Early closure of alveolar cleft by tissue regeneration using umbilical stem cells

• Project/Area Number: 15K15759
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Orthodontics/Pediatric dentistry
• Research Institution: Meikai University
• Principal Investigator: Suda Naoto 明海大学, 歯学部, 教授 (90302885)
• Co-Investigator(Kenkyū-buntansha): 真野 樹子 明海大学, 歯学部, 准教授 (00333005) ; 時岡 一幸 埼玉医科大学, 医学部, 准教授 (70332616)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 口蓋裂 / 再生 / 骨 / 臍帯幹細胞 / 顎裂

Outline of Final Research Achievements

Human umbilical cells (UC-EZ) were derived from enzymatic digestion. Using magnetic-activated cell sorting (MACS), CD146-positive mesenchymal stem cells (US-MACS) were isolated. Two kinds of cells were positive for stem cell markers. UC-EZ were positive for Nanog and Oct3/4 but negative for Sox2. Two cells formed alizarin red-positive calcified matrix and oil red O positive lipid droplets. UC-MACS were positive for all stem cell markers and undifferentiated maintenance-related genes. UC-MACS showed high capacity to differentiate toward osteoblasts and adipocytes.

Research Products

• [Journal Article] Effects of FKBP12 and type II BMP receptors on signal transduction by ALK2 activating mutations associated with genetic disorders (2018)
  • Author(s): Machiya Aiko、Tsukamoto Sho、Ohte Satoshi、Kuratani Mai、Fujimoto Mai、Kumagai Keigo、Osawa Kenji、Suda Naoto、Bullock Alex N.、Katagiri Takenobu
  • Journal Title: Bone Volume: 111 Pages: 101-108
  • DOI: 10.1016/j.bone.2018.03.015
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Orthodontic treatment-induced temporal alteration of jaw-opening reflex excitability. (2017)
  • Author(s): Sasaki A, Hasegawa N, Adachi K, Sakagami H, Suda N
  • Journal Title: J Neurophysiol Volume: 118 Issue: 4 Pages: 2289-2295
  • DOI: 10.1152/jn.00379.2017
  • Peer Reviewed
• [Journal Article] Intracortical signal processing of periodontal ligament sensations in rat. (2017)
  • Author(s): Minoda A, Mizoguchi N, Kobayashi M, Suda N, Muramoto K
  • Journal Title: Neuroscience Volume: 355 Pages: 212-224
  • DOI: 10.1016/j.neuroscience.2017.04.045
  • Peer Reviewed
• [Journal Article] Heterogeneous human periodontal ligament-committed progenitor and stem cell populations exhibit a unique cementogenic property under in vitro and in vivo conditions (2017)
  • Author(s): Shinagawa-Ohama R, Mochizuki M, Tamaki Y, Suda N, Nakahara T
  • Journal Title: Stem Cells and Development Volume: 26 Issue: 9 Pages: 632-645
  • DOI: 10.1089/scd.2016.0330
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Growth of maxillo-facial region and related anomalies. (2017)
  • Author(s): Suda N
  • Journal Title: Clin Calcium Volume: 27 Pages: 1357-1362
• [Journal Article] 舌小帯切除術を施行した舌小帯強直症3例における舌運動と舌圧の評価 (2017)
  • Author(s): 森脇由華、大塚雄一郎、須田直人
  • Journal Title: 明海歯科医学 Volume: 46 Pages: 22-33
  • NAID: 40021106298
  • Peer Reviewed / Open Access
• [Journal Article] ニッケルアレルギーに配慮し上顎歯列の狭窄と骨格性開咬に対しSARMEと上下顎同時移動術を施行した1例 (2017)
  • Author(s): 権田秋華、真野樹子、長谷川紘也、須田直人
  • Journal Title: Orthodontic Wave Volume: 76 Pages: 1-9
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 矯正歯科用レジン添加型グラスアイオノマーセメントを用いたメタルブラケットとエナメル質の接着強さ ─サーマルサイクルと粉液比が接着強さに及ぼす影響─ (2016)
  • Author(s): 峯岸至保 日比野 靖 中嶌 裕 須田直人
  • Journal Title: 接着歯学 Volume: 34 Pages: 68-81
  • Open Access / Int'l Joint Research
• [Journal Article] Lectin-like oxidized low-density lipoprotein receptor-1 abrogation causes resistance to inflammatory bone destruction in mice, despite promoting osteoclastogenesis in the steady state. (2015)
  • Author(s): Nakayachi M, Ito J, Hayashida C, Ohyama Y, Kakino A, Okayasu M, Sato T, Ogasawara T, Kaneda T, Suda N, Sawamura T, Hakeda Y
  • Journal Title: Bone. 75:170-182 Volume: 75 Pages: 170-182
  • DOI: 10.1016/j.bone.2015.02.025
  • Peer Reviewed / Open Access
• [Journal Article] Evaluation of mechanical properties of esthetic brackets. (2015)
  • Author(s): Matsui S, Umezaki E, Komazawa D, Otsuka Y, Suda N
  • Journal Title: J Dent Biomech Volume: 26 Issue: 0
  • DOI: 10.1177/1758736015574401
  • Peer Reviewed / Open Access
• [Journal Article] Mutant activin-like kinase 2 in fibrodysplasia ossificans progressiva are activated via T203 by BMP type II receptors. (2015)
  • Author(s): Fujimoto M, Ohte S, Osawa K, Miyamoto A, Tsukamoto S, Mizuta T, Kokabu S, Suda N, Katagiri T.
  • Journal Title: Mol Endocrinol Volume: 29 Pages: 140-152
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A case of unilateral cleft lip and palate with postoperative skeletal stability after maxillary distraction osteogenesis (2015)
  • Author(s): Sasaki A, Tanaka E, Mano M, Suda N
  • Journal Title: Jpn J Jaw Deform Volume: 25 Pages: 223-233
  • NAID: 130005098032
  • Peer Reviewed
• [Journal Article] 精度の高い歯列画像を有する三次元顎顔面頭蓋画像の再構成 (2015)
  • Author(s): 三條恵介、大塚雄一郎、須田直人ら
  • Journal Title: 日顎変誌 Volume: 25 Pages: 207-2017
  • NAID: 130005098036
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Occlusal management of CLP children in non-metropolitan Japanese area (2017)
  • Author(s): Suda N
  • Organizer: 13th International Cleft Congres
• [Presentation] Maxillary canine inclination after gingivoperiosteoplasty in patients with unilateral cleft lip and palate (UCLP) (2017)
  • Author(s): Suda N
  • Organizer: 13th International Cleft Congres
• [Book] Molecular mechanisms for activation of mutant activin receptor-like kinase 2 in fibrodysplasia ossificans progressiva. (2017)
  • Author(s): Fujimoto M, Suda N, Katagiri T
  • Total Pages: 10
  • Publisher: Journal of Oral Biosciences
• [Book] マルファン症候群ガイドブック (2017)
  • Author(s): 須田直人
  • Total Pages: 12
  • Publisher: マルファンネットワークジャパン
• [Book] 新・歯科衛生士教育マニュアル 歯科矯正学 (2015)
  • Author(s): 須田直人
  • Total Pages: 171
  • Publisher: クインテッセンス出版
• [Book] Advances in Genetics Research, Vol 14 (2015)
  • Author(s): Suda N
  • Total Pages: 30
  • Publisher: Nova Science Publishers