Mechanisms of acquisition of organic phosphate by arbuscular mycorrhizal fungi

• Project/Area Number: 15K07332
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant nutrition/Soil science
• Research Institution: Yamagata University
• Principal Investigator: Tawaraya Keitaro 山形大学, 農学部, 教授 (70179919)
• Project Period (FY): 2015-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 有機態リン酸 / アーバスキュラー菌根菌 / 酸性ホスファターゼ / 外生菌糸 / 菌種間差 / ホスファターゼ / 菌根菌 / 菌糸 / リン酸 / ネギ

Outline of Final Research Achievements

Extraradical hyphae of arbuscular mycorrhizal (AM) fungi have acid phosphatase but its exudation is unknow. Soil solution was collected from hyphal compartment inoculated with AM fungus Rhizophagus clarus. ACP activity of 187 kDa was detected from soil solution of inoculated plant. Acid phosphatase activity in hyphal compartment of Linum usitatissimum was higher at low P level than that at high P level. These results suggest that AM fungi R. clarus exudes ACP and its exudation increased under P deficient condition.

Academic Significance and Societal Importance of the Research Achievements

リン酸資源枯渇へ対応するためには農耕地における有機態リン酸を利用する必要がある。土壌中の外生菌糸から酸性ホスファターゼを浸出するアーバスキュラー菌根菌を作物に接種して利用することができれば、農耕地における有機態リン酸の利用が向上し、リン酸資源を国内で持続的に循環させることができる。

Research Products

• [Journal Article] Release of acid phosphatase from extraradical hyphae of arbuscular mycorrhizal fungus Rhizophagus clarus (2015)
  • Author(s): Takumi SATO, Tatsuhiro EZAWA, Weiguo CHENG, Keitaro TAWARAYA
  • Journal Title: Soil Science and Plant Nutrition Volume: 61 Issue: 2 Pages: 269-279
  • DOI: 10.1080/00380768.2014.993298
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] アーバスキュラー菌根菌による土壌中の有機態リン酸の利用 (2018)
  • Author(s): 俵谷圭太郎、佐藤匠
  • Organizer: 日本土壌肥料学会２０１８年度神奈川大会
  • Invited
• [Presentation] アーバスキュラー菌根菌が浸出する酸性ホスファターゼ活性の菌種間差 (2018)
  • Author(s): 佐藤匠、江沢辰広、程為国、俵谷圭太郎
  • Organizer: 日本土壌肥料学会２０１８年度神奈川大会
• [Presentation] ow-phosphorus conditions increase release of acid phosphatase from extraradical hyphae of Rhizophagus clarus (2017)
  • Author(s): Hachiya. S, Inamura. N, Sato. T, T. Ezawa, W. Cheng, and K. Tawaraya
  • Organizer: 9th International conference of mycorrhiza
  • Int'l Joint Research
• [Presentation] アーバスキュラー菌根菌 Rhizophagus clarus の外生菌糸から浸出される酸性ホスファターゼ活性の低リン酸応答 (2017)
  • Author(s): 蜂矢志保実、稲村希、佐藤匠、江沢辰広、程為国、俵谷圭太郎
  • Organizer: 日本土壌肥料学会仙台大会
• [Presentation] Release of acid phosphatase from extraradical hyphae of arbuscular mycorrhizal fungus Rhizophagus clarus (2016)
  • Author(s): 俵谷圭太郎
  • Organizer: Organic Phosphorus Workshop 2016
  • Place of Presentation: Low Wood Bay Hotel, Lake District
  • Year and Date: 2016-09-05
  • Int'l Joint Research
• [Presentation] 低リン条件におけるアーバスキュラー菌根菌Rhizophagus clarusの外生菌糸による酸性ホスファターゼの浸出 (2015)
  • Author(s): 佐藤 匠、程 為国、江沢辰広、俵谷圭太郎
  • Organizer: 日本土壌肥料学会２０１５年度京都大会
  • Place of Presentation: 京都市、京都大学
  • Year and Date: 2015-09-09