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An Experimental Study of Photodynamic Therapy Using Doxycycline Derivertives on Rabbit Atherosclerosis Model

Research Project

Project/Area Number 03557059
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Cerebral neurosurgery
Research InstitutionTOHOKU UNIVERSITY

Principal Investigator

YOSHIMOTO Takashi  Tohoku Univ., Medicine, Professor and Chairman, 医学部, 教授 (50091765)

Co-Investigator(Kenkyū-buntansha) UENOHARA Hiroshi  Sendai Ntl.Hospital, Docter, 医師
KAYAMA Takamasa  Tohoku Univ., Medicine, Instructer, 医学部, 講師 (50142972)
SATOH Syunichi  Tohoku Univ., Inst.for advanced material processing, Instructer, 素材工学研究所, 講師 (30162431)
TAGUCHI Yoshio  Tohoku Univ., Foreign Student Center, Professor, 留学生センター, 教授 (70004885)
溝井 和夫  東北大学, 医学部, 助教授 (70157519)
稲場 文男  東北大学, 電気通信研究所, 教授 (90006213)
Project Period (FY) 1991 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥17,700,000 (Direct Cost: ¥17,700,000)
Fiscal Year 1993: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1992: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1991: ¥16,300,000 (Direct Cost: ¥16,300,000)
KeywordsPhotodynamic therapy(PDT) / Atherosclerosis / Obstructive cerebrovascular diseases / Tetracycline derivaratives / Laser angioplsty / Intravascular surgery / 蛍光診断 / レ-ザ-血管形成術
Research Abstract

Using doxycycline(Dc : tetracycline derivertive) as a photosensitizer, the authers experimentally evaluated the possibility of photodynamic therapy(PDT) for atheromatous plaques on rabbit's models.
In order to define the acceptable laser power for PDT, we examined previously the acute effects of 355nm laser for resected plaques that induced in rabbit's abdominal aorta. Treated with or without Dc, the laser was radiated in the power of 0.5, 1, 1.5, 2, 3 or 6W/cm^2, and the effect was evaluated as vapolizetion time.
Based on this result, we decided to try the PDT to the rabbit's models with 0.3/cm^2 of 355nm laser source. The PDT group that was 12 hours after the infusion of 60mg/kg Dc and control group without Dc underwent surgical exposure of their abdominal aortas in which plaques were induced, then the 355nm laser was radiated for 3 minutes directly to the most severe one. They were killed at 1 day, 1 week and 2 months after the treatment, and their pathological changes were examined microscopically.
In result, the effects of PDT were presented as some necrosis of the plaques at 1 week after the treatment, and reduction or disappearance of them at 2 months after. Compared to the control group, this process were proper to the PDT group in our experience, and we thought that Dc was one of the photosensitizers which were worth to investigate for clinical use.

Report

(4 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • 1991 Annual Research Report
  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] 藤森清: "動脈硬化病変に対する光力学的療法の基礎研究:Tetracycline誘導体" 第11回日本レーザー医学会大会論文集. 321-324 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 上之原広司: "動脈硬化病変に対する光力学的療法の基礎研究" 第6回日本脳神経血管内手術研究会講演集. 63-67 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 藤森清: "動脈硬化病変の血管内蛍光診断に関する基礎的研究" 第12回日本レーザー医学会大会論文集. 231-234 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 藤森清: "動脈硬化病変に対する光力学的治療の長期効果の検討" 第13回日本レーザー医学会大会論文集. 479-482 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 上之原広司: "血管内超音波診断" 循環科学. 13. 1110-1114 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 吉田昌弘: "閉塞性脳血管障害に対する光力学的療法:光増感剤としてのテトラサイクリン系抗性物質の有用性" 第14回日本レーザー医学会大会論文集. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] K.Fujimori: "An Experimental Study of Photodynamic Therapy(PDT) of Atherosclerosis ; With Tetracycline Derivatives." Proc.of the 11th Annual Meeting of the Japan Society for Laser Medicine.321-324 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] H.Uenohara: "An Experimental Study of Photodynamic Therapy(PDT) of Atherosclerosis." Proc.of the 5th Workshop on Japanese Intravascular Neurosurgery.63-67 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] K.Fujimori: "The pathological correlation of experimental atheromatous plaques with their fluorescent spectra after doxycycline application." Proc.of the 12th Annual Meeting of the Japan Society for Laser Medicine.231-234 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] K.Fujimori: "Photodynamic therapy using doxycycline and its long-term effect on rabbit model." Proc.of the 13th Annual Meeting of the Japan Society for Laser Medicine.479-482 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] H.Uenohara: "Intravascular ultrasound image." Junkan Kagaku. 13. 1110-1114 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 藤森清: "動脈硬化病変に対する光力学的療法の基礎研究;Tetracycline誘導体" 第11回日本レーザー医学会大会論文集. 321-324 (1990)

