Development and application of liver-specific delivery carrier made by self-aggregate
| Project/Area Number |
16K15063
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Integrative animal science
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| Research Institution | National Defense Medical College |
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Principal Investigator |
NAKAMURA Shingo 防衛医科大学校(医学教育部医学科進学課程及び専門課程、動物実験施設、共同利用研究施設、病院並びに防衛, 防衛医学研究センター, 講師 (00505323)
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| Co-Investigator(Kenkyū-buntansha) |
石原 雅之 防衛医科大学校(医学教育部医学科進学課程及び専門課程、動物実験施設、共同利用研究施設、病院並びに防衛, 防衛医学研究センター 医療工学研究部門, 教授 (10508500)
佐藤 正宏 鹿児島大学, 総合科学域総合研究学系, 教授 (30287099)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 生体材料 / タンパク質輸送システム / 肝機能制御 / タンパク質送達システム / マウス肝機能制御 / 実験動物 / ゲノム編集 / 小胞体ストレス応答 / 糖尿病 |
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| Outline of Final Research Achievements |
We have already reported that biomaterials based on low-molecular weight heparin (LHPPs) can be produced as extremely small particles, simply by mixing raw materials. In this study, we attempted to improve the LHPPs by a simple modification method using electrostatic interactions, and introduced intravenously the marker protein into mice hepatocytes. The results confirmed in vivo accumulation of the transferred protein in mice hepatocytes specifically. These materials are anticipated to be a useful tool for manipulating the function of mouse hepatocytes by using protein.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究の最大の特徴は、生体材料の作製方法として一般的である高度な合成技術等を用いずに、原材料の混合という極めて簡便な方法によってin vivoで機能するタンパク質デリバリーキャリアの作製を試みた点にある。そして、基剤としては汎用医薬品を利用している。本研究の成果は、in vivoでのタンパク質導入に関する基礎研究分野のみならず、医療分野等の応用研究での利用にも期待が持てる。
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Report
(4 results)
Research Products
(24 results)
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[Journal Article] Timing of CRISPR/Cas9-related mRNA microinjection after activation as an important factor affecting genome editing efficiency in porcine oocytes2018
Author(s)
Sato M, Maeda K, Koriyama M, Inada E, Saitoh I, Ohtsuka M, Nakamura S, Sakurai, T, Watanabe S, Miyoshi K
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Journal Title
Theriogenology
Volume: 108
Pages: 29-38
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Low-molecular weight heparin protamine complex augmented the potential of adipose-derived stromal cells to ameliorate limb ischemia2016
Author(s)
Satoko Kishimoto, Ken-ichi Inoue, Shingo Nakamura, Hidemi Hattori, Masayuki Ishihara, Masashi Sakuma, Shigeru Toyoda, Hideki Iwaguro, Isao Taguchi, Teruo Inoue, Ken-ichiro Yoshida
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Journal Title
Atherosclerosis
Volume: 249
Pages: 132-139
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] The piggyBac-Based Gene Delivery System Can Confer Successful Production of Cloned Porcine Blastocysts with Multigene Constructs.2016
Author(s)
Sato M, Maeda K, Koriyama M, Inada E, Saitoh I, Miura H, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S, Miyoshi K.
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Journal Title
Int J Mol Sci.
Volume: 17
Issue: 9
Pages: 1424-1424
DOI
NAID
Related Report
Peer Reviewed / Open Access
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