Mechanism of transplanted adipose tissue stem cells isolated from omentum promoting tissue repair in injured peritoneum

2016 Fiscal Year Final Research Report

• Project/Area Number: 26461260
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Kidney internal medicine
• Research Institution: Juntendo University
• Principal Investigator: HAMADA Chieko 順天堂大学, 医学部, 准教授 (50291662)
• Research Collaborator: Hara Kazuaki
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 腹膜透析 / 組織再生 / 細胞移植 / 細胞形質転換 / 組織線維化 / 抗酸化物質

Outline of Final Research Achievements

Transplanted mesothelial cells into peritoneal cavity aggravated peritoneal injury while progressing peritoneal fibrosis and infiltrating inflammatory cells. However, adipose tissue stem cells (ADSCs) suppressed fibrosis and promote peritoneal repair. Mesothelial cells (MCs) pre-treated with anti-oxidative agent showed resistant to stimulation (peritoneal dialysate) derive epithelial-mesothelial transition (EMT) and production of inflammatory cytokines and growth factors. Furthermore, EMT and production of inflammatory cytokines and growth factors were suppressed in co-culture of ADSCs and MCs under stimulation, and supernatant derived from stimulated ADSCs also inhibited EMT in mesothelial cells against peritoneal dialysate. These results suggested that endocrine effect of ADSCs to mesothelial cells, component cells in peritoneum one of mechanism to promote tissue repair in injured peritoneum.

Free Research Field

腎臓内科学