Analysis on the spatio-temporal regulation of cellular oncogenesis

• Project/Area Number: 17012014
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Kyoto University (2006-2009); Osaka University (2005)
• Principal Investigator: MATSUDA Michiyuki Kyoto University, 大学院・生命科学研究科, 教授 (10199812)
• Co-Investigator(Kenkyū-buntansha): NAKAMURA Takeshi 東京理科大学, 生命科学研究所, 教授 (60362604) ; KIYOKAWA Etsuko 京都大学, 大学院・医学研究科, 講師 (80300929) ; 黒川 量雄 (独)理化学研究所, 中野生体膜研究室, 研究員 (40333504) ; 福原 茂朋 国立循環器病センター研究所, 循環器形態部, 室長 (70332880)
• Project Period (FY): 2005 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥305,000,000 (Direct Cost: ¥305,000,000); Fiscal Year 2009: ¥62,500,000 (Direct Cost: ¥62,500,000); Fiscal Year 2008: ¥62,500,000 (Direct Cost: ¥62,500,000); Fiscal Year 2007: ¥62,500,000 (Direct Cost: ¥62,500,000); Fiscal Year 2006: ¥62,500,000 (Direct Cost: ¥62,500,000); Fiscal Year 2005: ¥55,000,000 (Direct Cost: ¥55,000,000)
• Keywords: がん / FRETバイオセンサー / 蛍光イメージング / システム生物学 / 細胞増殖因子 / がん遺伝子 / 上皮細胞増殖因子 / Ras / MAPキナーゼ / Shoc2 / FRET / 細胞増殖 / Rac1 / イノシトールリン脂質 / Asef / G蛋白 / セリンスレオニンキナーゼ / Akt / PI3キナーゼ / Raf / transformation / signal / transduction / simulation / 細胞増殖情報伝達系 / シミュレーション / FRETプローブ / MEK / ERK

Research Abstract

We aimed at understanding the oncogene signal transduction network. For this purpose, we have developed biosensors based on the principle of fluorescence resonance energy transfer (FRET) and developed simulation models. These models will be bases of further improved simulation models, which allow us to draw the global picture of oncogene signal transduction network.

Research Products

• [Journal Article] A genetically encoded Forster resonance energy transfer biosensor for two-photon excitation microscopy (2011)
  • Author(s): Kumagai, Y.; Kamioka, Y.; Yagi, S.; Matsuda, M.; Kiyokawa, E.
  • Journal Title: Analytical Chemistry 413(2) Pages: 192-199
• [Journal Article] Multiple decisive phosphorylation sites for the negative feedback regulation of SOS1 via ERK (2010)
  • Author(s): Kamioka, Y.; Yasuda, S.; Fujita, Y.; Aoki, K.; Matsuda, M.
  • Journal Title: Journal of Biological Chemistry 285(43) Pages: 33540-33548
• [Journal Article] Heterogeneity of phosphatidic acid levels and distribution at the plasma membrane in living cells as visualized by a Foerster resonance energy transfer (FRET) biosensor (2010)
  • Author(s): Nishioka, T.; Frohman, M.A.; Matsuda, M.; Kiyokawa, E.
  • Journal Title: Journal of Biological Chemistry 285(46) Pages: 35979-35987
• [Journal Article] Multiple decisive phosphorylation sites for the negative feedback regulation of SOS1 via ERK. (2010)
  • Author(s): Y.Kamioka, S.Yasuda, Y.Fujita, K.Aoki, M.Matsuda
  • Journal Title: J.Biol.Chem. Volume: 285 Pages: 33540-33548
  • Peer Reviewed
• [Journal Article] The scaffold protein Shoc2/SUR-8 accelerates the interaction of Ras and Raf. (2010)
  • Author(s): R.Matsunaga-Udagawa, Y.Fujita, S.Yoshiki, K.Terai, Y.Kamioka, E.Kiyokawa, K.Yugi, K.Aoki, M.Matsuda.
