Modification of radio-sensitivity of cancer by miRNA delivery taking advantage of the exosome.

2016 Fiscal Year Final Research Report

• Project/Area Number: 26461882
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Radiation science
• Research Institution: University of Toyama
• Principal Investigator: Ogawa Ryohei 富山大学, 大学院医学薬学研究部(医学), 准教授 (60334736)
• Co-Investigator(Renkei-kenkyūsha): WATANABE Akihiko 富山大学付属病院, 講師 (20377253) ; MORII Akihiro 黒部市民病院, 泌尿器科, 部長 (20377279)
• Research Collaborator: DOI Nobutaka ニッポンジーン株式会社, 研究員
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: エクソソーム / miRNA / 放射線感受性

Outline of Final Research Achievements

In this research project, we focus on a small vesicle secreted out of a cell called exosome (Ex) as a vehicle to deliver miRNA molecules to a target tissue in the living body. Overexpression of two genes that is involved in biogenesis of Ex resulted in the increase amount of secreted Ex from the cells. In addition, we already obtained two different miRNA like molecules that increase radio-sensitivity of cells when introduced into cells. However, since we have not been able to clone a key gene that is involved in enclosure of RNA molecules into Ex, we could not examine the function of the gene.

Free Research Field

放射線生物学