The development for the stable high expression of numerous transgenes.

• Project/Area Number: 16H05401
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General surgery
• Research Institution: Osaka University
• Principal Investigator: Miyagawa Shuji 大阪大学, 医学系研究科, 准教授 (90273648)
• Co-Investigator(Kenkyū-buntansha): 長嶋 比呂志 明治大学, 農学部, 専任教授 (50318664)
• Research Collaborator: Ikawa Masahito
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000); Fiscal Year 2018: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2017: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2016: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
• Keywords: special construct / pig ROSA26 locus / CD200 / CL-SP-D / CD31 / PQA-18 / Xenotransplantation / Gene-modified pigs / Hybrid gene / Complement / Macrophage / Innate Immunity / バイオ人工膵島 / 異種移植 / 新奇遺伝子開発 / 補体制御因子 / Hybrid / Hybid / 抗凝固因子 / バイオテクノロジー / 移植・再生医療 / 生物・生体工学 / 応用動物 / 遺伝子

Outline of Final Research Achievements

We have produced special construct genes. The expression of NCTDM was not efficient, then separated to C1DM & TM. HLA-Ev(147)-2A-beta2m was also produced. In addition, we produced PI-anchored CD47, PI-anchored TFPI. A project of pig ROSA26 locus was started. We demonstrated the targeted knock-in of a gene cassette in this locus. Three guide RNA-sequences were designed, using CRISPR/Cas9 system, and the cleavage efficiency of each plasmid was assessed.
We studied novel strategies for suppressing macrophages and neutrophil-mediated xenogeneic rejection using pig ROSA26 locus. Moreover, we focused on the effect of new immunosuppressants on monocyte/macrophages, such as a PQA-18. PQA-18 showed the potential function on macrophage-mediated cytotoxicity, as an immunosuppressant for xenotransplantation.

Academic Significance and Societal Importance of the Research Achievements

今回の我々の研究は、国際的にも独自性が高いものであり、遺伝子改変ブタ作りに有効である。また、ROSA26の座に遺伝子をknockinさせる方法を確立させる事も有効である。更に、新たに異種移植に有効な分子を数々検定し、また免疫抑制剤を研究する事も、この分野に非常に有効と考えられる。
我々がこれらの遺伝子改変ブタを作出できなければ、外国の遺伝子改変ブタを使う事になる。これに伴い、関連する分野も含めて日本のこの分野での立ち遅れが必至となる。逆に、成功すれば、アジアを中心に、アメリカ、欧州への輸出が可能になり、これまで人の死を前提としていた移植医療を根本的に変える事になる。

Research Products

• [Journal Article] The novel immunosuppressant prenylated quinolinecarboxylic acid-18 (PQA-18) suppresses macrophage differentiation and cytotoxicity in xenotransplantation (2019)
  • Author(s): Lo Pei-Chi、Maeda Akira、Kodama Tasuku、Takakura Chihiro、Yoneyama Tomohisa、Sakai Rieko、Noguchi Yuki、Matsuura Rei、Eguchi Hiroshi、Matsunami Katsuyoshi、Okuyama Hiroomi、Miyagawa Shuji
  • Journal Title: Immunobiology Volume: - Issue: 4 Pages: 575-584
  • DOI: 10.1016/j.imbio.2019.04.003
  • Peer Reviewed / Open Access
• [Journal Article] Human CD31 on porcine cells suppress xenogeneic neutrophil-mediated cytotoxicity via the inhibition of NETosis (2018)
  • Author(s): Wang Han-Tang、Maeda Akira、Sakai Rieko、Lo Pei-Chi、Takakura Chihiro、Jiaravuthisan Patmika、Mod Shabri Afifah、Matsuura Rei、Kodama Tasuku、Hiwatashi Shohei、Eguchi Hiroshi、Okuyama Hiroomi、Miyagawa Shuji
  • Journal Title: Xenotransplantation Volume: 25 Issue: 5
  • DOI: 10.1111/xen.12396
  • Peer Reviewed / Open Access
• [Journal Article] Immunological Response of Pigs to Human Cells, Including Issues Such as the Production of Natural Antibodies in Newborns (2018)
  • Author(s): Sakai R.、Watanabe M.、Maeda A.、Lo P.C.、Wang H.T.、Matsuura R.、Kodama T.、Eguchi H.、Ueno T.、Inoue R.、Nagashima H.、Okuyama H.、Miyagawa S.
