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Functional significance of the nine-ness of ciliary and basal body microtubules

Research Project

Project/Area Number 18H02475
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 44040:Morphology and anatomical structure-related
Research InstitutionHosei University

Principal Investigator

Hirono Masafumi  法政大学, 生命科学部, 教授 (10212177)

Co-Investigator(Kenkyū-buntansha) 曽和 義幸  法政大学, 生命科学部, 教授 (10519440)
西川 正俊  法政大学, 生命科学部, 准教授 (30444516)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2020: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2018: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
Keywords中心子 / カートホイール / トリプレット微小管 / ダブレット微小管 / 繊毛軸糸 / 周辺微小管 / クラミドモナス / 基底小体 / 超解像蛍光顕微鏡 / 微小管 / 超解像顕微鏡
Outline of Final Research Achievements

Cilia and basal bodies have a conserved structure consisting of nine doublet/triplet microtubules. To investigate the functional significance of this structural pattern, we analyzed the motility of cilia with an abnormal number of microtubules using genetically engineered Chlamydomonas cells. In the process of producing the cells, we revealed that Bld10p, a conserved centriolar protein, plays a central role in the mechanism that determines the number of microtubules. Furthermore, we obtained results suggesting that cilia with 10 microtubules show almost no motility.

Academic Significance and Societal Importance of the Research Achievements

9本の微小管からなる繊毛・基底小体の構造は、ほとんどの真核生物に共通する普遍的構造パターンである。この構造の構築機構は細胞生物学分野において大きな謎であったが、本研究によってその理解を大きく進めることができた。さらに、微小管の数が9本とは異なる繊毛の運動性についてはこれまでまったく知られていなかったが、10本微小管の繊毛の運動性について初めて知見を得ることができた。

Report

(4 results)
  • 2021 Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (34 results)

All 2021 2020 2019 2018

All Journal Article (6 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 6 results,  Open Access: 3 results) Presentation (28 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results)

  • [Journal Article] Distinct chemotactic behavior in the original Escherichia coli K-12 depending on forward-and-backward swimming, not on run-tumble movements2020

    • Author(s)
      Kinosita Yoshiaki、Ishida Tsubasa、Yoshida Myu、Ito Rie、Morimoto Yusuke V.、Goto Kazuki、Berry Richard M.、Nishizaka Takayuki、Sowa Yoshiyuki
    • Journal Title

      Scientific Reports

      Volume: 10 Issue: 1 Pages: 15887-15887

    • DOI

      10.1038/s41598-020-72429-1

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Coupling Ion Specificity of the Flagellar Stator Proteins MotA1/MotB1 of Paenibacillus sp. TCA202020

    • Author(s)
      Onoe Sakura、Yoshida Myu、Terahara Naoya、Sowa Yoshiyuki
    • Journal Title

      Biomolecules

      Volume: 10 Issue: 7 Pages: 1078-1078

    • DOI

      10.3390/biom10071078

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The roles of a flagellar HSP40 ensuring rhythmic beating2019

    • Author(s)
      Zhu X, Poghosyan E., Rezabkova L., Mehall B., Sakakibara H., Hirono M., Kamiya R., Ishikawa T., Yang P.
    • Journal Title

      Molecular Biology of the Cell

      Volume: 30 Issue: 2 Pages: 228-241

    • DOI

      10.1091/mbc.e18-01-0047

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Sodium-powered stators of the bacterial flagellar motor can generate torque in the presence of phenamil with mutations near the peptidoglycan-binding region2019

    • Author(s)
      Ishida T, Ito R, Clark J, Matzke NJ, Sowa Y, Baker MAB
    • Journal Title

      Molecular Microbiology

      Volume: 未定 Issue: 6 Pages: 1689-1699

    • DOI

      10.1111/mmi.14246

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Aurora-A breaks symmetry in contractile actomyosin networks independently of its role in centrosome maturation2019

