| Project/Area Number |
11672309
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Tokai University |
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Principal Investigator |
ANDO Yasuhiko Tokai University, School of Medicine, Professor, 医学部, 教授 (50051470)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2000: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1999: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | Flow Cytometric / Sleep apnea syndrome (SAS) / Activated platelets / CD62P / PAC-1 / 睡眠時無呼吸症候群患者 |
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| Research Abstract |
Sleep apnea syndrome (SAS) is associated with increased risk of thrombotic disorders such as cerebral thrombosis and myocardial infarction. In this study we sought to elucidate the role of platelet activation in the evolution of thrombotic complications in SAS.Sixty two patients with SAS and nineteen normal volunteers were enrolled in this study. Platelet activation was assessed by three color flow cytometry using activation-dependent monoclonal antibodies (MoAb) in citrated whole blood. One fluorescent reagent, PerCP-MoAb-CD61 was used to identify all the platelets while two other fluorescent reagents, FITC-PAC 1 and PE-MoAb-CD62P were used to detect activated platelets.
The percentage of platelets positive for activation dependent MoAb was significantly higher in patients with SAS (PAC 1 63.5±24.8%, CD 6 2P 4.8±5.3%, mean±SD), as compared with healthy control subjects (PAC 1 18.4±9.4%, CD 6 2P 0.8±0.5%, p<0.0001). In conclusion, both activation dependent markers are increasingly expressed on the platelet surface in SAS patients. It can be suspected platelet activation might play a crucial role in the pathogenesis of thrombotic complications in SAS.
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