  • Journal Title: Transplantation Proceedings Volume: 50 Issue: 9 Pages: 2839-2841
  • DOI: 10.1016/j.transproceed.2018.03.074
  • Peer Reviewed / Open Access
• [Journal Article] A membrane-type surfactant protein D (SP-D) suppresses macrophage-mediated cytotoxicity in swine endothelial cells (2018)
  • Author(s): Jiaravuthisan Patmika、Maeda Akira、Takakura Chihiro、Wang Han-Tang、Sakai Rieko、Shabri Afifah Mohd、Lo Pei-Chi、Matsuura Rei、Kodama Tasuku、Eguchi Hiroshi、Okuyama Hiroomi、Miyagawa Shuji
  • Journal Title: Transplant Immunol Volume: - Pages: 30132-30136
  • DOI: 10.1016/j.trim.2018.02.003
  • Peer Reviewed
• [Journal Article] Human CD31 on porcine cells suppress xenogeneic neutrophil-mediated cytotoxicity via the inhibition of NETosis (2018)
  • Author(s): Han-Tang Wang, Akira Maeda, Rieko Sakai, Pei-Chi Lo, Chihiro Takakura, Patmika Jiaravuthisan, Afifah Mod Shabri, Rei Matsuura, Tasuku Kodama, Shohei Hiwatashi, Hiroshi Eguchi, Hiroomi Okuyama and Shuji Miyagawa
  • Journal Title: Xenotransplantation Volume: 印刷中
  • Peer Reviewed
• [Journal Article] REMINISCENCES OF XENOTRANSPLANTATION STUDIES (2018)
  • Author(s): Shuji Miyagawa
  • Journal Title: Reminiscences of xeno pioneers Volume: -
• [Journal Article] The State of Xenotransplantation (2017)
  • Author(s): Shuji Miyagawa, Akira Maeda, Hiroshi Eguchi, Rieko Sakai, Pei-Chi Lo, Hantang Wang, Chihiro Takakura, Tasuku Komama, Rei Matsuura and Hiroomi Okuyama
  • Journal Title: Xenotransplantation - New Insights Volume: - Pages: 3-10
  • DOI: 10.5772/intechopen.69563
  • ISBN: 9789535133551, 9789535133568
  • Open Access
• [Journal Article] Human CD200 suppresses macrophage-mediated xenogeneic cytotoxicity and phagocytosis (2017)
  • Author(s): Sakai Rieko、Maeda Akira、Choi Thuy-Vy、Lo Pei-Chi、Jiaravuthisan Patmika、Shabri Afifah Mod、Wang Han-Tang、Matsuura Rei、Kodama Tasuku、Eguchi Hiroshi、Okuyama Hiroomi、Miyagawa Shuji
  • Journal Title: Surg Today Volume: 48 Issue: 1 Pages: 119-126
  • DOI: 10.1007/s00595-017-1546-2
  • Peer Reviewed
• [Journal Article] Studies of innate immune systems against human cells (2017)
  • Author(s): Rieko Sakai, Etsuko Kitano, Akira Maeda, et al
  • Journal Title: Transplant Immunol Volume: 40 Pages: 66-71
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Depression of Complement Regulatory Factors in Rat and Human Renal Grafts is Associated with the Progress of Acute T-cell Mediated Rejection (2016)
  • Author(s): Kazuaki Yamanaka, Yoichi Kakuta, Shuji Miyagawa et al.
  • Journal Title: PLoS One Volume: 11(2)
  • Peer Reviewed / Open Access
• [Journal Article] Human HLA-Ev (147) Expression in Transgenic Animals (2016)
  • Author(s): Matsuura R, Maeda A, Sakai R, et al.
