| Project/Area Number |
08672367
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
矯正・小児・社会系歯学
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
MORISHITA Masayuki Hiroshima University School of Dentistry, Assistant Professor, 歯学部, 講師 (90166405)
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| Co-Investigator(Kenkyū-buntansha) |
SHIMAZU Atsushi Hiroshima University School of Dentistry, Assistant, 歯学部, 助手 (10274094)
IWAMOTO Yoshifumi Hiroshima University School of Dentistry, Professor, 歯学部, 教授 (90034165)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1997: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1996: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | sex hormones / 17beta-estradiol / periodontal ligament cells / periodontal disease / エストロゲン / 歯根膜線維芽細胞 / エストロゲンレセプター |
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| Research Abstract |
A primary objective in the treatment of periodontal disease is the regeneration of the mineralize and soft connective tissue. PDL cells produce mineralized nodules in vitro which is one of the important functions of PDL cells for regenerative therapy. The purpose of this study was to investigate the effects of estradiol on mineralized nodule formation by human PDL cells. PDL cells were ob-tained from healthy donors and maintained in DMEM with 10% fetal bovine serum. Serum-free medium was used when the effects of estradiol were tested. ALP activity in the supernatant of cells disrupted by sonication was analyzed spectrophotometrically. The formation of mineralized nodules was assessed by staining the PDL cells with alizarine red and counting the number of nodules. at Estradiol 20 ng/ml significantly enhanced the ALP activity and mineralized nodule formation compared to the control. These results suggested that estrogen status may modify the regenerative activity of periodontal tissue.
Periodontal ligament (PDL) cells are known to have osteoblast-like features and are capable of differentiating into osteogenic cells. As human osteoblasts express estrogen receptor mRNA, it is possible that PDL cells also express these receptors. However, conflicting findings have been reported regarding the presence of oestrogen receptor mRNA in PDL cells. To determine whethe PDL cells express oestrogen receptor mRNA, reverse transcriptase-polyrmerase chain reaction (RT-PCR) was performed using two different primers. Periodontal ligament (PDL) cells were obtained from a healthy periodontal ligament of premolar extracted for orthodontic reasons. The humar breast adenocarcinoma cell line MCF7 was used as a positive control. Expression of oestrogen receptor mRNA in PDL cells was detected using one of the primers with less intensity compared to MCF7. Southern hybridization confirmed these results. Our findings suggest that PDL cells express oestrogen receptor mRNA at low levels.
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