| Project/Area Number |
17590396
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Kagoshima University |
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Principal Investigator |
YOSHIIE Kiyotaka Kagoshima University, Graduate School of Medical and Dental Sciences, Associate Professor (70174886)
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| Co-Investigator(Kenkyū-buntansha) |
ODA Hiroshi Kagoshima University, Graduate School of Medical and Dental Sciences, Professor (40107868)
前野 伸昭 鹿児島大学, 大学院・医歯学総合研究科, 助手 (20305113)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,210,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥210,000)
Fiscal Year 2007: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2006: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2005: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Bartonella / Cat-scratch disease / angiogenesis / endothelial cell / neutrophil / 細胞走化性 / ネコひっかき病 / バルトネラ / 血管内皮細胞 / 人獣共通感染症 / 迅速培養 |
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| Research Abstract |
We purified a pathological factor produced by Battonella henselae which enhances human vascular endothelial cell growth. The factor was a 14kDa protein with strongly basic isoelctrical point, and seemed a kind of cellular regulatory proteins. Since this protein lost its activity after extraction from electrophoreticgel, we could not confirm the protein structure.
We also developed a new method to incubate B. henselae to promote the bacterial growth. Brucella broth was better than convenient Brain-heart infusion broth as a basic media, and B. henselae increased eataly in Brucella broth overlaid to Brucella agar supplimented with 10% rabbit blood This method improved not only isolation of bacterial products and bacterial cells but also isolation period and rate of B. hanselae from canine blood. We isolated 24 bacteria from street cats in Kagoshima, and comfirmed as B. henselae with type I gene. These isolates has similar biological activities as foloows; enhancement of vascular endothelial cell growth, induction of neutrephil chemetaxis and CCL7 expreagan, enhancement fo CCR21 secretion from lymphatic endothelial cells, and inhibition of spontaneous and induced neutrophil apoptosis. But, SDS-PAGE patterns of these isolates showed slight differences in major proteins, which suggested minor variation of naturally infected B. hensalaeto cats in Kagoshima.
We checked but could not comfirm the angiogenetic activity of conditioned medium of B. henseke in BALB/c mice in vivo. As the specificity of pathogenesis of B. henselae against animal species was unknown, further investigation was required.
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