Epigenomic landscapes governed by distinct PRC2s

• Project/Area Number: 15K06942
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Molecular biology
• Research Institution: Osaka University (2017); Hokkaido University (2015-2016)
• Principal Investigator: Nagao Koji 大阪大学, 理学研究科, 准教授 (60426575)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: エピジェネティクス / ヒストン修飾

Outline of Final Research Achievements

Polycomb repressive complex 2 (PRC2) play a key role in silencing developmentally regulated genes to establish and maintain facultative heterochromatin. The core PRC2 complex comprises four components (EZH1/2, SUZ12, EED and RBBP4/7) and methylates histone H3 at Lys 27 through its enzymatic subunit EZH1/2. In addition, five regulatory components have been identified in mammalian PRC2. However, how mammalian PRC2 regulates various genomic sites and collaborates with other chromatin modulators remains unclear. Reconstitution of PRC2 in Sf9 insect cells using intact or truncated components resulted in subunit organization of PRC2.

Research Products

• [Journal Article] Defects in dosage compensation impact global gene regulation in the mouse trophoblast (2017)
  • Author(s): Sakata Yuka、Nagao Koji、Hoki Yuko、Sasaki Hiroyuki、Obuse Chikashi、Sado Takashi
  • Journal Title: Development Volume: 144 Issue: 15 Pages: 2784-2797
  • DOI: 10.1242/dev.149138
  • Peer Reviewed / Open Access
• [Journal Article] Inhibition of RIF1 by SCAI Allows BRCA1-Mediated Repair. (2017)
  • Author(s): Isobe S.Y., Nagao K., Nozaki N., Kimura H., and Obuse C.
  • Journal Title: Cell Rep. Volume: 2 Issue: 2 Pages: 297-307
  • DOI: 10.1016/j.celrep.2017.06.056
  • Peer Reviewed / Open Access
• [Journal Article] Exosomes maintain cellular homeostasis by excreting harmful DNA from cells (2017)
  • Author(s): Takahashi, A., Okada, R., Nagao, K., Kawamata, Y., Hanyu, A., Yoshimoto, S., Takasugi, M., Watanabe, S., Kanemaki, M.T., Obuse, C. & Hara, E.
  • Journal Title: Nature Communications Volume: - Issue: 1 Pages: 15287-15287
  • DOI: 10.1038/ncomms15287
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Compositionally distinct nuclear pore complexes of functionally distinct dimorphic nuclei in ciliate Tetrahymena. (2017)
  • Author(s): Iwamoto M., Osakada H., Mori C., Fukuda Y., Nagao K., Obuse C., Hiraoka Y., and Haraguchi T.
  • Journal Title: J Cell Sci. Volume: 印刷中 Issue: 10 Pages: 1822-1834
  • DOI: 10.1242/jcs.199398
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Usp7-dependent histone H3 deubiquitylation regulates maintenance of DNA methylation. (2017)
  • Author(s): Yamaguchi L, Nishiyama A, Misaki T, Johmura Y, Ueda J, Arita K, Nagao K, Obuse C, Nakanishi M
  • Journal Title: Scientific reports Volume: 7 Issue: 1 Pages: 1-12
  • DOI: 10.1038/s41598-017-00136-5
  • Peer Reviewed / Open Access
• [Journal Article] CK2 phospho-independent assembly of the Tel2-associated stress-signaling complexes in Schizosaccharomyces pombe.pendent (2017)
  • Author(s): Inoue, H., Sugimoto, S., Takeshita, Y., Takeuchi, M., Hatanaka, M., Nagao, K., Hayashi, T., Kokubu, A., Yanagida, M., and Kanoh, J..,
  • Journal Title: Genes Cells Volume: 22 Issue: 1 Pages: 59-70
  • DOI: 10.1111/gtc.12454
  • Peer Reviewed / Open Access
