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Interaction of membrane proteins on asymmetric lipid membrane toward creation of artificial translocon

Research Project

Project/Area Number 15H05493
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Bio-related chemistry
Research InstitutionKanagawa Institute of Industrial Sclence and Technology (2017)
Kanagawa Academy of Science and Technology (2015-2016)

Principal Investigator

Kamiya Koki  地方独立行政法人神奈川県立産業技術総合研究所, 人工細胞膜システムグループ, 研究員(任期有) (70612315)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥24,570,000 (Direct Cost: ¥18,900,000、Indirect Cost: ¥5,670,000)
Fiscal Year 2017: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2016: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2015: ¥13,130,000 (Direct Cost: ¥10,100,000、Indirect Cost: ¥3,030,000)
Keywordsリポソーム / リン脂質非対称膜 / 膜タンパク質 / マイクロ流体デバイス / 人工細胞モデル / 人工細胞 / マイクロデバイス / ベシクル / 無細胞タンパク質合成系
Outline of Final Research Achievements

In this study, I investigated interactions between asymmetric lipid membranes and biological molecules (peptides or membrane proteins). The asymmetric lipid vesicles were generated from a planar lipid bilayer by applying the pulsed jet flow. I achieved three items. Frist, I found that the reconstruction amounts of the membrane proteins into the lipid membranes changed by the lipid compositions, Second, I developed a device for sequentially generating various types of asymmetric lipid vesicles. Third, I developed a well-controlled cell or liposome-trapping device.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (29 results)

All 2018 2017 2016 2015 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Acknowledgement Compliant: 1 results) Presentation (18 results) (of which Int'l Joint Research: 3 results,  Invited: 3 results) Book (2 results) Remarks (5 results)

  • [Journal Article] Well-Controlled Cell-Trapping Systems for Investigating Heterogeneous Cell-Cell Interactions2018

    • Author(s)
      Kamiya Koki、Abe Yuta、Inoue Kosuke、Osaki Toshihisa、Kawano Ryuji、Miki Norihisa、Takeuchi Shoji
    • Journal Title

      Advanced Healthcare Materials

      Volume: 7 Issue: 6 Pages: 1701208-1701208

    • DOI

      10.1002/adhm.201701208

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sequential generation of asymmetric lipid vesicles using a pulsed-jetting method in rotational wells2018

    • Author(s)
      Gotanda Masahide、Kamiya Koki、Osaki Toshihisa、Fujii Satoshi、Misawa Nobuo、Miki Norihisa、Takeuchi Shoji
    • Journal Title

      Sensors and Actuators B: Chemical

      Volume: 261 Pages: 392-397

    • DOI

      10.1016/j.snb.2018.01.149

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Giant liposome formation toward the synthesis of well-defined artificial cells2017

    • Author(s)
      Kamiya Koki、Takeuchi Shoji
    • Journal Title

      Journal of Materials Chemistry B

      Volume: 5 Issue: 30 Pages: 5911-5923

    • DOI

      10.1039/c7tb01322a

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cell-sized asymmetric lipid vesicles facilitate the investigation of asymmetric membranes2016

    • Author(s)
      Koki Kamiya, Ryuji Kawano, Toshihisa Osaki, Kazunari Akiyoshi, and Shoji Takeuchi
    • Journal Title

      Nature Chemistry

      Volume: 8 Issue: 9 Pages: 881-889

    • DOI

      10.1038/nchem.2537

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] NANO-SIZED ASYMMETRIC LIPID VESICLES FOR DRUG CARRIER APPLICATIONS2018

    • Author(s)
      Koki Kamiya, Toshihisa Osaki, Satoshi Fujii, Nobuo Misawa,and Shoji Takeuchi
    • Organizer
      MEMS 2018
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] マイクロデバイスを用いたリポソーム作製2018

    • Author(s)
      神谷厚輝、大崎寿久、藤井聡志、三澤宣雄、竹内昌治
    • Organizer
      第1回分子ロボティクス年次大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 人工細胞膜内の生体分子運動を観察可能とする非対称膜リポソームの連続的作製法の開発2017

    • Author(s)
      五反田真秀、神谷厚輝、大崎寿久、藤井聡志、三澤宣雄、三木則尚、竹内昌治
    • Organizer
      ロボティクス・メカトロニクス講演会 2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] 細胞膜相互作用観察可能なジェット水流印加によるリン脂質非対称膜リポソームの作製2017

    • Author(s)
      神谷厚輝、大崎寿久、川野竜司、竹内昌治
    • Organizer
      化学とマイクロ・ナノシステム学会第35回研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 脂質膜組成交換デバイスによる非対称人工細胞膜間の分子運動観察2017

    • Author(s)
      五反田真秀、神谷厚輝、大崎寿久、藤井聡志、三澤宣雄、三木則尚、竹内昌治
    • Organizer
      化学とマイクロ・ナノシステム学会第35回研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Artificial Cell Assembly Using Bottom-Up Approach2017

    • Author(s)
      Koki Kamiya
    • Organizer
      23rd iCeMS International Symposium“Emerging Science for Unlocking Cell's Secrets”
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] SEQUENTIAL PRODUCTION OF VARIOUS TYPES OF ASYMMETRIC LIPID VESICLES USING PULSE JET FLOW2017

