Tumor development caused by epigenetic changes in cancer-related genes using organoids as a model
Project/Area Number |
17K15003
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Tumor biology
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Research Institution | National Cancer Center Japan |
Principal Investigator |
Naruse Mie 国立研究開発法人国立がん研究センター, 研究所, 研究員 (80549923)
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Project Period (FY) |
2017-04-01 – 2020-03-31
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Project Status |
Completed (Fiscal Year 2019)
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Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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Keywords | オルガノイド / エピゲノム / エピジェネティクス / 発がん / メチル化 / がん |
Outline of Final Research Achievements |
In this study, we demonstrated that DNA methylation status for imprinted DMRs were maintained in organoids established from mice colon. In cancer research, it has been noted that epigenetic changes occurred in cancer cells play important roles in acquisition of anticancer drug resistance. Therefore, we demonstrated that organoids are also useful as models for cancer epigenome research. In addition, whole-genome bisulfite sequencing analyses enabled us to identify epigenetic changes during the carcinogenic process in vitro.
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Academic Significance and Societal Importance of the Research Achievements |
オルガノイドの片親性発現遺伝子の DMR を詳細に解析した報告はこれまでになく、オルガノイドが組織のエピゲノム の状態を厳密に維持したモデルであることを初めて示した。がん研究においてがん患者の全ゲノムシーケンスは多岐にわたり行われているが、結果には症例毎の違い、がん細胞の不均一性も含まれ、がん研究におけるモデルとしては扱いが困難な点がある。本研究では発がん過程のエピゲノム解析を、生体内に近く且つ簡便化したモデルとして行い、ヒトのがん研究に生かすため、父母由来のアレルを区別可能なマウス正常上皮から樹立し、エピゲノムの変化を感度良く検出可能な点が有用である。
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Report
(4 results)
Research Products
(1 results)