2015 Fiscal Year Final Research Report
New sarcoma therapy targeted to acidic microenvironment created by Vacuolar H+-ATPase (V-ATPase)
| Project/Area Number |
26861196
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Orthopaedic surgery
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| Research Institution | The University of Tokushima |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | 酸性環境 / 肉腫 / V型ATPase |
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| Outline of Final Research Achievements |
To examine whether acidic microenvironment promote sarcoma activity, SW872 liposarcoma cells were cultured in pH7.4 or pH 6.4 medium. SW872 cells treated with acidic medium (pH 6.4) showed increased invasion and proliferation activity.
Next, We investigated expression of the vacuolar-type H-ATPase (V-ATPase) which has been shown to cause extracellular acidification by pumping protons. The isoform of a3, E2, F, G2, H were increased in liposarcoma (malignant tumor) compared by lipoma (benign tumor).
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| Free Research Field |
骨・軟部腫瘍
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