| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2018: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2017: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2016: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2015: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
|
|---|
| Outline of Final Research Achievements |
The aim of this study is to provide the functional importance of the ADP ribosylation factor 6 (Arf6) pathway in the photoreceptor ribbon synapse. By immunofluorescence and immunoelectron microscopy using a novel anti-BRAG2a antibody, we found that BRAG2a, a guanine nucleotide exchange factor specific for Arf6, colocalized to the plasma membrane of lateral walls and processes of photoreceptor terminals within the synaptic cavity with dystroglycan and dystrophin. Pull-down and immunoprecipitation assays using the retinal lysate demonstrated the complex formation of BRAG2a with the dystrophin and beta-dystroglycan. The present study provides evidence for BRAG2a as a novel component of the photoreceptor dystrophin-associated glycoprotein complex (DGC), suggesting the possible functional involvement of the BRAG2a-Arf6 pathway downstream of the DGC in synaptic organization of photoreceptors.
|