  • Journal Title: Transplant Proc Volume: 48 Pages: 1323-5
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Knockout of Cytidine Monophospho-N-Acetylneuraminic Acid (CMP-NeuAc) Hydroxylase From Porcine Endothelial Cells by a CRISPR System (2016)
  • Author(s): Sakai R, Esaki Y, Hasuwa H, et al
  • Journal Title: Transplant Proc Volume: 48 Pages: 1320-2
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Supplemental Analysis for N-linked Sugars in Adult Pig Islets (2016)
  • Author(s): Eguchi H, Kawamura T, Kashiyama N, et al
  • Journal Title: Transplant Proc Volume: 48 Pages: 1302-3
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] HLA-G1, but Not HLA-G3, Suppresses Human Monocyte/Macrophage-mediated Swine Endothelial Cell Lysis (2016)
  • Author(s): Eguchi H, Maeda A, Lo PC, et al
  • Journal Title: Transplant Proc Volume: 48 Pages: 1285-7
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Studies of Pig Complement: Measurement of Pig CH50, ACH50, and Components (2016)
  • Author(s): Sakai R, Kitano E, Hatanaka M, et al
  • Journal Title: Transplant Proc Volume: 48 Pages: 1282-4
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Expression of a Synthetic Gene of CTDM by Transgenic Animals (2016)
  • Author(s): Sakai R, Maeda A, Matsuura R, et al
  • Journal Title: Transplant Proc Volume: 48 Pages: 1279-81
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] バイオ人工臓器・細胞の開発 (2018)
  • Author(s): 宮川周士、前田晃、奥山宏臣、長嶋比呂志
  • Organizer: 第63回日本透析医学会学術集会・総会
  • Invited
• [Presentation] ヒトCD31(PECAM1)による好中球誘導異種移植拒絶反応の抑制効果の検討Human CD31 (PECAM1)suppresses the neutrophil-mediated xenogeneic rejection (2018)
  • Author(s): 高倉千裕、王 瀚棠、前田 晃、野口侑記、Lo Pei-Chi、坂井理恵子、松浦 玲、児玉 匡、米山知寿、江口 寛、渡辺将人、長嶋比呂志、奥山宏臣、宮川周士
  • Organizer: 第54回日本移植学会
• [Presentation] PAK-2 inhibitor, PQA-18によるマクロファージ誘導異種移植拒絶反応の抑制効果の検討 (2018)
  • Author(s): Lo Pei-Chi、前田 晃、児玉 匡、坂井理恵子、高倉千裕、米山知寿、松浦 玲、野口侑記、上野豪久、江口 寛、松浪勝義、奥山宏臣 、宮川周士
  • Organizer: 第54回日本移植学会
• [Presentation] 異種移植におけるヒトCD200の好中球誘導拒絶反応の抑制効果の検討 (2018)
  • Author(s): 松浦 玲、前田 晃、Lo Pei-Chi、坂井理恵子、高倉千裕、野口侑記、児玉 匡、江口 寛、渡辺将人、長嶋比呂志、宮川周士、奥山宏臣
  • Organizer: 第54回日本移植学会
• [Presentation] ヒトCD31(PECAM1)によるマクロファージ誘導異種移植拒絶反応の抑制 (2018)
  • Author(s): 野口侑記、前田 晃、児玉 匡、松浦 玲、Lo Pei-Chi、坂井理恵子、高倉千裕、米山知寿、高間勇一、上野豪久，渡辺将人、長嶋比呂志、宮川周士、奥山宏臣
  • Organizer: 第54回日本移植学会
• [Presentation] Human CD31 suppress macrophage-mediated xenogeneic rejection (2018)
  • Author(s): Akira Maeda, Wang Han-Tang, Chihiro Takakura, Rieko Sakai, Pei-Chi Lo, Rei Matsuura, Tasuku Kodama, Shohei Hiwatashi, Hiroshi Eguchi, Hiroomi Okuyama, Shuji Miyagawa
  • Organizer: The Transplantation Society
  • Int'l Joint Research
• [Presentation] PQA-18, a novel immunosuppressant, suppresses macrophage differentiation and macrophage-mediated xenogeneic cytotoxicity (2018)
  • Author(s): Pei-Chi Lo, Rieko Sakai, Akira Maeda, Chihiro Takakura, Rei Matsuura, Tasuku Kodama, Shohei Hiwatashi, Hiroshi Eguchi, Hiroomi Okuyama, Shuji Miyagawa
