2014 Fiscal Year Final Research Report
Challenge to the accurate detection of minimal drug resistant cells
Project/Area Number |
25670184
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Experimental pathology
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Research Institution | Hokkaido University |
Principal Investigator |
OHBA Yusuke 北海道大学, 医学(系)研究科(研究院), 教授 (30333503)
|
Project Period (FY) |
2013-04-01 – 2015-03-31
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Keywords | バイオイメージング / 腫瘍 / 薬剤耐性 / フローサイトメーター |
Outline of Final Research Achievements |
In Philadelphia chromosome-positive leukemia, growth and survival of leukemia cells solely depend on constitutive tyrosine kinase activity of new fusion gene product BCR-ABL formed by chromosome reciprocal translocation between chromosomes 9 and 22. Therefore, the treatment for this disease has been radically improved by the introduction of the specific tyrosine kinase inhibitor of BCR-ABL. However, it is desired to develop new technologies that enable the detection of a minimal number of drug-resistant cells because substantial patients experience drug resistance regardless of the presence or absence of past and current treatment. We have previously developed a novel technique to detect relatively small number of drug resistant cells. Here, we challenged to centuplicate the detection ability by the improvement of the FRET biosensor, the detection method and the analytical method. As a result, we obtained 162-fold improvement throughout this study.
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Free Research Field |
実験病理学
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