• [Journal Article] Histone H3K36 trimethylation is essential for multiple silencing mechanism in fission yeast. (2016)
  • Author(s): Shota Suzuki, Hiroaki Kato, Yutaka Suzuki, Yuji Chikashige, Yasushi Hiraoka, Hiroshi Kimura, Koji Nagao, Chikashi Obuse, Shinya Takahata and Yota Murakami
  • Journal Title: Nucleic Acids Research Volume: 44 (9) Issue: 9 Pages: 4147-4162
  • DOI: 10.1093/nar/gkw008
  • NAID: 120005819302
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Histone H4 lysine 20 acetylation is associated with gene repression in human cells. (2016)
  • Author(s): Kaimori, J. Y., Maehara, K., Hayashi-Takanaka, Y., Harada, A., Fukuda, M., Yamamoto, S., Ichimaru, N., Umehara, T., Yokoyama, S., Matsuda, R., Ikura, T., Nagao, K., Obuse, C., Nozaki, N., Takahara, S., Takao, T., Ohkawa, Y., Kimura, H., and Isaka, Y.
  • Journal Title: Sci Rep. Volume: 6 Issue: 1 Pages: 24318-24318
  • DOI: 10.1038/srep24318
  • NAID: 120005770760
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Clinical, muscle pathological, and genetic features of Japanese facioscapulohumeral muscular dystrophy 2 (FSHD2) patients with SMCHD1 mutations. (2016)
  • Author(s): Hamanaka, K., Goto, K., Arai, M., Nagao, K., Obuse, C., Noguchi, S., Hayashi, Y. K., Mitsuhashi, S., and Nishino, I.
  • Journal Title: Neuromuscul Disord. Volume: 26 Issue: 4-5 Pages: 300-308
  • DOI: 10.1016/j.nmd.2016.03.001
  • Peer Reviewed / Open Access
• [Presentation] Smchd1-Hbix1依存的な不活性化 X染色体の区画化 (2017)
  • Author(s): 長尾恒治、榊原祐樹、柴田幸子、野澤竜介、元田裕佳里、坂口武久、木村宏、佐渡敬、小布施力史
  • Organizer: 2017年度生命科学系学会合同年次大会（ConBio2017）
• [Presentation] Smchd1-Hbix1依存的な不活性化X染色体の区画化 (2017)
  • Author(s): 長尾恒治
  • Organizer: 第5回 X染色体研究会
• [Presentation] 次世代シーケンサーを使ったX染色体のアリル特異的な解析 (2016)
  • Author(s): 長尾恒治
  • Organizer: 第4回 X染色体研究会
  • Place of Presentation: 鳥取大学医学部臨床講義棟（鳥取県米子市）
  • Year and Date: 2016-03-27
• [Presentation] アリル特異的ChIP-seq法によるマウス不活性化X染色体のクロマチン動態の解析 (2016)
  • Author(s): 長尾恒治、榊原祐樹、柴田幸子、野澤竜介、坂口武久、木村宏、佐渡敬、小布施力史
  • Organizer: 第１回北大・部局横断シンポジウム「生体防御 システムとその破綻」
  • Place of Presentation: 北海道大学医学部学友会館（北海道札幌市）
  • Year and Date: 2016-03-07
• [Presentation] アリル特異的ChIP/RNA-seq法によるマウス不活性化X染色体のクロマチン動態の解析 (2016)
  • Author(s): 長尾恒治、榊原祐樹、柴田幸子、野澤竜介、坂口武久、木村宏、佐渡敬、小布施力史
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜 (神奈川県・横浜市)
• [Presentation] ChIP-seq法によるマウス不活性化X染色体のクロマチン動態の解析 (2016)
  • Author(s): 長尾恒治、榊原祐樹、柴田幸子、野澤竜介、坂口武久、木村宏、佐渡敬、小布施力史
  • Organizer: クロマチン動構造 若手ワークショップ
  • Place of Presentation: ルスツリゾート (北海道・虻田郡留寿都村)
• [Presentation] アリル特異的ChIP-seq法によるマウス不活性化X染色体のクロマチン動態の解析 (2015)
  • Author(s): 長尾恒治、榊原祐樹、柴田幸子、野澤竜介、坂口武久、木村宏、佐渡敬、小布施力史
  • Organizer: 第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会
  • Place of Presentation: 神戸ポートピアホテル（兵庫県神戸市）
  • Year and Date: 2015-12-01
• [Presentation] Evolutionary conserved chromatin states of the inactive X chromosome between human and mouse (2015)
  • Author(s): 長尾恒治、柴田幸子、野澤竜介、木村宏、佐渡敬、小布施力史
  • Organizer: 第40回内藤コンファレンス
  • Place of Presentation: シャトレーゼ ガトーキングダム サッポロ（北海道札幌市）
  • Year and Date: 2015-09-16
  • Int'l Joint Research
• [Presentation] 質量分析器と次世代シーケンサーを駆使したクロマチン研究 (2015)
  • Author(s): 長尾恒治
  • Organizer: 日本内分泌学会 第33回内分泌代謝学サマーセミナー
  • Place of Presentation: 柳川藩主立花邸 御花（福岡県柳川市）
  • Year and Date: 2015-07-09
  • Invited