    • Author(s)
      P. Zhao, X. Teng, S. N. Tantirimudalige, M. Nishikawa, T. Wohland, Y. Toyama, F. Motegi
    • Journal Title

      Developmental Cell

      Volume: 48 Issue: 5 Pages: 631-645

    • DOI

      10.1016/j.devcel.2019.02.012

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] DNA-binding protein H-NS is required for gene silencing and stiffened nucleoprotein filament formation2018

    • Author(s)
      Yuki Yamanaka, Ricksen S Winardhi, Erika Yamauchi, So-ichiro Nishiyama, Yoshiyuki Sowa , Jie Yan, Ikuro Kawagishi, Akira Ishihama, Kaneyoshi Yamamoto
    • Journal Title

      J. Biol. Chem.

      Volume: 293 Issue: 24 Pages: 9496-9505

    • DOI

      10.1074/jbc.ra117.001425

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Bld10p determines the centriole structure independently of the cartwheel2021

    • Author(s)
      A. Noga, T. Ishikawa, M. Hirono
    • Organizer
      19th International Conference on the Cell and Molecular Biology of Chlamydomonas
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Outer and inner arm dynein arrangement at the proximal region of the axoneme revealed by cryo-ET with a phase plate2021

    • Author(s)
      T. Ishikawa, A. Noga, M. Hirono
    • Organizer
      Dynein 2021 International Workshop
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] A suppressor mutation of a Chlamydomonas cilia-less mutant suggests a novel role of Bld10p, an essential protein for centriole assembly2021

    • Author(s)
      Y. Nakazawa, M. Hirono
    • Organizer
      第59回日本生物物理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Bacterial flagellar rotation at low load2020

    • Author(s)
      Y. Sowa, T. Ishida
    • Organizer
      第58回日本生物物理学会
    • Related Report
      2020 Annual Research Report
  • [Presentation] FliL assists flagellar motor rotation in Escherichia coli under low load condition2020

    • Author(s)
      T. Ishida, M. Yoshida, T. Minamino, Y. Sowa
    • Organizer
      第58回日本生物物理学会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Low cooperativity of flagellar motor switching in Vibrio cholerae the bacterium of a single polar flagellum2020

    • Author(s)
      H. Tajima, M. Nishikawa, Y. Miura, Y. Sowa, I. Kawagishi
    • Organizer
      第58回日本生物物理学会
    • Related Report
      2020 Annual Research Report
  • [Presentation] FliL works as a speed regulator of the Escherichia coli flagellar motor at low load2020

    • Author(s)
      Ishida T, Yoshida M, Minamino T, Sowa Y.
    • Organizer
      The 1st International Symposium on Molecular Engine
    • Related Report
      2019 Annual Research Report
  • [Presentation] Cartwheel-independent mechanism for determining the centriolar microtubule number2019

    • Author(s)
      Akira Noga, Mao Horii, and Masafumi Hirono
    • Organizer
      第71回日本細胞生物学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 繊毛の微小管数を9本に決定する2つの機構2019

    • Author(s)
      苗加彰, 堀井麻央, 廣野雅文
    • Organizer
      第52回日本原生生物学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 極低負荷条件下においても大腸菌べん毛モーター回転速度は固定子ユニット数に依存する2019

    • Author(s)
      石田翼,吉多美祐,南野徹,曽和義幸
    • Organizer
      第16回 21世紀大腸菌研究会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Load-dependent speed regulation of the bacterial flagellar motor2019

    • Author(s)
      Ishida T, Yoshida M, Minamino T, Sowa Y.
    • Organizer
      第57回日本生物物理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Analysis of the bacterial flagellar switch using mutant rotor components2019

    • Author(s)
      Kato M, Ishida T, Yoshida M, Sowa Y.
    • Organizer
      第57回日本生物物理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Analysis of shaft-bearing interactions that support the smooth rotation of bacterial flagellar motors2019