  • Journal Title: J.Biol.Chem. Volume: 285 Pages: 7818-7826
  • Peer Reviewed
• [Journal Article] Visualization of growth signal transduction cascades in living cells withgenetically encoded probes based on Forster resonance energy transfer (2008)
  • Author(s): K. Aoki, E. Kiyokawa, T. Nakamura, and M. Matsuda
  • Journal Title: Philos.Trans.R.Soc.Lond B Biol.Sci 363 Pages: 2143-2151
  • Peer Reviewed
• [Journal Article] The DHRI domain of DOCK180 binds to SNX5 and regulates cation-independent mannose 6-phosphate receptor transport (2008)
  • Author(s): S. Hara, E. Kiyokawa, S. I. Iemura, T. Natsume, T. Wassmer, P. J. Cullen, H. Hiai, and M. Matsuda
  • Journal Title: Mol. Biol. Cell 19 Pages: 3823-3835
  • Peer Reviewed
• [Journal Article] Phosphorylation and activation of the Racl and Cdc42 GEF Asef in A431 cellsstimulated by EGF (2008)
  • Author(s): R. E. Itoh, E. Kiyokawa, K. Aoki, T. Nishioka, T. Akiyama, and M. Matsuda
  • Journal Title: J.Cell Sci 12 Pages: 2635-2642
  • Peer Reviewed
• [Journal Article] Imaging of Rab5 activity identifies essential regulators for phagosome maturation (2008)
  • Author(s): M. Kitano, M. Nakaya, T. Nakamura, S. Nagata, and M. Matsuda
  • Journal Title: Nature 453 Pages: 241-245
  • Peer Reviewed
• [Journal Article] FRET imaging and in silico simulation : analysis of the signaling network of nerve growth factor-induced neuritogenesis (2008)
  • Author(s): T. Nakamura, K. Aoki, and M. Matsuda
  • Journal Title: Brain Cell Biol 36 Pages: 19-30
  • Peer Reviewed
• [Journal Article] Spatiotemporal regulation of Racl for engulfinent of apoptotic cells (2008)
  • Author(s): M. Nakaya, M. Kitano, M. Matsuda, and S. Nagata
  • Journal Title: Proc.Natl.Acad.Sci. U.S.A 105 Pages: 9198-9203
  • Peer Reviewed
• [Journal Article] Rapid turnover rate of phosphoinositides at the front of migrating MDCK cells (2008)
  • Author(s): T. Nishioka, K. Aoki, K. Hikake, H. Yoshizaki, E. Kiyokawa, and M. Matsuda
  • Journal Title: Mol. Biol. Cell 19 Pages: 4213-4223
  • Peer Reviewed
• [Journal Article] Mutation of Dock5, a member of the guanine exchange factor Dock180 superfamily, in the rupture of lens cataract mouse (2008)
  • Author(s): N. Omi, E. Kiyokawa, M. Matsuda, K. Kinoshita, S. Yamada, K. Yamada, Y. Matsushima, Y. Wang, J. Kawai, M. Suzuki, Y. Hayashizaki, and H. Hiai
  • Journal Title: Exp.Eye Res 86 Pages: 828-834
  • Peer Reviewed
• [Journal Article] Quantification of local morphodynamics and local GTPase activity by edgeevolution tracking (2008)
  • Author(s): Y. Tsukada, K Aoki, T. Nakamura, Y. Sakumura, M. Matsuda, and S. Ishii
  • Journal Title: PLoS. Comput. Biol 4
  • Peer Reviewed
• [Journal Article] An essential role for the SHIP2-dependent negative feedback loop in neuritogenesis of NGF-stimulated PC12 cells. (2007)
  • Author(s): Aoki, K., T. Nakamura, T. Inoue, T. Meyer, and M. Matsuda.
  • Journal Title: J. Cell Biol. 177 Pages: 817-827
  • Peer Reviewed
• [Journal Article] Differential Ras activation between caveolae/raft and non-raft microdomains. (2007)
  • Author(s): Fukano, T., A. Sawano, Y. Ohba, M. Matsuda, and A. Miyawaki.
  • Journal Title: Cell Struct. Funct 32 Pages: 915-915
  • NAID: 130004137516
  • Peer Reviewed
• [Journal Article] Rap1-PDZ-GEF1 interacts with a neurotrophin receptor at late endosomes, leading to sustained activation of Rap1 and ERK and neurite outgrowth. (2007)
  • Author(s): Hisata, S., T. Sakisaka, T, Baba, T, Yamada, K. Aoki, M. Matsuda, and Y. Takai.
  • Journal Title: J. Cell Biol. 178 Pages: 843-860
  • Peer Reviewed
• [Journal Article] R-Ras regulates exocytosis by Rg12/Rlf-mediated activation of RalA on endosomes. (2007)
  • Author(s): Takaya, A., A. Kamio, M. Masuda, N. Mochizuki, H. Sawa, M. Sato, K. Nagashima, A. Mizutani, A. Matsuno, E. Kiyokawa, and M. Matuda.
