Synthetic study of cysteine rich proteins

2015 Fiscal Year Final Research Report

• Project/Area Number: 26810088
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Bio-related chemistry
• Research Institution: Nagoya University
• Principal Investigator: Oishi Shunsuke 名古屋大学, トランスフォーマティブ生命分子研究所, 助教 (80707795)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 有機合成化学 / タンパク質の化学合成 / ケミカルバイオロジー / 植物化学 / 環状ペプチド / 生理活性中分子

Outline of Final Research Achievements

We synthesized LURE pollen tube attracting proteins, which plays an important role in plant reproduction. The synthesis was accomplished by KAHA peptide ligation reaction of two peptide segments obtained by Fmoc solid phase peptide synthesis. We found that the ligation reaction proceed efficiently by addition of reducing agent (TCEP).
We also synthesized cyclic peptides having partial sequence of LURE proteins and the cyclic peptides exhibited the same biological activity as the parent LURE proteins.

Free Research Field

有機合成化学、ケミカルバイオロジー、タンパク質化学