2017 Fiscal Year Final Research Report
Investigation of horizontal transmission among N2O emission-associated genes and N2O emission suppressing chemical agents
| Project/Area Number |
26252058
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental agriculture(including landscape science)
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
内田 義崇 北海道大学, 農学研究院, 准教授 (70705251)
村井 勇太 北海道大学, 先端生命科学研究院, 助教 (20707038)
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| Co-Investigator(Renkei-kenkyūsha) |
内田 義崇 北海道大学, 大学院農学研究院, 助教 (70705251)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Keywords | N2O放出ホットスポット / N2O放出細菌 / 不完全脱窒細菌 / 脱窒関連遺伝子 / 遺伝子水平伝播 / N2O放出抑制因子 / 微生物機能性分化因子 / 無機窒素代謝 |
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| Outline of Final Research Achievements |
Active N2O emitting microorganisms were searched for, at the soils from reclaimed tropical peatland in Malaysia, Andisol corn farmland in Hokkaido, and palsa mire-degrading boreal peat in Finland Lapland, all of which are known as active N2O emission hotspots. Resulting active N2O emitting bacteria were compared their physiological and biological traits. Burkholderia spp. isolated as a hyper-active N2O emitter from tropical peat soils s were missing nosZ genes, while 4 strains out of 10 active N2O emitting Pseudomonas spp. rather harbored weakly functional nosZ gene. In boreal peatland, particularly degrading palsa bogs, Rhizobium sp. and Serratia sp. mixed colonies had abilities to release N2O from NO2-.
In investigation of N2O production-repressing chemicals, Paraquat and allylisothiocyanate showed relatively strong N2O-emission inhibitory effect. For further screening, several differentiation-inducing factors toward bacteria were isolated and identified.
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| Free Research Field |
微生物生態化学
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