  • Journal Title: Mol. Biol. Cell 18 Pages: 1850-1860
  • Peer Reviewed
• [Journal Article] Akt-PDK1 Complex Mediates EGF-induced Membrane Protrusion through Ral Activation. (2007)
  • Author(s): Yoshizaki, H., N. Mochizuki, Y. Gotoh, and M. Matsuda.
  • Journal Title: Mol. Biol. Cell 18 Pages: 119-128
  • Peer Reviewed
• [Journal Article] R-Ras regulates exocytosis by Rgl2/Rlf-mediated activation of RalA on endosomes. (2007)
  • Author(s): Takaya, A., Kamio, A., Masuda, M., Mochizuki, N., Sawa, H., Sato, M., Nagashima, K., Mizutani, A., Matsuno, A., Kiyokawa.E., Matsuda.M.
  • Journal Title: Mol.Biol.Cell (In Press)
• [Journal Article] Akt-PDK1 Complex Mediates EGF-induced Membrane Protrusion through Ral Activation. (2007)
  • Author(s): Yoshizaki, H., Mochizuki, N., Gotoh, Y., Matsuda, M.
  • Journal Title: Mol.Biol.Cell 18 Pages: 119-128
• [Journal Article] Dynamics of the RAS/ERK map kinase cascade as monitored by fluorescence probes. (2006)
  • Author(s): Fujioka, A., Terai, K., Itoh, R.E., Aoki, K., Nakamura, T., Kuroda, S., Nishida, E.
  • Journal Title: J.Biol.Chem. 281 Pages: 8917-8926
• [Journal Article] M. GTP hydrolysis by the Rho family GTPase TC10 promotes exocytic vesicle fusion. (2006)
  • Author(s): Kawase, K., Nakamura, T., Takaya, A., Aoki, K., Namikawa, K., Kiyama, H., Inagaki, S., Takemoto, H., Saltiel, A.R., Matsuda, M.
  • Journal Title: Dev.Cell 11 Pages: 411-421
• [Journal Article] luorescence (Forster) resonance energy transfer imaging of oncogene activity in living cells. (2006)
  • Author(s): Kiyokawa, E., Hara, S., Nakamura, T., Matsuda, M.
  • Journal Title: Cancer Sci. 97 Pages: 8-15
• [Journal Article] Analysis of the spatiotemporal activation of rho GTPases using Raichu probes. (2006)
  • Author(s): Nakamura, T., Kurokawa, K., Kiyokawa, E., Matsuda, M.
  • Journal Title: Methods Enzymol. 406 Pages: 315-332
• [Journal Article] The amino-terminal B-Raf-specific region mediates calcium-dependent homo-and hetero-dimerization of Raf (2006)
  • Author(s): Terai, K., Matsuda, M.
  • Journal Title: EMBO J. 25 Pages: 3556-3564
• [Journal Article] The Rho-mDial pathway regulates cell polarity and focal adhesion turnover in migrating cells through mobilizing Apc and c-Src. Mol.Cell Biol. (2006)
  • Author(s): Yamana, N., Arakawa, Y., Nishino, T., Kurokawa, K., Tanji, M., Itoh, R.E.
  • Journal Title: Mol.Cell Biol. 26 Pages: 6844-6858
• [Journal Article] Regulation of RalA GTPase by phosphatidylinositol 3-kinase as visualized by FRET probes. (2006)
  • Author(s): Yoshizaki, H., Aoki, K., Nakamura, T., Matsuda, M.
  • Journal Title: Biochem.Soc.Trans. 34 Pages: 851-854
• [Journal Article] Fluorescence (Forster) resonance energy transfer imaging of oncogene activity in living cells. (2006)
  • Author(s): Kiyokawa, E., Hara, S., Nakamura, T., Matsuda, M.
  • Journal Title: Cancer Sci. 97 Pages: 8-15
  • NAID: 10017103513
• [Journal Article] Dynamics of the RAS/ERK map kinase cascade as monitored by fluorescence probes. (2006)
  • Author(s): Fujioka, A., Terai, K., Itoh, R.E., Aoki, K., Nakamura, T., Kuroda, S., Nishida, E., Matsuda, M.
