| Project/Area Number |
15H04286
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Kyoto University (2017)
University of Miyazaki (2015-2016) |
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Principal Investigator |
Honda Arata 京都大学, 京都市, 特定准教授 (10373367)
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| Co-Investigator(Kenkyū-buntansha) |
下島 圭子 東京女子医科大学, 医学部, 特任助教 (30578935)
山海 直 国立研究開発法人医薬基盤・健康・栄養研究所, 医薬基盤研究所 霊長類医科学研究センター, 主任研究員 (80300937)
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| Co-Investigator(Renkei-kenkyūsha) |
OGURA Atsuo 独立行政法人理化学研究所, バイオリソース研究センター, 室長 (20194524)
MATSUDA Osamu 京都府立医科大学, 医学系研究科, 教授 (00271164)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2017: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Fiscal Year 2016: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2015: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
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| Keywords | Experimental Animals / ES cells / iPS cells / naive / germ cells / chimera / iPS細胞 / ナイーブ変換 / キメラ / ゲノム編集 / 難治性疾患 / ES細胞 / ウサギ / カニクイザル |
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| Outline of Final Research Achievements |
This research project had been aimed for the establishment of heredital desease models using iPS cells and genome editing using several animal species. We achieved naive-like conversion of cynomolgus monkey ES/iPS cells, which can enhances their in vitro differentiation potential. Moreover, following mice and rats, the 3rd true naive-iPS cells were successfully established form an endangered species, Tokudaia osimensis. We produced interspecific chimera and elucidated the sexual plasticity of the germ cells of the endangered species.
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