2016 Fiscal Year Final Research Report
Investigation on development of therapeutic method for infectious diseases based on microbiome analysis
| Project/Area Number |
26462504
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Aichi Medical University |
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Principal Investigator |
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| Research Collaborator |
MATSUMOTO Asami
IZUMI Koji
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 網羅的細菌叢解析 / 16Sメタゲノム解析法 / 次世代シークエンサー / 腟内細菌叢 / 細菌性腟症 / 早産 / Lactobacillus |
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| Outline of Final Research Achievements |
The genus Lactobacillus species prevent invasion and proliferation of pathogenic bacteria in the vaginal flora, while its composition changes with health condition, sexual intercourse and pregnancy. We collected vaginal secretions of non-pregnant women, pregnant women and commercial sex worker (CSW). Vaginal microbiota was analyzed by Terminal Restriction Fragment Length Polymorphism (T-RFLP) method and 16S metagenomic analysis method, and based on the results of vaginal microbiota composition ratios, cluster analysis and diversity analysis was carried out. In addition, vaginal secretions were cultured and the isolation rate of Lactobacillus species was calculated. It has become clear that controlling female vaginal flora by using probiotics and so on for prevention of female genital infections and premature birth could be a new treatment method for infectious diseases.
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| Free Research Field |
感染症学、化学療法学、感染制御学、臨床微生物学、産婦人科感染症学、周産期医学、性感染症学
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