  • Journal Title: J.Biol.Chem. (In press)
• [Journal Article] Interleukin-1 receptor-associated kinase-1 plays an essential role for Toll-like receptor (TLR)7- and TLR9-mediated interferon-{alpha} induction. (2005)
  • Author(s): Uematsu, S., Sato, S., Yamamoto, M., Hirotani, T., Kato, H., Takeshita, F., Matsuda, M., Coban, C., Ishii, K.J., Kawai, T., Takeuchi, O., Akira, S.
  • Journal Title: J.Exp.Med. 201 Pages: 915-923
• [Journal Article] FRET imaging in nerve growth cones reveals a high level of RhoA activity within the peripheral domain. (2005)
  • Author(s): Nakamura, T., Aoki, K., Matsuda, M.
  • Journal Title: Mol.Brain Res. 139 Pages: 277-287
• [Journal Article] Monitoring spatio-temporal regulation of Ras and Rho GTPase with GFP-based FRET probes. (2005)
  • Author(s): Nakamura, T., Aoki, K., Matsuda, M.
  • Journal Title: Methods 37 Pages: 146-153
• [Journal Article] Localized RhoA Activation as a Requirement for the Induction of Membrane Ruffling. (2005)
  • Author(s): Kurokawa, K., Matsuda, M.
  • Journal Title: Mol.Biol.Cell 16 Pages: 4294-4303
• [Journal Article] Mechanism and role of localized activation of Rho-family GTPases in growth factor-stimulated fibroblasts and neuronal cells. (2005)
  • Author(s): Kurokawa, K., Nakamura, T., Aoki, K., Matsuda, M.
  • Journal Title: Biochem.Soc.Trans. 33 Pages: 631-634
• [Journal Article] Involvement of the c-Src-Crk-C3G-Rap1 signaling in the nectin-induced activation of Cdc42 and formation of adherens junctions. (2005)
  • Author(s): Fukuyama, T., Ogita, H., Kawakatsu, T., Fukuhara, T., Yamada, T., Sato, T., Shimizu, K., Nakamura, T., Matsuda, M., Takai, Y.
  • Journal Title: J.Biol.Chem. 280 Pages: 815-825
• [Journal Article] Ras binding opens c-Raf to expose the docking site for MEK. (2005)
  • Author(s): Terai, K., Matsuda, M.
  • Journal Title: EMBO Rep. 6 Pages: 251-255
• [Journal Article] A FRET-based probe for epidermal growth factor receptor bound non-covalently to a pair of synthetic amphipathic helixes. (2005)
  • Author(s): Itoh, R.E., Kurokawa, K., Fujioka, A., Sharma, A., Mayer, B.J., Matsuda, M.
  • Journal Title: Experimental Cell Research 307 Pages: 142-152
• [Journal Article] Local PIP3 accumulation recruits Vav2 and Vav3 to activate Rac1/Cdc42 and initiate neurite outgrowth in nerve growth factor -stimulated PC12 cells. (2005)
  • Author(s): Aoki, K., Nakamura, T., Fujikawa, K., Matsuda, M.
  • Journal Title: Mol.Biol.Cell 16 Pages: 2207-2217
• [Presentation] 上皮管腔形成におけるRac1活性パターンの可視化と制御 (2011)
  • Author(s): 八木俊輔、松田道行、清川悦子
  • Organizer: 第一回新学術領域研究「蛍光生体イメージ」Vivid Workshop
  • Place of Presentation: 蓼科
  • Year and Date: 2011-03-10
• [Presentation] Regulation of Transcriptomes by Stochastic Change in Rho-family GTPase Activity. (2010)
  • Author(s): Hiroko Yukinaga, Eishu Hirata, Kumiko Ui-Tei, Michiyuki Matsuda
  • Organizer: Amrican society for Cell Biology 50th Annual Meeting
  • Year and Date: 2010-12-15
• [Presentation] FRETイメージングと統計的信号解析に基づく自発的な細胞遊走を制御する分子ネットワークの解析 (2010)
  • Author(s): 国田勝行、青木一洋、松田道行
  • Organizer: 第83回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-07
• [Presentation] Rational design of intramolecular FRET biosensors for monitoring Ser/Thr kinase activity (2010)
  • Author(s): Naoki Komatsu, Kazuhiro Aoki, Michiyuki Matsuda
  • Organizer: APRU Research Symposium
  • Place of Presentation: 京都
  • Year and Date: 2010-11-25
