Molecular and pathological analysis of CVD-interstitial pneumonia by using lung tissue-infiltrating T lymphocytes
| Project/Area Number |
14570561
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Dokkyo University School of Medicine |
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Principal Investigator |
TAKEDA Akira Dokkyo University School of Medicine, Dept. of Medicine, Assistant professor, 医学部, 講師 (90155002)
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| Co-Investigator(Kenkyū-buntansha) |
福島 康次 獨協医科大学, 医学部, 講師 (00254996)
沼尾 利郎 獨協医科大学, 医学部, 講師 (60172748)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2003: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2002: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | Interstitial pneumonia / Collagen diseases / T cells |
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| Research Abstract |
Interstitial pneumonia (IP) associated with polymyositis/dermatomyositis (PM/DM) has been perceived as an life-threatening complication of the connective tissue disease.
Since the pathogenesis of IP remains unclear, the treatment has been conventional and often inadequate.
However, the precise characterization of the lymphocytes infiltrating the lung tissue may provide clue to understanding the immune-mediated mechanisms for IP.
We examined the phenotypic profiles of lung tissue-infiltrating lymphocytes as well as the expression of cytokines in the affected lung tissue.
TCR-repertoire study with RT-PCR was also employed using lung derived RNA and the results suggested oligoclonal expansion of particular T cell populations.
Immunohistochemical analysis of VATS biopsies from eight patients with PM/DM demonstrated that the lymphocytes in the lesions are predominantly T cells a most of which are CD4 positive, and the study also showed strong expression of IFNγ in the tissue with lesser levels of 1L4 and 1L5.
These data suggest that the pivotal role of T cells which may contribute to the development of IP associated with PM/DM through the proinflammatory cytokine-mediated mechanism.
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Report
(3 results)
Research Products
(7 results)
