Reaction mechanism of [FeFe] hydrogenases
| Project/Area Number |
16K21748
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| Research Category |
Fund for the Promotion of Joint International Research (Home-Returning Researcher Development Research)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biophysics
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| Research Institution | Hokkaido University |
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Principal Investigator |
Ogata Hideaki 北海道大学, 低温科学研究所, 特任准教授 (30795935)
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| Co-Investigator(Kenkyū-buntansha) |
Higuchi Yoshiki 兵庫県立大学, 生命理学研究科, 教授 (90183574)
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| Project Period (FY) |
2017 – 2019
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥56,290,000 (Direct Cost: ¥43,300,000、Indirect Cost: ¥12,990,000)
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| Keywords | 酵素 / ヒドロゲナーゼ / 反応機構 / フーリエ変換赤外分光 / 電気化学 / 金属酵素 / 生物無機化学 / タンパク質 / 分光学 / 構造生物学 / 酵素反応 / 水素 / X線結晶解析 |
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| Outline of Final Research Achievements |
[FeFe] hydrogenases (an electron-bifurcating and a sensory types) have successfully been maturated with the chemically synthesized di-iron complexes. The catalytic activity of the electron-bifurcating [FeFe] hydrogenase showed the high catalytic activity similar to that of the standard [FeFe] hydrogneases. The results of the electron-bifurcating assay with ferredoxin and NADH suggested that one of the iron-sulfur clusters might be the electron bifurcating site. The sensory [FeFe] hydrogenase showed less activity than that of the standard hydrogenase enzyme. The electrochemical FTIR spectroscopic data indicated that the three distinct oxidation states could be assigned.
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| Academic Significance and Societal Importance of the Research Achievements |
電子伝達体フェレドキシンとNADHを用いた電子伝達分岐反応から、HydAサブユニット中の鉄硫黄クラスターが分岐を制御していると提唱した。調節型[FeFe]ヒドロゲナーゼの活性は、触媒型ヒドロゲナーゼの活性能と比べ非常に低いことがわかった。このことから活性中心周辺のアミノ酸の重要性が明らかとなった。本研究で明らかとなった知見はヒドロゲナーゼを模した化学錯体の設計に重要な指針を与える。
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Report
(4 results)
Research Products
(28 results)
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[Journal Article] Nuclear Resonance Vibrational Spectroscopy for Observation of Fe-H/D Bending Modes in Hydrogenases and Nitrogenases2017
Author(s)
C. Pham, H. Wang, N. Mishra, L. Gee, Y. Yoda, T. B. Rauchfuss, V. Pelmenschikov, H. Ogata, E. J. Reijerse, W. Lubitz, N. Khadka, L. Seefeldt, S. P. Cramer
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Journal Title
SPring-8/SACLA Information
Volume: 22
Pages: 104-109
Related Report
Int'l Joint Research
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