    • Related Report
      1993 Annual Research Report
  • [Publications] 藤森清: "動脈硬化病変の血管内蛍光診断に関する基礎的研究" 第12回日本レーザー医学会大会論文集. 231-234 (1991)

    • Related Report
      1993 Annual Research Report
  • [Publications] 藤森清: "動脈硬化病変に対する光力学的治療の長期効果の検討" 第13回日本レーザー医学会大会論文集. 479-482 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] 上之原広司: "動脈硬化病変に対する光力学的療法の基礎的研究" 第6回日本脳神経血管内手術研究会講演集. 63-67 (1990)

    • Related Report
      1993 Annual Research Report
  • [Publications] 上之原広司: "血管内超音波診断" 循環科学. 13. 1110-1114 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 吉田昌弘: "閉塞性脳血管障害に対する光力学的療法-光増感物質としてのテトラサイクリン系抗生物質の有用性" 第14回日本レーザー医学会大会論文集. (in press).

    • Related Report
      1993 Annual Research Report
  • [Publications] 藤森 清: "動脈硬化病変に対する光力学的療法の基礎研究;Tetracyctinc誘導体" 第11回日本レーザー医学会大会論文集. 321-324 (1990)

    • Related Report
      1992 Annual Research Report
  • [Publications] 藤森 清: "動脈硬化病変の血管内蛍光診断に関する基礎的研究" 第12回日本レーザー医学会大会論文集. 231-234 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] 藤森 清: "動脈硬化病変に対する光力学的治療の長期効果の検検" 第13回日本レーザー医学会大会論文集.

    • Related Report
      1992 Annual Research Report
  • [Publications] 藤森 清: "経皮的血管形成術における血管内エコー診断システムの使用経験" 第7回日本脳神経血管内手術研究会講演集. 227-232 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 上之原 広司: "動脈硬化病変に対する光力学的療法の基礎的研究" 第6回日本脳神経血管内手術研究会講演集. 63-67 (1990)

    • Related Report
      1992 Annual Research Report
  • [Publications] 上之原 広司: "動脈硬化巣に対する光力学的治療の長期効果,実験的検討" 第8回日本脳神経血管内手術研究会講演集.

    • Related Report
      1992 Annual Research Report
  • [Publications] 吉本 高志: "閉塞性疾患の外科治療II治療の実際" 第23回日本医学会総会会誌. 1. 368- (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 藤森 清: "動脈硬化病変の血管内蛍光診断に関する基礎的研究" 第12回日本レ-ザ-医学会大会論文集. (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 藤森 清: "動脈硬化病変に対する光力学的療法の基礎研究;Tetracycliue誘導体" 第11回日本レ-ザ-医学会大会論文集. 321-324 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] 上之原 広司: "動脈硬化病変に対する光力学的療法の基礎研究" 第6回日本脳神経血管内手術研究会講演集.

    • Related Report
      1991 Annual Research Report
  • [Publications] 吉本 高志: "閉塞性脳血管障害の治療;脳梗塞急性期の外科治療-1)" 高倉公朋監修(株)現代医療社発行, 228 (1989)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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