Application to establish the orthodontic treatment of pro-angiogenic technology using periodontal ligament-derived vascular endothelial precursor cells

2014 Fiscal Year Final Research Report

• Project/Area Number: 25893220
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Iwate Medical University
• Principal Investigator: YOSHIDA Mariko 岩手医科大学, 歯学部, 研究員 (80711217)
• Project Period (FY): 2013-08-30 – 2015-03-31
• Keywords: 歯根膜 / 血管内皮前駆細胞 / 血管新生 / 血管内皮細胞 / 血管周皮細胞 / 細胞分化

Outline of Final Research Achievements

We examined how contractile and suppressive forces affect the ability of periodontal ligament (PDL)-derived endothelial precursor cells (EPCs) to differentiate into endothelial cells (ECs) or pericytes. We found that contractile force against PDL-derived EPCs downregulated EC marker Tie-2 expression, but upregulated pericyte marker α-SMA expression. On the contrary, suppressive force against PDL-derived EPCs upregulated EC marker Tie-2 expression, but downregulated pericyte marker α-SMA expression. Thus, we succeeded to establish in vitro culture system by which we are able to evaluate how suppressive mechanical stress affects the multi-differentiation ability of these cells. We are trying to identify key genes to positively and negatively regulate differentiation of PDL-derived EPCs into ECs and perycytes.

Free Research Field

口腔生化学