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APPLICATION OF BGL-2 ANTISENSE OLIGO-DNA FOR GENE THERAPY TO UROLOGICAL CANCER.

Research Project

Project/Area Number 10470333
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Urology
Research InstitutionNIIGATA UNIVERSITY

Principal Investigator

TOMITA Yoshihiko  Faculty of Medicine, NIIGATA UNIVERSITY associate, 医学部, 助教授 (90237123)

Co-Investigator(Kenkyū-buntansha) TANIKAWA Toshiki  University Hospital, NIIGATA UNIVERSITY Lecturer, 医学部・附属病院, 講師 (70236686)
斉藤 俊弘  新潟大学, 医学部附属病院, 助手 (40251813)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥10,700,000 (Direct Cost: ¥10,700,000)
Fiscal Year 1999: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1998: ¥8,600,000 (Direct Cost: ¥8,600,000)
Keywordsrenal cell cancer / bcl-2 / antisense oligonucleotide / gene therapy / アンチセンスオリゴDN4 / アポトーシス
Research Abstract

We have previously shown that Bcl-2 expression was negative prognostic factor in transitional cell cancer (TCC), and that TCC cell lines expressing high levels of Bcl-2 are resistant to Adriamycin triggered apoptosis. Here we examined antisense oligonucleotide - mediated downregulation of Bcl-2 expression and its effect on sensitivity to ADM treatment in T24 cells. Treatment of T24 cells with 20 μM of bcl-2 antisense PODN reduced the Bcl-2 protein level. Combined administration with Adriamycin resulted in synergistic cytotoxicity, accompanied with a 2.4-fold increase in DEVD-specific caspase activity. The finding provides evidence that Bcl-2 expression may be critical for maintaining the drug resistance of TCC. bcl-2 antisense PODN might be useful means for. overcoming drug resistance in highly malignant TCC,

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] 冨田 善彦: "アポトーシスの機構と尿路性器癌"泌尿器外科. 11. 1209-1214 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 冨田 善彦: "泌尿器科領域におけるアポトーシス研究の意義"泌尿器外科. 12. 365-367 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 冨田 善彦: "腫瘍免疫とアポトーシス"腎と透析. 45. 625-631 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kawasaki, T.: "Infrequent alteration of p53 pathway inmetasiatic renal cell carcinoma"Oncology Reports. 6. 329-333 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Bilim, V.: "Absence of frame shift mutations in the baxgene in RCC and TCC"Anticancer Research. 18. 1655-1660 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Bilim, V.: "Variable phenotype in benign and malignant lesions of urothelium"Cancer Letter. 128. 87-92 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Bilim, V.: "Caspase involved synergistic cytotoxicity of bcl-2 antisense ODN and ADR"Cancer Letter. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tomita, Y.: "Urological cancer and apoptotic machinery."Hinyokigeka. 11. 1209-1214 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tomita, Y.: "Significance of apoptosis research in Urological field."Hinyokigeka. 12. 365-367 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tomita, Y.: "Tumor Immunology and apoptosis."Jin to Toseki. 45. 625-631 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kawasaki, T., V. Bilim, K. Takahashi, Y. Tomita.: "Infrequent alteration of p53 pathway in metastatic renal cell carcinoma."Oncology Reports. 329-333 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Bilim, V., T. Kawasaki, K. Takahashi Y. Tomita,.: "Absence of frame shift mutations in the bax gene in renal cell cancer (RCC) and transitional cell cancer (TCC)"Anti cancer Res.. 18. 1655-1660 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Bilim, V., Y. Tomita, T. Kawasaki, M. Takeda, K. Takahashi.: "Variable phenotype in benign and malignant lesions of urothelium."Cancer Lett.. 128. 87-92 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Bilim, V., T. Kawasaki, K. Takahashi Y. Tomita,.: "Caspase involved synergistic cytotoxicity of bcl-2 antisense ODN and ADR"Cancer letter. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 冨田 善彦: "アポトーシスの機構と尿路性器癌"泌尿器外科. 11. 1209-1214 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] 冨田 善彦: "泌尿器科領域におけるアポトーシス研究の意義"泌尿器外科. 12. 365-367 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 冨田 善彦: "腫瘍免疫とアポトーシス"腎と透析. 45. 625-631 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kawasaki, T.: "Infrequent alteration of p53 pathway in metastatic renal cell carcinoma"Oncology Reports. 6. 329-333 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Bilim, V.: "Absence of frame shift mutations in the bax gene in RCC and TCC"Anticancer Research. 18. 1655-1660 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Bilim, V.: "Variable phenotype in benign and malignant lesions of cuothelium"Cancer Letter. 128. 87-92 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Bilim, V.: "Caspase involved synergistic cytotoxicity of bcl-2 antisese ODN and ADR"Cancer Letter. (印刷中).

    • Related Report
      1999 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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