Analysis for transcriptional regulation mechanism of retrotransposon

• Project/Area Number: 15K18578
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Genetics/Chromosome dynamics
• Research Institution: Okayama University
• Principal Investigator: Ikeda Yoko 岡山大学, 資源植物科学研究所, 特任助教 (80467688)
• Research Collaborator: MATHIEU Olivier クレルモンフェラン大学, Principal investigator
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: トランスポゾン / エピジェネティクス / サイレンシング

Outline of Final Research Achievements

To reveal the transcriptional regulation mechanism of retrotransposons, we carried out a screen of the mutants affected in the silencing at a transgene. We identified Arabidopsis gene affecting heterochromatin-associated silencing, KUMONOSU (KUN). KUN encodes a plant-specific retrotransposon related plant mobile domain (PMD), and affects silencing pathway by impaired condensation of pericentromeric heterochromatin. KUN acts for silencing independent of DNA methylation and known chromatin modifications.

Research Products

• [Int'l Joint Research] Universite Clermont Auvergne/CNRS/Inserm(フランス)
• [Int'l Joint Research] Max planck institute/Universitat Hamburg(ドイツ)
• [Int'l Joint Research] Universite Clermont Auvergne/CNRS/Inserm(フランス)
• [Int'l Joint Research] Max Planck Institute/Universitat Hamburg(ドイツ)
• [Int'l Joint Research] CNRS, UMR 6293/INSERM U1103(Olivier Mathieu)
• [Journal Article] Arabidopsis proteins with a transposon-related domain act in gene silencing. (2017)
  • Author(s): Ikeda, Y., Pelissier, T., Bourguet, P., Becker, C., Pouch-Pelissier, M. N., Pogorelcnik, R., Weingartner, M., Weigel, D., Deragon, J. M. and Mathieu, O.
  • Journal Title: Nature Communications Volume: 8 Issue: 1 Pages: 15122-15122
  • DOI: 10.1038/ncomms15122
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Comprehensive quantification and genome survey reveal the presence of novel phytohormone action modes in red seaweeds (2016)
  • Author(s): Mikami, K., Mori, I.C., Matsuura, T., Ikeda, Y., Kojima, M., Sakakibara, H., Hirayama, T.
  • Journal Title: J. Applied Phycology Volume: 未定 Issue: 4 Pages: 1-10
  • DOI: 10.1007/s10811-015-0759-2
  • NAID: 120006329010
  • Peer Reviewed
• [Journal Article] Epigenome confrontation triggers immediate reprogramming of DNA methylation and transposon silencing in Arabidopsis thaliana F1 epihybrids. (2016)
  • Author(s): Rigal M, Becker C, Pélissier T, Pogorelcnik R, Devos J, Ikeda Y, Weigel D, Mathieu O.
  • Journal Title: Proc. Natl. Acad. Sci. USA. Volume: 113 Issue: 14 Pages: 2083-2092
  • DOI: 10.1073/pnas.1600672113
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Light inhibition of shoot regeneration is regulated by endogenous abscisic acid level in calli derived from immature barley embryos. (2015)
  • Author(s): Rikiishi K., Matsuura T., Ikeda Y., Maekawa M.
  • Journal Title: PLOS ONE Volume: オンライン雑誌のためなし Issue: 12 Pages: e0145242-e0145242
  • DOI: 10.1371/journal.pone.0145242
  • Peer Reviewed
• [Presentation] KUMONOSU protein harboring a transposon-related domain act in gene silencing in Arabidopsis (2017)
  • Author(s): 池田陽子 他
  • Organizer: 第58回日本植物生理学会年会
  • Place of Presentation: 鹿児島大学
  • Year and Date: 2017-03-16
• [Presentation] Epigenetic response to various environments in plants and its mechanism. (2017)
  • Author(s): Yoko Ikeda
  • Organizer: 第33回資源植物科学シンポジウム・第9回植物ストレス科学研究シンポジウム
  • Place of Presentation: 倉敷
  • Year and Date: 2017-03-06
  • Int'l Joint Research / Invited
• [Presentation] トランスポゾン関連ドメインをもつ新規遺伝子サイレンシング因子の同定 (2017)
  • Author(s): Ikeda, Y., Pelissier, T., Bourguet, P., Becker, C., Pouch-Pelissier, M. N., Pogorelcnik, R., Weingartner, M., Weigel, D., Deragon, J. M. and Mathieu, O.
  • Organizer: 日本植物学会第81回大会
• [Presentation] トランスポゾン関連ドメインをもつ新規遺伝子サイレンシング因子の同定 (2017)
  • Author(s): Ikeda, Y., Pelissier, T., Bourguet, P., Becker, C., Pouch-Pelissier, M. N., Pogorelcnik, R., Weingartner, M., Weigel, D., Deragon, J. M. and Mathieu, O.
  • Organizer: 日本遺伝学会第89回大会
• [Presentation] The role of plant mobile domain family protein in gene silencing. (2017)
  • Author(s): Ikeda, Y.
  • Organizer: 国立遺伝学研究所 研究集会
  • Invited
• [Presentation] DNA methylation is necessary for maintaining differentiated cells in Marchantia polymorpha. (2016)
  • Author(s): Yoko Ikeda 他
  • Organizer: The EMBO Workshop: New model systems for early land plant evolution.
  • Place of Presentation: Vienna, Austria
  • Year and Date: 2016-06-22
  • Int'l Joint Research
• [Presentation] ゼニゴケにおけるDNAメチル化の機能解析 (2016)
  • Author(s): 池田陽子, 西浜竜一, 山岡尚平, 河内孝之, 平山隆志
  • Organizer: 日本植物生理学会第５７回年会
  • Place of Presentation: 岩手大学
  • Year and Date: 2016-03-18
• [Presentation] シロイヌナズナKUMONOSU遺伝子はヘテロクロマチンサイレンシングに関与する. (2015)
  • Author(s): 池田陽子・Olivier Mathieu
  • Organizer: 第38回日本分子生物学会年会ワークショップ「植物エピゲノム研究の最前線」
  • Place of Presentation: 神戸
  • Year and Date: 2015-12-01
  • Invited
• [Presentation] Identification of a new heterochromatic silencing related factor in Arabidopsis. (2015)
  • Author(s): Ikeda, Y.
  • Organizer: 国立遺伝学研究所 研究集会
  • Place of Presentation: 三島
  • Year and Date: 2015-07-29
• [Book] Chapter 2. The role of DNA methylation in transposable element silencing and genomic imprinting. Nuclear Functions in Plant Transcription, Signaling and Development. (2015)
  • Author(s): Ikeda, Y., Nishimura, T.
  • Total Pages: 18
  • Publisher: Springer