Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Outline of Final Research Achievements |
We investigated the role of gene mutation in oncogenic process using the genome editing CRISPR/Cas system of head and neck squamous cell carcinoma. We established the knock out cells of p53 and PIK3CA gene at HNSCC cell lines, Det562, Fadu and UMSCC47 using the CRISPR/Cas system. Moreover, at UMSCC47, HPV16 positive cell line, we also knocked out E6 and E7 genes which play the important role in oncogenic process. P53 gene knock in cell lines are made at Det562, but Established PIK3CA gene knock in cell lines was unsuccessful, because genome editing of PIK3CA gene leads the fatal damages on cell lines. These data indicated that CRISPR/Cas system is suitable for genome editing of HNSCC cell lines and p53, PIK3CA, E6 and E7 genes are important for oncogenic process.
|