  • Organizer: The Transplantation Society
  • Int'l Joint Research
• [Presentation] Human CD47 suppresses neutrophil-mediated xenogeneic rejection (2018)
  • Author(s): Rieko Sakai, Pei-Chi Lo, Akira Maeda, Chihiro Takakura, Rei Matsuura, Tasuku Kodama, Shohei Hiwatashi, Hiroshi Eguchi, Hiroomi Okuyama, Shuji Miyagawa
  • Organizer: The Transplantation Society
  • Int'l Joint Research
• [Presentation] The strategies for preventing the macrophage-mediated rejection in xenotransplantation (2018)
  • Author(s): Akira Maeda, Lo Pei-chi, Rieko Sakai, Yuki Noguchi, Tasuku Kodama, Rei Matsuura, Tomohisa Yoneyama, Chihiro Takakura, Hiroshi Eguchi, Masahito Watanabe, Hiroshi Nagashima, Hiroomi Okuyama, Shuji Miyagawa
  • Organizer: APITA 2019, Asia Pancreas and islet Transplantation Association
  • Int'l Joint Research / Invited
• [Presentation] ヒトCD31によるマクロファージ誘導異種移植拒絶反応の抑制 (2018)
  • Author(s): 高倉千裕、王瀚棠、前田晃、坂井理恵子、Lo Pei-Chi、松浦玲、児玉匡、樋渡勝平、 江口寛、奥山宏臣、宮川周士
  • Organizer: 第20回日本異種移植研究会
• [Presentation] The effect of human CD31 transfection to porcine cell in neutrophil-mediated xenogeneic rejection (2018)
  • Author(s): Han-Tang WANG, Akira MAEDA, Rieko SAKAI, Pei-Chi LO, Afifah Mohd SHABRI, Patmika JIARAVUTHISAN, Hiroshi EGUCHI,Shuji Miyagawa
  • Organizer: 第20回日本異種移植研究会
• [Presentation] PQA-18, a novel immunosuppressant, suppresses macrophage differentiation and macrophage-mediated xenogeneic cytotoxicity (2018)
  • Author(s): Pei-Chi Lo, Akira Maeda, Rieko Sakai, Chihiro Takakura, Rei Matsuura, Tasuku Kodama, Shohei Hiwatashi, Hiroshi Eguchi, Hiroomi Okuyama, Shuji Miyagawa
  • Organizer: 第20回日本異種移植研究会
• [Presentation] ブタのヒトに対する自然免疫反応および仔ブタの抗ヒト抗体獲得に関する研究 (2018)
  • Author(s): 坂井理恵子、渡邊將人、前田 晃、Lo Pei-Chi、王 瀚棠、江口 寛、井上 亮、長嶋比呂志、奥山宏臣、宮川周士
  • Organizer: 第20回日本異種移植研究会
• [Presentation] 膜型SP-Dによるマクロファージの制御 (2017)
  • Author(s): Patmika Jiaravuthisan、前田 晃、坂井理恵子、王 瀚棠、Afifah Mohd Shabri、松浦 玲、児玉 匡、江口 寛、奥山宏臣、宮川周士
  • Organizer: 第19回日本異種移植研究会
  • Place of Presentation: 京都大学
  • Year and Date: 2017-02-25
• [Presentation] ヒトCD200による好中球誘導異種移植拒絶反応の抑制効果の検討 (2017)
  • Author(s): Afifah Mod Shabri、前田 晃、坂井理恵子、王 瀚棠、Patmika Jiaravuthisan、松浦 玲、児玉 匡、江口 寛、奥山宏臣、宮川周士
  • Organizer: 第19回日本異種移植研究会
  • Place of Presentation: 京都大学
  • Year and Date: 2017-02-25
• [Presentation] ヒトCD31による好中球誘導異種移植拒絶反応の抑制効果の検討 (2017)
  • Author(s): Han-Tang Wang、前田 晃、坂井理恵子、Afifah Mod Shabri、Patmika Jiaravuthisan、松浦 玲1)、児玉 匡、江口 寛、奥山宏臣、宮川周士
  • Organizer: 第19回日本異種移植研究会
  • Place of Presentation: 京都大学
  • Year and Date: 2017-02-25
• [Presentation] CD200-CD200R会合によるマクロファージ誘導異種移植拒絶反応の抑制 (2017)
  • Author(s): 坂井理恵子、Lo Pei-Chi、前田 晃、王 瀚棠、松浦 玲、児玉 匡、高倉千裕、江口 寛、奥山宏臣、宮川周士
  • Organizer: 第53回日本移植学会
• [Presentation] 臨床でのバイオ人工膵島移植の普及をめざして (2017)
  • Author(s): 宮川周士、前田 晃、坂井理恵子、Lo Pei-Chi、高倉千裕、松浦 玲、児玉 匡、上野豪久、後藤昌史、松本慎一、長嶋比呂志、江口 寛、奥山宏臣
  • Organizer: 第53回日本移植学会
• [Presentation] The innate immunological response of pigs against humans (2017)
