Molecular approach for analysis of in situ feeding by the marine toxic dinoflagellate Dinophyisis

• Project/Area Number: 17K07886
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Aquatic bioproduction science
• Research Institution: Tohoku University
• Principal Investigator: Nishitani Goh 東北大学, 農学研究科, 准教授 (70450781)
• Project Period (FY): 2017-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 貝毒 / 渦鞭毛藻 / 餌料解析 / 分子生態 / 遺伝子 / 繊毛虫 / 有毒微細藻類 / 下痢性貝毒 / 餌料生物

Outline of Final Research Achievements

The food organisms of Dinophysis rotundata, Dinophysis acuminata, and Dinophysis mitra were clarified at the species level by genetic analysis. For Noctiluca which is the red tide cause plankton, two kinds of methods of restriction enzyme and blocking primer method were developed, and feed organisms of Noctiluca were clarified. As a result of applying this method to another plankton, it was found that a parasite existed in the cell of Alexandrium that causing paralytic shellfish poison, and the species of the parasite was successfully identified.

Academic Significance and Societal Importance of the Research Achievements

下痢性貝毒原因プランクトンであるディノフィシスの発生時期を予測できれば、事前に養殖二枚貝の出荷を早める（毒化する前に全て出荷する）ことで被害を抑えられる。いったん貝毒が発生してしまうと、その後数週間から数ヶ月間にわたって貝を出荷できなくなるので、発生予測の意義は大きい。また、プランクトンの分野全般においてこのような手法で餌料解析をしている報告例はほとんどなく、本手法がプランクトンの餌料解析におけるスタンダードになれば、その意義は大きいと思われる。

Research Products

• [Journal Article] Molecular approach for analysis of in situ feeding by the dinoflagellate Noctiluca scintillans (2020)
  • Author(s): Goh Nishitani, Masaomi Shiromoto, Waka Sato-Okoshi, Akira Ishikawa
  • Journal Title: Harmful Algae Volume: 99 Pages: 1-7
  • DOI: 10.1016/j.hal.2020.101928
  • Peer Reviewed / Open Access
• [Journal Article] An effective method for detecting prey DNA from marine dinoflagellates belonging to the genera Dinophysis and Phalacroma using a combination of PCR and restriction digestion techniques (2018)
  • Author(s): Goh Nishitani, Yuki Kosaka, Satoshi Nagai, Yoshihito Takano, Young-Ok Kim, and Akira Ishikawa
  • Journal Title: Plankton and Benthos Research Volume: -
  • Peer Reviewed / Int'l Joint Research
• [Presentation] 赤潮原因種である海産渦鞭毛藻 Noctiluca scintillans の日 本・韓国沿岸域における遺伝的多様性 (2020)
  • Author(s): 城本将臣・石川輝・Young-Ok Kim・北辻さほ・高坂祐樹・田中雅幸・山﨑康裕・長副聡・山本圭吾・大越和加・西谷豪
  • Organizer: 2020年度日本ベントス学会・日本プランクトン学会合同大会
• [Presentation] 日本および韓国沿岸域における赤潮プランクトン夜光虫の遺伝的多様性に関する研究 (2020)
  • Author(s): 城本将臣・石川輝・Young-Ok Kim・北辻さほ・高坂祐樹・田中雅幸・山﨑康裕・長副聡・山本圭吾・大越和加・西谷豪
  • Organizer: 令和2年度日本水産学会東北支部大会
• [Presentation] マガキ幼生の体内で増殖する新規海産プランクトンに関する初報告 (2020)
  • Author(s): 西谷豪
  • Organizer: 令和2年度日本水産学会東北支部大会
• [Presentation] 麻痺性貝毒原因藻Alexandrium tamarense に寄生するAmoebophrya 属に関する研究 (2020)
  • Author(s): 西谷 豪・山本圭吾・中嶋昌紀・柴田佳紀・大越和加・山口峰生
  • Organizer: 令和2年度赤潮・貝毒部会
• [Presentation] Genetic diversity of the red tide-forming dinoflagellate, Noctiluca scintillans, in Japanese and Korean coastal waters (2019)
  • Author(s): Masaomi Shiromoto, Akira Ishikawa, Young-OK Kim, Saho Kitatsuji, Yuki Kosaka, Masayuki Tanaka, Yasuhiro Yamasaki, Waka Sato-Okoshi, Goh Nishitani
  • Organizer: 4the Asian Marine Biology Symposium
  • Int'l Joint Research
• [Presentation] 日本および韓国沿岸域から単離した夜光虫の遺伝的多様性 (2019)
  • Author(s): 西谷豪、城本将臣、Young-OK Kim、北辻さほ、田中雅幸、山﨑康裕、大越和加、石川輝
  • Organizer: 日本プランクトン学会日本ベントス学会合同大会
• [Presentation] 海産従属栄養性渦鞭毛藻Noctiluca scintillansの現場海域における餌料解析方法の開発 (2019)
  • Author(s): 城本将臣・石川 輝・大越和加・西谷 豪
  • Organizer: 平成31年度日本水産学会春季大会
• [Presentation] Development of new primer sets to detect prey DNA from marine dinoflagellate, Dinophysis spp. (2018)
  • Author(s): Goh Nishitani, Natsumi Takagi, Yoshihito Takano, Satoshi Nagai, Young-Ok Kim, Yuki Kosaka, Akira Ishikawa
  • Organizer: 10th EASTHAB Symposium
  • Int'l Joint Research
• [Presentation] An effective method for detecting prey DNA from the genera Dinophysis and Phalacroma by using two specific primer sets (2018)
  • Author(s): Goh Nishitani, Akira Ishikawa
  • Organizer: THE 18TH INTERNATIONAL CONFERENCE ON HARMFUL ALGAE
  • Int'l Joint Research
• [Presentation] A molecular tool for detecting prey items of a heterotrophic marine dinoflagellate, Dinophysis rotundata (2017)
  • Author(s): Goh Nishitani, Natsumi Takagi, Yoshihito Takano, Satoshi Nagai, Young-Ok Kim, and Akira Ishikawa
  • Organizer: The Japanese Society of Fisheries Science International Symposium
  • Int'l Joint Research