    • Author(s)
      Masahide Gotanda, Koki Kamiya, Toshihisa Osaki, Satoshi Fujii, Nobuo Misawa, Norihisa Miki and Shoji Takeuchi
    • Organizer
      TRANSDUCERS 2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] Reconstitution amount of membrane proteins was controlled by components of asymmetric lipid vesicles2017

    • Author(s)
      Koki Kamiya, Toshihisa Osaki, Ryuji Kawano, Shoji Takeuchi
    • Organizer
      第55回日本生物物理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] FORMATION OF A GIANT LIPID VESICLE CONTAINING TWO TYPES OF LIQUID SOLUTIONS USING A THETA-GLASS CAPILLARY2017

    • Author(s)
      Koki Kamiya, Toshihisa Osaki, Satoshi Fujii, Nobuo Misawa, and Shoji Takeuchi
    • Organizer
      MicroTAS 2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] 非対称人工細胞膜間における生体分子の拡散運動観察ため手法開発2017

    • Author(s)
      五反田真秀、神谷厚輝、大崎寿久、藤井聡志、三澤宣雄、三木則尚、竹内昌治
    • Organizer
      第8回マイクロ・ナノ工学シンポジウム
    • Related Report
      2017 Annual Research Report
  • [Presentation] 細胞膜を模倣したリン脂質非対称膜と生体分子の相互作用観察2016

    • Author(s)
      神谷厚輝、大崎寿久、竹内昌治
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] リン脂質非対称膜リポソームによる膜タンパク質の取り込み挙動の観察2016

    • Author(s)
      神谷厚輝、大崎寿久、竹内昌治
    • Organizer
      「細胞を創る」研究会9.0
    • Place of Presentation
      早稲田大学(東京都新宿区)
    • Year and Date
      2016-11-21
    • Related Report
      2016 Annual Research Report
  • [Presentation] 多種類の非対称膜組成リポソームの連続作製法の開発2016

    • Author(s)
      五反田真秀、神谷厚輝、大崎寿久、藤井聡志、三澤宣雄、三木則尚、竹内昌治
    • Organizer
      「細胞を創る」研究会9.0
    • Place of Presentation
      早稲田大学(東京都新宿区)
    • Year and Date
      2016-11-21
    • Related Report
      2016 Annual Research Report
  • [Presentation] リン脂質非対称膜リポソームへの無細胞発現系で発現させた膜タンパク質の再構成2016

    • Author(s)
      神谷厚輝、大崎寿久、竹内昌治
    • Organizer
      日本化学会第96春季年会
    • Place of Presentation
      同志社大学(京都府)
    • Year and Date
      2016-03-24
    • Related Report
      2015 Annual Research Report
  • [Presentation] 膜タンパク質やリン脂質非対称膜を再構成した人工細胞膜の作製2015

    • Author(s)
      神谷厚輝、竹内昌治
    • Organizer
      「細胞を創る」研究会 8.0
    • Place of Presentation
      大阪大学(大阪府)
    • Year and Date
      2015-11-12
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] OBSERVATION OF LIPID MOTION IN ASYMMETRIC GIANT VESICLES2015

    • Author(s)
      Koki Kamiya, Toshihisa Osaki and Shoji Takeuchi
    • Organizer
      Micro TAS2015
    • Place of Presentation
      韓国慶州
    • Year and Date
      2015-10-25
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Preparation of artificial cell models by bottom-up approach2015

    • Author(s)
      神谷厚輝
    • Organizer
      第53回日本生物物理学会年会
    • Place of Presentation
      金沢大学(石川県金沢市)
    • Year and Date
      2015-09-13
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] リン脂質非対称膜リポソームによる生体分子の挙動観察2015

    • Author(s)
      神谷厚輝、大崎寿久、川野竜司、竹内昌治
    • Organizer
      第25回バイオ・高分子シンポジウム
    • Place of Presentation
      東京工業大学(東京都目黒区)
    • Year and Date
      2015-07-23
    • Related Report
      2015 Annual Research Report
  • [Book] 人工細胞とその応用2017

    • Author(s)
      吉田昭太郎、神谷厚輝、竹内昌治
    • Total Pages
      215
    • Publisher
      シーエムシー出版
    • Related Report
      2016 Annual Research Report
  • [Book] 現代化学2016

    • Author(s)
      藤井聡志、神谷厚輝、竹内昌治
    • Publisher
      東京化学同人
    • Related Report
      2016 Annual Research Report
  • [Remarks] 神奈川県立産業技術総合研究所 人工細胞膜システムグループ

    • URL

      https://www.kanagawa-iri.jp/r_and_d/project_res/labo_intro/takeuchi_project/

    • Related Report
      2017 Annual Research Report
  • [Remarks] 竹内昌治研究室

    • URL

      http://www.hybrid.iis.u-tokyo.ac.jp

    • Related Report
      2017 Annual Research Report
  • [Remarks] 神奈川県立産業技術総合研究所

    • URL

      https://www.kanagawa-iri.jp/

    • Related Report
      2016 Annual Research Report
  • [Remarks] 公益財団法人神奈川科学技術アカデミー

    • URL

      https://www.newkast.or.jp/

    • Related Report
      2015 Annual Research Report
  • [Remarks] 東京大学竹内研究室

    • URL

      http://www.hybrid.iis.u-tokyo.ac.jp/

    • Related Report
      2015 Annual Research Report

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

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