Development of peptide FET sensor for highly sensitive and simple detection of breast cancer
| Project/Area Number |
18K04905
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 28040:Nanobioscience-related
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| Research Institution | Japan Advanced Institute of Science and Technology |
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Principal Investigator |
Biyani Manish 北陸先端科学技術大学院大学, 共創的国際研究推進本部, 特任教授 (00599780)
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| Co-Investigator(Kenkyū-buntansha) |
高村 禅 北陸先端科学技術大学院大学, 先端科学技術研究科, 教授 (20290877)
山下 啓子 北海道大学, 大学病院, 教授 (70332947)
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| Project Period (FY) |
2018-04-01 – 2023-03-31
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| Project Status |
Completed (Fiscal Year 2022)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | Peptide / Peptide aptamer / Voltammetric sensor / Brest Cancer / Cathepsin E / Portable FET / FET sensor / peptide aptamer |
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| Outline of Final Research Achievements |
A silicon-based FET sensor was developed to measure the expression of cathepsin E (CatE). This was achieved by immobilizing a CatE-specific substrate peptide on the FET surface and measuring CatE cleavage using a palm-sized portable FET device (called FETSOR) (Fig. 1 left). The measurement conditions for operating the biosensor were optimized at pH 7.4 and 0.01X PBS. As a result, 2.3 pmol of CatE successfully distinguished the presence or absence of activity to cleave the substrate peptide (Fig. 1 right). The total charge on the peptide at pH 7.4 was +8.51, which decreased to +3.61 after cleavage, as confirmed by a 0.05 V decrease in the measured gate voltage. Thus, the usefulness of the FETSOR-based CatE measurement was successfully demonstrated and evaluated. The project will end with the achievement of developing a portable peptide-based FET sensor for the simple and rapid detection of the breast cancer biomarker cathepsin E.
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| Academic Significance and Societal Importance of the Research Achievements |
FETSORを用いたCatE測定の有用性を実証・評価することに成功した。乳がんバイオマーカーであるカテプシンEを簡便かつ迅速に検出するための携帯型ペプチドベースFETセンサーの開発という成果をもって、本研究課題の完了とする。
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Report
(6 results)
Research Products
(32 results)
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[Journal Article] Inhibition of HIV-1 Reverse Transcriptase Activity by the Extractsof Indian Plants2020
Author(s)
K. Ishizuka, Y. Tsutsumi, M. Baba, R. Biyani, C.W. Meng, M. Biyani, M. Takagi, J. Jaiswa, B. Sharma, K. Kojima, T. Takita, K. Yasukawa
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Journal Title
J. Biol. Macromol
Volume: 20
Pages: 17-22
Related Report
Peer Reviewed
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