2016 Fiscal Year Final Research Report
Translational research of the regenerative treatment of using human hepatic stem cells which were frozen by super-freezing program
| Project/Area Number |
26461921
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 肝再生 / 幹細胞 / 細胞バンク / 超過冷却 |
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| Outline of Final Research Achievements |
We aimed at establishing the cell harvest method for human hepatocyte with safety and technical issues. We reported an effective method for donor cell harvests with video presentation. We also elucidate clinical values of lactate levels during the cell harvesting. We developed a standard for evaluating postoperative complications. We investigate a cell harvest method regarding anatomical location of the donor cells. BCAA plays an important role to maintain nutritional balance. We also aimed at establishing a banking hepatic stem cells and investigate cell preparation and cell nature. In the research period, we success to separate and culture hepatic stem cells. We tried to protect the method by a patent. Finally, we found an antigen to identify hepatic stem cells and select specific cells for regenerative therapy.
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| Free Research Field |
消化器外科
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