  • Author(s): Rieko Sakai, Masahito Watanabe, Akira Maeda, Pei-chi Lo, Han-Tang Wang, Rei Matsuura, Tasuku Kodama, Hiroshi Eguchi, Hitomi Matsunari, Ryo Inoue, Hiroshi Nagashima, Hiroomi Okuyama, Shuji Miyagawa
  • Organizer: The 14th International Xenotransplantation Association, in Baltimore
  • Int'l Joint Research
• [Presentation] Suppression of macrophage-mediated cytotoxicity by a membrane-type SP-D (2017)
  • Author(s): Patmika Jiaravuthisan, Akira Maeda, Han-Tang Wang, Rieko Sakai, Afifah Mohd Shabri, Rei Matsuura, Tasuku Kodama, Hiroshi Eguchi, Hiroomi Okuyama, Shuji Miyagawa
  • Organizer: The 14th International Xenotransplantation Association, in Baltimore
  • Int'l Joint Research
• [Presentation] hCD200 suppresses the M0 macrophage-mediated cytotoxicity through binding to CD200R (2017)
  • Author(s): Akira Maeda, Rieko Sakai, Pei-Chi Lo, Han-Tang Wang, Rei Matsuura, Tasuku Kodama, Hiroshi Eguchi, Hiroomi Okuyama, Shuji Miyagawa
  • Organizer: The 14th International Xenotransplantation Association, in Baltimore
  • Int'l Joint Research
• [Presentation] Human CD31 suppresses the neutrophil-mediated xenogeneic rejection (2017)
  • Author(s): Akira Maeda, Han-Tang Wang, Rieko Sakai, Rei Matsuura, Tasuku Kodama, Hiroshi Eguchi, Hiroomi Okuyama, Shuji Miyagawa
  • Organizer: The 14th International Xenotransplantation Association, in Baltimore
  • Int'l Joint Research
• [Presentation] The membrane-type surfactant protein D may provide an effective therapeutic strategy to inhibit macrophage-mediated xenograft rejection (2017)
  • Author(s): Shuji Miyagawa, Patmika Jiaravuthisan, Akira Maeda, Han-Tang Wang, Pei-chi Lo, Rieko Sakai, Afifah Mohd Shabri, Rei Matsuura, Tasuku Kodama, Takehisa Ueno, Hiroshi Eguchi, Hiroomi Okuyama
  • Organizer: CAST 2017 in Cebu
  • Int'l Joint Research
• [Presentation] The immunological response of pigs anti-human cell, included issues such as the production of natural antibodies in newborn (2017)
  • Author(s): Rieko Sakai, Masahito Watanabe, Akira Maeda, Pei-chi Lo, Han-Tang Wang, Rei Matsuura, Tasuku Kodama, Takehisa Ueno, Hiroshi Eguchi, Hitomi Matsunari, Ryo Inoue, Hiroshi Nagashima, Hiroomi Okuyama, Shuji Miyagawa
  • Organizer: CAST 2017 in Cebu
  • Int'l Joint Research
• [Presentation] CD200 suppresses the M0 but not M1 macrophage mediated xenocytotoxicity (2017)
  • Author(s): Rieko Sakai, Pei-Chi Lo, Akira Maeda, Han-Tang Wang, Rei Matsuura, Tasuku Kodama, Takehisa Ueno, Chihiro Takakura, Hiroshi Eguchi, Hiroomi Okuyama, Shuji Miyagawa
  • Organizer: CAST 2017 in Cebu
  • Int'l Joint Research
• [Presentation] The Strategies for Preventing the Macrophage-mediated Rejection in Xenotransplantation (2016)
  • Author(s): Akira Maeda
  • Organizer: Joint Conference 5th KXA Symposium & 12th Seoul Forum on Xenotransplantation
  • Place of Presentation: ソウル(韓国)
  • Year and Date: 2016-11-24
  • Int'l Joint Research / Invited
• [Presentation] ROSA26-targeted knock-in using CRISPR/Cas9 system in pigs (2016)
