2016 Fiscal Year Final Research Report
Molecular mechanism of heme-regulated inhibitor (HRI) under stress conditions
| Project/Area Number |
26340041
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Risk sciences of radiation and chemicals
|
|---|
| Research Institution | Fukushima Medical University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Keywords | ヘム / X線結晶構造解析 |
|---|
| Outline of Final Research Achievements |
Heme-regulated eukaryotic initiation factor 2α (eIF2α) kinase (HRI), functions in response to heme concentration. Under stress conditions, HRI forms stress granules and down-regulates protein synthesis. The molecular mechanism of HRI remained to be established. In the present study, we demonstrate that HRI degradation is inhibited by bortezomib in cultured cells. Using pull-down assay, the interaction between HRI and OGFOD1 are observed. Furthermore, to solve the crystal structure of HRI, insect cell expression systems are constructed.
In addition, we also elucidate the mechanism of ligand recognition of DgcO from Escherichia coli. DgcO, which is also known as YddV or DosC, consists of globin domain, middle domain, and diguanylate cyclase domain. We solved the crystal structure of globin domain of DgcO in the presence of various ligands.
|
| Free Research Field |
生物無機化学
|
