Phospho-signaling pathway dependent on an ATM kinase activated by oxidative stress

2014 Fiscal Year Final Research Report

• Project/Area Number: 25871242
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General medical chemistry; Risk sciences of radiation and chemicals
• Research Institution: 独立行政法人医薬基盤研究所
• Principal Investigator: HASHIGUCHI Kazunari 独立行政法人医薬基盤研究所, 創薬基盤研究部, 特任研究員 (80400414)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: ATM / キナーゼ / リン酸化 / シグナル伝達 / 活性酸素 / 酸化ストレス

Outline of Final Research Achievements

Although an ATM has been supposed to be a kinase that is activated by DNA damage response, recent work revealed that it is also activated by oxidative stress thorough distinct regulation. In this study, I attempt to figure out ATM-dependent phospho-signaling pathway in these two activation systems. For detection of substrates phosphorylated by ATM, several anti-phospho ATM substrate antibodies are used in Western blot analyses. After giving two kinds of cellular stresses, wild-type cells show different substrate pattern even if ATM-deficient cells has same, indicating that detected substrates are specific to ATM and different cellular signals give different substrates which are in ATM-dependent pathway. Further analysis, such as phospho-proteome analysis of ATM substrates, will give valuable information in phosphor-signaling pathways response to various cellular stresses.

Free Research Field

分子生物学