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Development of new strategy in surgical oncology introduced with regulation of micometastasis

Research Project

Project/Area Number 12671156
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General surgery
Research InstitutionOsaka University

Principal Investigator

FUJIWARA Yoshiyuki  Osaka University, Graduate School of Medicine, Assistant Professor, 医学系研究科, 助手 (40314330)

Co-Investigator(Kenkyū-buntansha) YONO Masahiko  Osaka University, Graduate School of Medicine, Associate Professor, 医学系研究科, 助教授 (70273646)
NAKAMORI Shoji  Osaka University, Graduate School of Medicine, Lecturer, 医学系研究科, 講師 (70294080)
MONDEN Morito  Osaka University, Graduate School of Medicine, Professor, 医学系研究科, 教授 (00127309)
塩崎 均  大阪大学, 医学系研究科, 助教授 (70144475)
井上 雅智  大阪大学, 医学系研究科, 講師 (80232560)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2001: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2000: ¥2,000,000 (Direct Cost: ¥2,000,000)
Keywordsgenetic diagnosis / micrometastasis / RT-PCR / gastrointestinal cancer / breast cancer / lung cancer / intra - operative diagnosis / 食道癌 / 胃癌 / リンパ節転移 / 腹腔洗浄液
Research Abstract

In this study, we have developed the genetic diagnosis with multiple-marker RT-PCR assay in order to detect micrometastases in solid tumors such as gastric, esophageal, colorectal, lung, pancreas, liver, bile duct and breast carcinomas.
With the use of surgical specimens such as lymph node and blood, we demonstrated the usefulness and significance of this assay in these types of cancer. Furthermore, we have developed the quality of control in this genetic detection, including the sampling procedure without cross-contamination for introducing genetic diagnosis into the clinical use. We also developed intra-operative genetic diagnosis of lymph nodes for the selection of cervical lymphadenectomy in esophageal cancer surgery.
We applied this rapid genetic diagnosis for 72 patients performed with esophageal cancer surgery by now and showed the usefulness and significance of this genetic diagnosis as the operative indication of cervical lymphadenectomy.
We will improve the genetic system (more rapid and more convenient way) to apply it for many kinds of cancer surgery in the future. Furthermore, precise molecular staging will detect the high-risk group for cancer recurrence, which will be subjected to the additional therapy.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] Miyamoto A., Fujiwara Y. et al.: "Development of a multiple-marker RT-PCR assay for detection of micrometastases of hepatocellular carcinoma"Dig Dis Sci. 45. 1376-1382 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Miyake Y., Fujiwara Y., et al.: "Quantification of micrometastases in lymph nodes of colorectal cancer using real-time fluorescence polymerase chain reaction"Int J Oncol. 16. 289-293 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Fujiwara Y. et al.: "Prevention of cross-contamination during sampling procedure in molecular detection for cancer micrometastasis"Cancer Letters. 153. 109-111 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Miyake Y., Fujiwara Y. et al.: "Extensive micrometastases to lymph nodes as a marker for rapid recurrence of colorectal cancer : A study of lymphatic mapping1"Clin Can Res. 7. 1350-1357 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Iwao K., Fujiwara Y. et al.: "Isolation of a novel human lung-specific gene, lunx, a potential molecular marker for detection of micrometastasis in non-small-cell lung cancer"Int J Cancer. 91. 433-437 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Okada Y., Fujiwara Y. et al.: "Genetic detection of lymph node micrometastases in patients with gastric carcinoma by multiple-marker reverse transcriptase-polymerase chain reaction assay"Cancer. 92. 2056-2064 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Miyamoto A., Fujiwara Y. et al.: "Development of a multiple-marker RT-PCR assay for detection of micrometastases of hepatocellular carcinoma"Dig Dis Sci. 45. 1376-1382 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Miyake Y., Fujiwara Y. et al.: "Quantification of micrometastases in lymph nodes of colorectal cancer using real-time fluorescence polymerase chain reaction"Int J Oncol. 16. 289-293 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Fujiwara Y. et al.: "Prevention of cross-contamination during sampling procedure in molecular detection lor cancer micrometastasis"Cancer Letters. 153. 