Multi-faceted analysis of cause investigation on refractory periapical periodontitis

2016 Fiscal Year Final Research Report

• Project/Area Number: 26462880
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Conservative dentistry
• Research Institution: Osaka University
• Principal Investigator: NAGAYAMA TOMOTAKA 大阪大学, 歯学研究科, 招聘教員 (60456944)
• Co-Investigator(Renkei-kenkyūsha): ASAHI YOKO 大阪大学, 大学院歯学研究科, 助教 (50456943)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 歯内療法学 / 細菌 / バイオフィルム / 難治性根尖性歯周炎 / 16s rRNA遺伝子解析法 / CBCT / 咬合性外傷

Outline of Final Research Achievements

To clarify the cause of refractory periapical periodontitis, bacterial biofilm on root apex obtained by apicoectomy or tooth extraction was investigated by PCR-based 16s rRNA gene assay. In addition, we performed morphologically examination by scanning electron microscope and clinical examination in oreder to analyze pathology from both the basic and clinical aspects. As a result, we demonstrated the various patterns of pathology on refractory periapical periodontitis conbined with multiple factors such as the 3D shape of apical lesion, anatomical form of root apex and occlusal trauma in addition to extraradicular biofilm.

Free Research Field

歯科保存学