  • Author(s): Masahito Watanabe, Rieko Sakai, Kazuhiro Umeyama, Shuko Takayanagi, Nakano Kazuaki, Hitomi Matsunari, Masahito Ikawa, Shuji Miyagawa, Hiroshi Nagashima
  • Organizer: JAACT 2016
  • Place of Presentation: 神戸市
  • Year and Date: 2016-11-09
  • Int'l Joint Research
• [Presentation] CD200強制発現によるマクロファージ誘導細胞傷害活性の制御に関する検討 (2016)
  • Author(s): 坂井理恵子、Afifah Mohd Shabri、前田 晃、児玉 匡、松浦 玲、中畠賢吾、山中和明、河井友美、江口 寛、奥山宏臣、宮川周士
  • Organizer: 第52回日本移植学会
  • Place of Presentation: 東京
  • Year and Date: 2016-09-29
• [Presentation] Human DAF suppresses macrophage-mediated xenogeneic cytotoxicity and phagocytosis through the binding of SCR-4 to the inhibitory receptor (2016)
  • Author(s): Rieko Sakai, Akira Maeda, Thuy-Vy Choi, Pei-Chi Lo, Rei Matsuura, Tasuku Kodama, Kazuaki Yamanaka, Kengo Nakahata, Tomomi Kawai, Hiroshi Eguchi, Hiroomi Okuyama, Shuji Miyagawa
  • Organizer: International Complement Workshop
  • Place of Presentation: 金沢市
  • Year and Date: 2016-09-04
  • Int'l Joint Research
• [Presentation] xpression of complement regulatory factors in the rat renal grafts is associated with the progress of acute T-cell mediated rejection (2016)
  • Author(s): Kazuaki Yamanaka, Shuji Miyagawa, Yoichi Kakuta, Shigeaki Nakazawa, Toyofumi Abe, Ryoichi Imamura, Akira Maeda, Hiroomi Okuyama, and Norio Nonomura
  • Organizer: International Complement Workshop
  • Place of Presentation: 金沢市
  • Year and Date: 2016-09-04
  • Int'l Joint Research
• [Presentation] SCR-4 is required for CD55-induced suppression on macrophage-mediated cytotoxicity (2016)
  • Author(s): Akira Maeda, Thuy-vy Choi, Pei-Chi Lo, Rieko Sakai, Mayumi Asada, Kazuaki Yamanaka, Kengo Nakahata, Rei Matsuura, Hiroshi Eguchi, Hiroomi Okuyama, Shuji Miyagawa
  • Organizer: The Transplantation Society
  • Place of Presentation: 香港(中国）
  • Year and Date: 2016-08-20
  • Int'l Joint Research
• [Presentation] Human CD200 suppresses the M0 macrophage-mediated cytotoxicity in xenotransplantation (2016)
  • Author(s): Akira Maeda, Pei-Chi Lo, Thuy-vy Choi, Rieko Sakai, Mayumi Asada, Kazuaki Yamanaka, Kengo Nakahata, Rei Matsuura, Hiroshi Eguchi, Hiroomi Okuyama, Shuji Miyagawa
  • Organizer: The Transplantation Society
  • Place of Presentation: 香港(中国）
  • Year and Date: 2016-08-20
  • Int'l Joint Research
• [Presentation] Depression of complement regulatory factors in rat and human renal grafts is associated with the progress of acute T-cell mediated rejection (2016)
  • Author(s): Kazuaki Yamanaka, Yoichi Kakuta, Shuji Miyagawa, Shigeaki Nakazawa, Toyofumi Abe, Ryoichi Imamura, Akira Maeda, Hiroomi Okuyama, Norio Nonomura
  • Organizer: The Transplantation Society
  • Place of Presentation: 香港(中国)
  • Year and Date: 2016-08-20
  • Int'l Joint Research
• [Book] REMINISCENCES OF XENOTRANSPLANTATION STUDIES (2018)
  • Author(s): Shuji Miyagawa
  • Total Pages: 25
  • Publisher: Nova science publisher
  • ISBN: 9781536139457