Early closure of alveolar cleft by tissue regeneration using umbilical stem cells
| Project/Area Number |
15K15759
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Orthodontics/Pediatric dentistry
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| Research Institution | Meikai University |
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Principal Investigator |
Suda Naoto 明海大学, 歯学部, 教授 (90302885)
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| Co-Investigator(Kenkyū-buntansha) |
真野 樹子 明海大学, 歯学部, 准教授 (00333005)
時岡 一幸 埼玉医科大学, 医学部, 准教授 (70332616)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | 口蓋裂 / 再生 / 骨 / 臍帯幹細胞 / 顎裂 |
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| Outline of Final Research Achievements |
Human umbilical cells (UC-EZ) were derived from enzymatic digestion. Using magnetic-activated cell sorting (MACS), CD146-positive mesenchymal stem cells (US-MACS) were isolated. Two kinds of cells were positive for stem cell markers. UC-EZ were positive for Nanog and Oct3/4 but negative for Sox2. Two cells formed alizarin red-positive calcified matrix and oil red O positive lipid droplets. UC-MACS were positive for all stem cell markers and undifferentiated maintenance-related genes. UC-MACS showed high capacity to differentiate toward osteoblasts and adipocytes.
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Report
(4 results)
Research Products
(19 results)
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[Journal Article] Lectin-like oxidized low-density lipoprotein receptor-1 abrogation causes resistance to inflammatory bone destruction in mice, despite promoting osteoclastogenesis in the steady state.2015
Author(s)
Nakayachi M, Ito J, Hayashida C, Ohyama Y, Kakino A, Okayasu M, Sato T, Ogasawara T, Kaneda T, Suda N, Sawamura T, Hakeda Y
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Journal Title
Bone. 75:170-182
Volume: 75
Pages: 170-182
DOI
Related Report
Peer Reviewed / Open Access
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