    • Author(s)
      Kumazaki Y, Ishida T, Yoshida M, Sowa Y.
    • Organizer
      第57回日本生物物理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Controlling the rotation speed of the bacterial flagellar motor with light-driven rhodopsin2019

    • Author(s)
      Hasegawa S, Abe-Yoshizumi R, Inoue K, Kandori H, Sowa Y.
    • Organizer
      第57回日本生物物理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Ion specificity of chimeric stator proteins between Paenibacillus sp. TCA20 MotB1 and Escherichia coli MotB2019

    • Author(s)
      Onoe S, Yoshida M, Ito M, Sowa Y.
    • Organizer
      第57回日本生物物理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Mechanical symmetry breaking in C. elegans dorsal-ventral axis establishment2019

    • Author(s)
      Masatoshi Nishikawa
    • Organizer
      第57回日本生物物理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Chemoreceptor clustering of Escherichia coli in lateral regions of the cytoplasmic membrane2019

    • Author(s)
      Nana Ito, Masatoshi Nishikawa, Yoshiyuki Sowa, Ikuro Kawagishi
    • Organizer
      第57回日本生物物理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 負荷に依存した大腸菌べん毛モーター回転速度の調節因子2019

    • Author(s)
      石田翼,吉多美祐,南野徹,曽和義幸
    • Organizer
      第18回 微生物研究会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Mechanics of the bacterial flagellar motor in vivo2018

    • Author(s)
      石田翼,笠井大司,蔡栄叔,曽和義幸
    • Organizer
      第56回日本生物物理学会年会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 新規クラミドモナス突然変異株pma2の細胞巨大化と鞭毛数異常2018

    • Author(s)
      三輪彩,原田美咲,内藤紗花,廣野雅文
    • Organizer
      第51回日本原生生物学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Cellular localization of SAS6-L, a paralog of a flagellar basal body protein that self-assembles into a 9-fold symmetrical structure2018

    • Author(s)
      中澤友紀、長尾真仁、苗加彰、Manuel Hilbert、Michel O. Steinmetz、廣野雅文
    • Organizer
      第56回日本生物物理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 「9」を基本とする鞭毛軸糸構造のカートホイール非依存的な決定機構2018

    • Author(s)
      苗加彰、堀井麻央、廣野雅文
    • Organizer
      第89回日本動物学会札幌大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] コレラ菌タウリン走性受容体Mlp37の温度依存的遺伝子発現2018

    • Author(s)
      西山宗一郎,小野木汐里,曽和義幸,浦上弘,川岸郁朗
    • Organizer
      第56回日本生物物理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 細菌べん毛モーターの回転速度と構成ユニット数の関係2018

    • Author(s)
      石田翼,吉多美祐,南野徹,曽和義幸
    • Organizer
      第56回日本生物物理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 光ピンセットを用いた細菌べん毛モーター回転計測系の確立2018

    • Author(s)
      飯島悠太,笠井大司,長谷川爽,曽和義幸
    • Organizer
      第56回日本生物物理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] コレラ菌新規アミノ酸走性応答系の同定2018

    • Author(s)
      田島寛隆,川口徹也,山元季実子,曽和義幸,西山宗一郎,川岸郁朗
    • Organizer
      第56回日本生物物理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Paenibacillus sp. TCA20がもつ二価カチオン駆動型べん毛モーター固定子MotA1MotB1の機能解析2018

    • Author(s)
      尾上さくら,吉多美祐,伊藤政博,曽和義幸
    • Organizer
      第56回日本生物物理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 走化性受容体クラスター形成に対するヒスチジンキナーゼとアダプターの影響2018

    • Author(s)
      山崎友也,伊藤 那奈,西川正俊,曽和義幸,川岸郁朗
    • Organizer
      第56回日本生物物理学会年会
    • Related Report
      2018 Annual Research Report

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Published: 2018-04-23   Modified: 2023-01-30  

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