Generation of mosaic model of epidermolysis bullosa by inactivation of X chromosome.

2014 Fiscal Year Final Research Report

• Project/Area Number: 26670516
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Dermatology
• Research Institution: Hokkaido University
• Principal Investigator: NISHIE Wataru 北海道大学, 大学病院, 講師 (20443955)
• Co-Investigator(Kenkyū-buntansha): NATSUGA Ken 北海道大学, 大学病院, 助教 (70645457)
• Project Period (FY): 2014-04-01 – 2015-03-31
• Keywords: 皮膚病態学

Outline of Final Research Achievements

The popups of this study is to generate a mosaic model of epidermolysis bullosa by inactivation of X chromosome. We have generated transgenic (Tg) mice with tetracycline response plasmid expressing human type XVII collagen (COL17) cDNA, and then crossed with the Tg mice expressing reverse tetracycline transactivator driven under keratin 14 promoter on X-chromosome. When primary keratinocytes from the double Tg mice were treated with doxycycline in culture, increased human COL17 gene expression was observed; however, COL17 protein expression was not induced. These results indicate that it may be challenging for this method to obtain mice with high inducible level of COL17, which is needed to be improved.

Free Research Field

皮膚科学