Analysis of trans-plasma membrane molecular machinery between collagen types XV/XVIII and mitochondria

2016 Fiscal Year Final Research Report

• Project/Area Number: 26350892
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied health science
• Research Institution: Okayama University
• Principal Investigator: Momota Ryusuke 岡山大学, 医歯薬学総合研究科, 助教 (80263557)
• Co-Investigator(Renkei-kenkyūsha): OOHASHI Toshitaka 岡山大学, 医歯薬学総合研究科, 教授 (50194262) ; YONEZAWA Tomoko 岡山大学, 医歯薬学総合研究科, 助教 (30304299)
• Research Collaborator: KOMIYAMA Takaaki ; NARASAKI Masahiro
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: XV/XVIII型コラーゲン / ミトコンドリア / 細胞骨格 / 診断法

Outline of Final Research Achievements

To identify the molecules which would compose "the tran-plasma membrane molecular machinery" of collagen types XV/XVIII and mitochondria, we have screened thousands of genes expressed in muscular tissues and identified possible candidate G-protein coupled receptors. In addition, our histological analyses on collagen types XV/XVIII mutant animals revealed abnormal structures of cytoskeletons, which would potentially compromise the mitochondrial and cellular functions. Moreover, we had successfully optimized the immunohistochemical conditions for the anti-collagen XV/XVIII monoclonal antibodies, which enabled us to use variously processed tissue samples to diagnose human diseases caused by defective collagen XV/XVIII.

Free Research Field

解剖学