• [Presentation] 管腔構造形成過程における信号伝達分子のFRETによる可視化 (2010)
  • Author(s): 八木俊輔、松田道行、清川悦子
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-10-22
• [Presentation] PC12D細胞における (2010)
  • Author(s): 後藤明弘、中村岳史、星野幹雄、松田道行
  • Organizer: dbcAMP及びNGFによって誘導される神経突起生成のシグナル伝達経路のFRETを用いた解析Neuro 2010(第33回日本神経科学大会・第53回日本神経科学会大会・第20回日本神経回路学会大会合同大会)
  • Place of Presentation: 神戸
  • Year and Date: 2010-09-03
• [Presentation] Development of a FRET-based system monitoring Ser/Thr kinase activity in living cells (2010)
  • Author(s): 小松直樹、青木一洋、松田道行
  • Organizer: 第62回日本細胞生物学会大会
  • Year and Date: 2010-05-21
• [Presentation] Development of a FRET-based system monitoring Ser/Thr kinase activity in living cells (2010)
  • Author(s): Naoki Komatsu, Kazuhiro Aoki, Michiyuki Matsud
  • Organizer: 第62回日本細胞生物学会大会
  • Place of Presentation: 大阪
  • Year and Date: 2010-05-21
• [Presentation] Development of FRET probes for two-photon excitation microscopy (2010)
  • Author(s): 熊谷悠香、清川悦子、松田道行
  • Organizer: 第62回日本細胞生物学会大会
  • Place of Presentation: 大阪
  • Year and Date: 2010-05-19
• [Presentation] Transcriptome Analysis of Glioblastoma Cells Expressing FRET Biosensors for GTPases (2010)
  • Author(s): 幸長弘子、平田英周、程久美子、松田道行
  • Organizer: 第62回日本細胞生物学会大会
  • Place of Presentation: 大阪
• [Presentation] Regulation of Transcriptomes by Stochastic Change in Rho-family GTPase Activity (2010)
  • Author(s): 幸長弘子、平田英周、程久美子、松田道行
  • Organizer: グローバルCOE国際シンポジウム/リトリート2010
  • Place of Presentation: 淡路
• [Presentation] Monitoring of phosphatidic acid distribution in the living cells by FRET probes (2010)
  • Author(s): 岡照子、Michael A.Frohman、清川悦子、松田道行
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
• [Presentation] Quantitative kinetic analysis revealed processive phosphorylation of ERK MAP kinase in mammalian cells (2010)
  • Author(s): Kazuhiro Aoki, Michiyuki Matsuda
  • Organizer: 第33回日本分子生物学会年会・第82回日本生化学会大会合同大会
  • Place of Presentation: 神戸
• [Presentation] Spatial regulation of Phosphoinositide turnover in Migrating MDCK Cells : Quantification by FRET Imaging (2008)
  • Author(s): T. Nishioka, K. Aoki, K. Hikake, H. Yoshizaki, E. Kiyokawa, M. Matsuda
  • Organizer: The American Society for Cell Biology 48th Annual Meeting
  • Place of Presentation: Moscone Center, SanFrancisco
  • Year and Date: 2008-12-13
• [Presentation] The DHR-1 domain of DOCK180 Binds to SNXS and RegulatesCationindependent Mannose 6-phosphatate Receptor transport (2008)
  • Author(s): S. Hara, E. Kiyokawa, S. Lemura, T. Natsume, T. Wassmer, P. Cullen, H. Hiai, M. Matsuda
  • Organizer: The American Society for Cell Biology 48th Annual Meeting
  • Place of Presentation: Moscone Center, San Francisco
  • Year and Date: 2008-12-13
• [Presentation] 低分子量G蛋白による細胞運動と膜輸送の制御 (2008)
  • Author(s): 松田道行
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートピアホテル
  • Year and Date: 2008-12-09
• [Presentation] カルシウム依存性のRasを介したERK活性化機構の解析 (2008)
  • Author(s): 吉木さや香、青木一洋、靖川悦子、松田道行
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会・合同大会
  • Place of Presentation: 神戸ポートピアホテル
  • Year and Date: 2008-12-09
• [Presentation] Ras-MAPKカスケードにおけるネガテイブフィードバック経路の解析 (2008)
  • Author(s): 上岡裕治、安田周平、青木一洋、松田道行
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートピアホテル
  • Year and Date: 2008-12-09
• [Presentation] 管腔形成過程におけるRhoファミリーG蛋白質のFRETイメージング (2008)
  • Author(s): 八木俊輔、清川悦子、松田道行
