Development for an ultrasensitive and non-labeled detection method of non-fluorescent molecules in liquid solutions

2017 Fiscal Year Final Research Report

• Project/Area Number: 26288068
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Analytical chemistry
• Research Institution: Kyushu University
• Principal Investigator: Harata Akira 九州大学, 総合理工学研究院, 教授 (90222231)
• Co-Investigator(Renkei-kenkyūsha): ISODA Miki 九州大学, 大学院総合理工学府, 技術専門職員 (70467885)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: 超高感度分離検出 / 非蛍光性化学種 / 紫外レーザー分光 / 光熱変換 / ヘテロダイン検出 / 超高速液体クロマトグラフィー / マイクロチップ / 溶液中化学種

Outline of Final Research Achievements

To enhance versatility of photothermal heterodyne interferometer, a highly sensitive and direct detection method for biologically and environmentally important molecules in liquid solutions with no labeling pretreatment, deep ultraviolet laser at the wavelength of 213 nm is equipped for excitation and the performance of the detection system is evaluated in comparison with a thermal lens detection system. The performance for separation/detection analysis is evaluated in combination with a micro-HPLC and a micro-chip flow device. Non- and less-fluorescent chemicals having no visible light absorption are target species of detection for which fluorometry of the ultrahigh sensitive detection method is quite powerless unless troublesome labelling pretreatment.

Free Research Field

分析化学