| Project/Area Number |
18K04905
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 28040:Nanobioscience-related
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| Research Institution | Japan Advanced Institute of Science and Technology |
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Principal Investigator |
Biyani Manish 北陸先端科学技術大学院大学, 共創的国際研究推進本部, 特任教授 (00599780)
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| Co-Investigator(Kenkyū-buntansha) |
高村 禅 北陸先端科学技術大学院大学, 先端科学技術研究科, 教授 (20290877)
山下 啓子 北海道大学, 大学病院, 教授 (70332947)
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| Project Period (FY) |
2018-04-01 – 2023-03-31
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| Project Status |
Completed (Fiscal Year 2022)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | Peptide / Peptide aptamer / Voltammetric sensor / Brest Cancer / Cathepsin E / Portable FET / FET sensor / peptide aptamer |
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| Outline of Final Research Achievements |
A silicon-based FET sensor was developed to measure the expression of cathepsin E (CatE). This was achieved by immobilizing a CatE-specific substrate peptide on the FET surface and measuring CatE cleavage using a palm-sized portable FET device (called FETSOR) (Fig. 1 left). The measurement conditions for operating the biosensor were optimized at pH 7.4 and 0.01X PBS. As a result, 2.3 pmol of CatE successfully distinguished the presence or absence of activity to cleave the substrate peptide (Fig. 1 right). The total charge on the peptide at pH 7.4 was +8.51, which decreased to +3.61 after cleavage, as confirmed by a 0.05 V decrease in the measured gate voltage. Thus, the usefulness of the FETSOR-based CatE measurement was successfully demonstrated and evaluated. The project will end with the achievement of developing a portable peptide-based FET sensor for the simple and rapid detection of the breast cancer biomarker cathepsin E.
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| Academic Significance and Societal Importance of the Research Achievements |
FETSORを用いたCatE測定の有用性を実証・評価することに成功した。乳がんバイオマーカーであるカテプシンEを簡便かつ迅速に検出するための携帯型ペプチドベースFETセンサーの開発という成果をもって、本研究課題の完了とする。
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