Elucidation of pectin biosynthesis mechanism involving novel Golgi-localized polygalacturonase

2016 Fiscal Year Final Research Report

• Project/Area Number: 26840034
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Functional biochemistry
• Research Institution: Osaka University
• Principal Investigator: OHASHI TAKAO 大阪大学, 生物工学国際交流センター, 助教 (10597876)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: ペクチン / 植物細胞壁 / ポリガラクツロナーゼ / 分裂酵母 / シロイヌナズナ

Outline of Final Research Achievements

Polygalacturonase is pectin-hydrolyzing enzyme. It was thought that the polygalacturonase was secreted and localized at cell wall, and degrade the assembled pectic polysaccharides. Especially the cell wall-type polygalacturonases involved in the fruit maturation, leave and fruit abscissions and pollen maturation were well studied. In this study, I found the novel Golgi-localized polygalacturonase and enzymatically characterized. In addition, I attempted to elucidate the physiological functions of the Golgi-localized polygalacturonase using Arabidopsis T-DNA mutants.

Free Research Field

糖鎖生物学