM-E8s to efficient expansion of hPSCs under defined, xeno-free conditions using non-biological synthetic substrates.
|
| Free Research Field |
幹細胞
|
