| Project/Area Number |
15591534
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Kumamoto University |
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Principal Investigator |
MORIOKA Motohiro Kumamoto University, Neurosurgery, Instructor, 医学部附属病院, 講師 (20295140)
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| Co-Investigator(Kenkyū-buntansha) |
HAMADA Jun-ichiro Kumamoto University, Neurosurgery, Instructor, 医学部附属病院, 講師 (40253752)
GOTO Satoshi Kumamoto University, Neurosurgery, Assistant Professor, 大学院・医学薬学研究部, 助教授 (50240916)
KAI Yutaka Kumamoto University, Neurosurgery, Assistant, 大学院・医学薬学研究部, 助手 (30322308)
YANO Shigetoshi Kumamoto University, Neurosurgery, Assistant, 医学部附属病院, 助手 (60332871)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2004: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2003: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | ischemia / MCA occlusion / vanadate / apoptosis / Akt / ERK |
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| Research Abstract |
Orthovanadate is a competitive inhibitor of protein tyrosine phosphatases. Some of its reported biologic effects are its insulin mimetic property and its activation of phosphoinositide 3-kinase and extracellular-signal regulated kinase(ERK). The authors previously reported its neuroprotective effect on delayed neuronal death of gerbil hippocampal CA1 neurons via Akt and ERK activation after transient forebrain ischemia. In the present study, the neuroprotective effect of postischemic intraperitoneal administration of sodium orthovanadate(2 l/kg of 50-mmol/l sodium orthovanadate in saline) was investigated in rats with transient middle cerebral artery occlusion. Ischemic neuronal injury was evaluated 1 day and 28 days after ischemia. The neuroprotective effect of orthovanadate was significant in the cortex but not the caudate putamen(ischemic core) at both 1 and 28 days after ischemia. In orthovanadate group, the activities of Akt and ERK were maintained after reperfusion ; they were decreased in saline group. Blood glucose level decreased but within normal range.
Regional cerebral blood flow was lower than that of saline group only at 0 hours after reperfusion. These data suggest that orthovanadate has neuroprotective effects in rats with transient middle cerebral artery occlusion and that these effects are mediated by Akt and ERK activation. Furthermore, low blood glucose levels and gradual recovery of regional cerebral blood flow may contribute to neuroprotection.
Next, we injected vanadte after ischemia. The number of Budr/double cortin positive cells decreased remarkably in SVZ. Thus we concluded that vanadate induced progenitor cells after ischemia.
Vanadate may have a potential that induced neuronal regeneration. Further investigation and trial for treatment were needed.
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