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートピアホテル
  • Year and Date: 2008-12-09
• [Presentation] FRET Imaging of Rho fainily GTP ase activity during cyst and tubuleformation (2008)
  • Author(s): Sunsuke Yagi, Etsuko Kiyokawa, Michiyuki Matsuda
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートピアホテル
  • Year and Date: 2008-12-09
• [Presentation] cAMP刺激によるPC12D細胞の神経突起伸展過程におけるシグナル分子のFRETを用いた解析 (2008)
  • Author(s): 後藤明弘、中村岳史、松田道行
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートピアホテル
  • Year and Date: 2008-12-09
• [Presentation] 細胞運動時におけるリン脂質とGタンパク質活性の可視化、定量化、数理モデル化 (2008)
  • Author(s): 青木一洋
  • Organizer: Gタンパク質特定領域・膜輸送複合体特定領域合同若手ワークショップ
  • Place of Presentation: 神戸セミナーハウス
  • Year and Date: 2008-12-09
• [Presentation] GFP as a Tool to Decipher, the Signal Transduction Network (2008)
  • Author(s): 松田道行
  • Organizer: 第16回日本血管生物医学会学術大会
  • Place of Presentation: 石川県立音楽堂
  • Year and Date: 2008-12-03
• [Presentation] 増殖シグナルネットワークの生細胞イメージング (2008)
  • Author(s): 松田道行
  • Organizer: 24回京都賞記念ワークショップ
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2008-11-12
• [Presentation] 蛍光タンパクによるがん遺伝子情報伝達系の生細胞イメージング (2008)
  • Author(s): 松田道行
  • Organizer: 第67回日本癌学会総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2008-10-28
• [Presentation] The DHR1 domain of DOCK180 binds to SNX5 and regulatescation-independent mannose 6-phosphatate receptor transport (2008)
  • Author(s): 原重雄、清川悦子、松田道行
  • Organizer: 第67回日本癌学会総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2008-10-28
• [Presentation] FRETプローブを用いたMDCK細胞運動時におけるホスホイノシチド代謝の定量的モデル (2008)
  • Author(s): 西岡照子、青木一洋、樋掛和宏、吉崎尚良、清川悦子、松田道行
  • Organizer: 第60回日本細胞生物学会大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2008-06-29
• [Presentation] Spatiotemporal regulation of Racl and Rab5 during phagocytosis of apoptotic cells (2008)
  • Author(s): Michiyuki Matsuda
  • Organizer: Gordon Research Conferences
  • Place of Presentation: University of New England
  • Year and Date: 2008-06-15
• [Presentation] Spatio-temporal regulation of low-molecular-weight GTPases as revealed by FRET probes (2007)
  • Author(s): Michiyuki Matsuda
  • Organizer: Conference on Dynamics of intracellular Reaction Networks
  • Place of Presentation: Max Planck Institute, Dortmund, Germany.
  • Year and Date: 2007-12-12
• [Presentation] Regulation of exocytosis by Ras-and Rho-family GTPases. Janelia Fram Conference. Fluorescent Proteins & Biological Sensors. (2007)
  • Author(s): Michiyuki Matsuda
  • Organizer: Janelia Fram Conference Fluorescent Proteins & Biological Sensors.
  • Place of Presentation: Janelia Farm Research Campus, Ashburn, U.S.A
  • Year and Date: 2007-10-28
• [Presentation] GTPase sensors (2007)
  • Author(s): Michiyuki Matsuda
  • Organizer: Gordon Research Conference
  • Place of Presentation: Magdalen College, Oxford, UK.
  • Year and Date: 2007-09-16
• [Presentation] Identification of molecular network based on FRET imaging and statistical signal processing in randomly migrating HT1080 cells
  • Author(s): 国田勝行、青木一洋、松田道行
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 東北大学
• [Remarks] ホームページ等
• [Remarks] 研究内容
  • URL: http://www.lif.kyoto-u.ac.jp/labs/fret/project.htm
• [Remarks] 発表論文
  • URL: http://www.lif.kyoto-u.ac.jp/labs/fret/publist.htm
• [Remarks]
  • URL: http://www.Dath1.med.kvoto-u.ac.jp/mm/publisthtm
• [Remarks]
  • URL: http://iisi.path1.med.kyoto-u.ac.jp/mm/index.htm