Project/Area Number |
15K19658
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Embryonic/Neonatal medicine
|
Research Institution | Fukushima Medical University |
Principal Investigator |
Ogasawara Kei 福島県立医科大学, 医学部, 助教 (00510348)
|
Research Collaborator |
FUJIMORI Keiya
HASHIMOTO Koichi
MIYAZAKI Kyohei
SATO Maki
GO Hayato
|
Project Period (FY) |
2015-04-01 – 2018-03-31
|
Project Status |
Completed (Fiscal Year 2017)
|
Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
Keywords | 血管透過性 / グルココルチコイド受容体 / SNP / ハイドロコルチゾン / HUVEC / グルココルチコイド |
Outline of Final Research Achievements |
1)Solute permeability test was conducted to examine the cell permeability after hydrocortisone. Although our hypothesis was made that the permeability of the cell decreased after administration of hydrocortisone and transepithelial electrical resistance (TER) increased. The result was different from the hypothesis. We have also confirmed that the value of TER does not change when hydrocortisone is administered after adding GR antagonist (RU 486) to HUVEC. 2) Single nucleotide polymorphisms (SNP) analysis of GR from HUVEC was performed in 7 cases. In the SNP analysis of the Bcl1 gene of GR, there were 6 cases in C/C, 0 cases in C/G, and 1 case in G/G. We will analyze SNPs of other HUVECs in the future as well as to investigate the influence of SNP on the value of TER after hydrocortisone administration.
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