109-111 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Miyake K, FujiwaraY. et al.: "Extensive micrometastases to lymph nodes as a marker for rapid recurrence of colorectal cancer : A study of lymphatic mapping1"Clin Can Res. 7. 1350-1357 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Iwao K., Fujiwara Y. et al.: "Isolation of a novel human lung-specific gene, lunx, a potential molecular marker for detection of micrometastasis in non-small-cell lung cancer"Int J Cancer. 91. 433-437 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Okada Y., Fujiwara Y. et al.: "Genetic detection of lymph node micrometastases in patients with gastric carcinoma by multiple -marker reverse transcriptase-polymerase chain reaction assay"Cancer. 92. 2056-2064 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Miyamoto A., Fujiwara Y.et al.: "Clinical application of quantitative analysis for detection of hematogenous spreadof hepatocellular carcinoma by real-time PCR"Int J Oncol. 18. 527-532 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Takemoto H., Fujiwara Y.et al.: "Localization of iqgapl is inversely correlated with intercellular adhesion mediated by E-Cadherin in gastric cancers"Int J Cancer. 91. 783-788 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Iwao K., Fujiwara Y.et al.: "Isolation of a novel human lung-specific gene, lunx, a potential molecular marker for detection of micrometastasis in non-small-cell lung cancer"Int J Cancer. 91. 433-437 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Miyake Y., Fujiwara Y.et al.: "Extensive micrometastases to lymph nodes as a marker for rapid recurrence of colorectal cancer : A study of lymphatic mappingl"Clin Can Res. 7. 1350-1357 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ooka M., Fujiwara Y.et al.: "Bone marrow micrometastases detected by RT-PCR for mammaglobin can be an alternative prognostic factor for breast cancer"Breast Cancer Res and Treat. 67. 169-175 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kaneko K., Fujiwara Y.et al.: "Detection of peritoneal micrometastases of gastric carcinoma with green fluorescent protein and carcinoembrvonic antigen promoter"Cancer Res. 61. 5570-5574 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Taback B., Fujiwara Y.et al.: "Prognostic significance of circulating microsatellite markers in the plasma of melanoma patients"Cancer Res. 61. 5723-5726 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Okada Y., Fujiwara Y.et al.: "Genetic detection of lymph node micrometastases in patients with gastric carcinoma by multiple-marker reverse transcriptase-polymerase chain reaction assay"Cancer. 92. 2056-2064 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Iwao K.,Watanabe T.,Fujiwara Y., et al.: "Isolation of a novel human lung-specific gene, LUNX, a potential molecular marker for detection of micrometastasis in non-small cell lung cancer."Int.J.Cancer. 91. 433-437 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Miyamoto A.,Fujiwara Y., et al.: "Development of a multiple marker RT-PCR assay for detection of micrometastases of hepatocellular carcinoma."Dig.Dis.Sci.. 45. 1376-1382 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Okami J.,Dohno K.,Fujiwara Y., et al.: "Genetic Detection for Micrometastasis in Lymph Node of Biliary Tract Carcinoma."Clin.Cancer Res.. 6. 2326-2332 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Bilichik A.,Miyashiro M.,Fujiwara Y., et al.: "Molecular detection of metastatic pancreatic carcinoma cells using a multimarker reverse transcriptase-polymerase chain reaction assay."Cancer. 88. 1037-1044 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Fujiwara Y.,Ooka M., et al.: "Prevention of cross-contamination during sampling procedure in molecular detection for cancer micrometastasis."Cancer Letters. 29;153(1-2). 109-111 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Miyake Y,Fujiwara Y, et al.: "Quantification of micrometastases in lymph nodes of colorectal cancer using real-time fluorescence polymerase chain reaction."Int J Oncol. 16(2). 289-293 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Aihara T.,Fujiwara Y., et al.: "Mammaglobin B gene as a novel marker for lymph node micrometastasis in patients with abdominal cancers."Cancer Letters. 150. 79-84

    • Related Report
      2000 Annual Research Report
  • [Publications] Fujiwara Y.,Sugita Y., et al.: "Assessment of Stanniocalcin-1 mRNA as a molecular marker for micrometastases of various human cancers."Int.J.Oncol.. 16. 799-804

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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