Suparabasin associated with epidermal keratinocyte differentiation and barrier function
Project/Area Number |
16K19715
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Dermatology
|
Research Institution | Hamamatsu University School of Medicine |
Principal Investigator |
|
Project Period (FY) |
2016-04-01 – 2018-03-31
|
Project Status |
Completed (Fiscal Year 2017)
|
Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | スプラバシン / アトピー性皮膚炎 / 三次元培養皮膚 / フィラグリン / レンチウイルス / suprabasin / 角層バリア機能 / 発汗 |
Outline of Final Research Achievements |
We found that the serum levels of SBSN were significantly lower in AD patients than in normal subjects, supporting the decreased production of SBSN in AD. To investigate the function of SBSN for epidermal differentiation, we knocked down SBSN in a 3D epidermal model transduced with lentivirus-mediated small-hairpin SBSN (shSBSN). Although the 3D model treated with shSBSN exhibited no remarkable morphological change in stratum corneum, it showed a marked reduction in keratohyalin granules. In addition, shSBSN significantly down-modulated the mRNA expression levels of differentiation-associated molecules, including filaggrin, filaggrin-2, loricrin, desmoglein-1, desmocollin-1, corneodesmosin and bleomycin hydrolase, while involucrin, claudin-1, or calpain-1 was not affected. Our findings suggest that the decreased SBSN expression induces abnormal epidermal differentiation and may contribute to the epidermal barrier dysfunction in AD.
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Report
(3 results)
Research Products
(1 results)