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New methods of lung cancer screening using biomarker analysis in blood and sputum

Research Project

Project/Area Number 15591494
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Thoracic surgery
Research InstitutionTokyo Medical University

Principal Investigator

IKEDA Norihiko  Tokyo Medical University, Medicine, Assistant Professor, 医学部, 講師 (70246205)

Co-Investigator(Kenkyū-buntansha) HIRANO Takashi  Tokyo Medical University, Medicine, Assistant Professor, 医学部, 講師 (30238381)
HONDA Hidetoshi  Tokyo Medical University, Medicine, Stuff, 医学部, 助手 (70246297)
KATO Harubumi  Tokyo Medical University, Medicine, Professor, 医学部, 教授 (20074768)
本多 英俊  東京医科大学, 医学部, 助手 (60349503)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2004: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2003: ¥1,100,000 (Direct Cost: ¥1,100,000)
Keywordslung cancer / biomarker / p16 / methylation / cytometry / early cancer / screening / P16 / 癌遺伝子 / 癌抑制遺伝子 / 肺癌検診
Research Abstract

Our biomarker research was classified into two projects as described below.
(1)Detection of aberrant p16 promoter methylation of tumor suppressor genes in serum DNA from non-small cell lung cancer patients using real-time PCR.
(Materials and Methods) Serum samples were obtained from 95 patients with non-small cell lung cancer and 32 normal controls and evaluated the quantity of p16 promoter methylation using real-time PCR.
(Results) Aberrant p16 promoter methylation of the tumor suppressor gene was detected in 25 of 95 NSCLC patients (mean:18.613±5.090), but only 2 of 32 normal control (mean: 1.258±0.871). There was a statistical difference between non small cell lung cancer patients and normal control (t-test: p<0.003). Sensitivity was 26.3%, specificity, 93.8%, accuracy, 43.3%, and positive predictive value 92.6%, respectively.
(Conclusion) Detection of aberrant p16 promoter hypermethylation in serum using real time PCR appears useful for lung cancer diagnosis and early detection.
(2)Cytometry analysis of normal cells for the evaluation of lung cancer risk
(Material and Methods) Sputum samples were collected from 52 lung cancer cases and 54 controls. Also buccal smears were collected from 69 lung cancer cases and 45 controls. These samples were analyzed by high resolution cytometry and 100 nuclear features were evaluated in each cell.
(Results) Statistical analysis revealed normal cells from cancer group were well distinguished from normal cells from control group by the combination of 10 features. Based on this result, test set program to evaluate the lung cancer risk was created.
Our two projects demonstrated the feasibility of biomarker research using blood, sputum and buccal cells.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (7 results)

All 2004 2003

All Journal Article (7 results)

  • [Journal Article] A clinicopathologicalstudy of resected adenocarcinoma 2 cm or less in diameter2004

    • Author(s)
      池田徳彦
    • Journal Title

      Annals of Thoracic Surgery 78

      Pages: 1011-1016

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A clinicopathologicalstudy of resected adenocarcinoma 2 cm or less in diameter2004

    • Author(s)
      Ikeda N, Maeda J, Yashima K, Tsuboi M, Kato H, Akada S, Okada S
    • Journal Title

      Annals of Thoracic Surgery 78

      Pages: 1011-1016

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A clinicopathological study of resected adenocarcinoma 2 cm or less in diameter2004

    • Author(s)
      池田徳彦
    • Journal Title

      Annals of Thoracic Surgery 78

      Pages: 1011-1016

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Usefulness of TA02 (napsin A) to distinguish lung adenocarcinoma from metastatic lung adenocarcinoma.2003

    • Author(s)
      平野 隆
    • Journal Title

      Lung Cancer 41・2

      Pages: 155-162

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 中心型早期は胃がんの診断と治療2003

    • Author(s)
      池田徳彦
    • Journal Title

      病理と臨床 21・5

      Pages: 481-486

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Usefulness of TA02 (napsin A) to distinguish primary lung adenocarcinoma from metastatic lung adenocarcinoma.2003

    • Author(s)
      Hirano T, Gong Y, Yoshida K, Kato Y, Yashima K, Maeda M, Nakagawa A, Fujioka K, Ohira T, Ikeda N, Ebihara Y, Auer G, Kato H.
    • Journal Title

      Lung Cancer 41(2)

      Pages: 155-162

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Diagnosis and Treatment of central type early stage lung cancer (in Japanese)2003

    • Author(s)
      Ikeda N, Kato Y, Yashima K, Kato H
    • Journal Title

      Patholgy and Clinic 21(5)

      Pages: 481-486

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary

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Published: 2003-04-01   Modified: 2016-04-21  

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