Effects of anesthtetics on orexin system

• Project/Area Number: 17K16739
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Anesthesiology
• Research Institution: University of Miyazaki
• Principal Investigator: SUE NOBUTAKA 宮崎大学, 医学部, 研究員 (80625509)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2019: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2017: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: オレキシン / 吸入麻酔薬 / 静脈麻酔薬 / 視床下部 / 腹側被蓋野

Outline of Final Research Achievements

Intravenous (iv) injection of propofol and inhalational anesthesia sevoflurane dose-dependently inhibited the single unit activity (SUA) of the OX cell in the perifornical hypothalamus (PFH) and lateral hypothalamus (LH). These inhibitory effect of propofol and sevoflurane were blocked by GABA-receptor antagonist, gabazine. NMDA antagonist, ketamine and α2 receptor antagonist, dexmedetomidine did not affect OX cell activity in the LH and PFH.

Academic Significance and Societal Importance of the Research Achievements

臨床で頻用されているプロポフォール（Pro）とセボフルラン(SEV)がGABA受容体を介してオレキシン細胞活動を抑制した。覚醒機構と関連するOX細胞を麻酔薬が抑制することが明らかになった。麻酔覚醒の機序や睡眠と麻酔の違いに関する機構の解明につながる可能性がある。