Analysis of neuroprotective actions of astrocytes in the inflammatory tissues during cerebral infarction
| Project/Area Number |
18K14915
|
|---|
| Research Category |
Grant-in-Aid for Early-Career Scientists
|
| Allocation Type | Multi-year Fund |
|---|
| Review Section |
Basic Section 47040:Pharmacology-related
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
Sekiya Hiroshi 東京大学, 大学院医学系研究科(医学部), 特任助教 (40511374)
|
|---|
| Project Period (FY) |
2018-04-01 – 2023-03-31
|
| Project Status |
Completed (Fiscal Year 2022)
|
| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
|---|
| Keywords | イメージング / 脳梗塞 / グリア細胞 / 神経細胞 / ATP / アストロサイト / 大脳皮質 / 視覚野 |
|---|
| Outline of Final Research Achievements |
In cerebral infarction, one of major problems is the progression of neuronal cell death in the region surrounding the infarction site, known as the penumbra, where blood flow is reduced, but the details of the pathophysiology remain unclear. In this study, a thromboembolic model that more faithfully reflects the pathophysiology was performed, and the activities of neurons and glial cells were visualized and analyzed by fluorescence imaging. Furthermore, fluorescence imaging of extracellular ATP, a gliotransmitter, was achieved, suggesting that glial cells may function to protect neurons through suppression of neuronal activity.
|
| Academic Significance and Societal Importance of the Research Achievements |
脳梗塞は、死亡原因の上位に位置し、死に至らなくても重篤な後遺症が残り得る疾患で、社会に対する負荷も重大である。しかし、脳梗塞は、ヒトを対象とした研究には困難があるなどの理由から、病態の理解は十分ではない。本研究で得られた神経細胞およびグリア細胞の活動動態や、シグナル分子であるグリオトランスミッターの細胞外動態などは、脳梗塞後の病態生理のより詳細な理解に重要であり、病態を踏まえた積極的な治療法の実現に有用であることが十分に期待される。
|
Report
(6 results)
Research Products
(8 results)
-
-
-
[Journal Article] Real-time in vivo imaging of extracellular ATP in the brain with a hybrid-type fluorescent sensor2020
Author(s)
Kitajima N, Takikawa K, Sekiya H, Satoh K, Asanuma D, Sakamoto H, Takahashi S, Hanaoka K, Urano Y, Namiki S, Iino M, Hirose K
-
Journal Title
eLife
Volume: 9
Pages: 1-18
DOI
Related Report
Peer Reviewed / Open Access
-
-
[Journal Article] Chemical Landscape for Tissue Clearing Based on Hydrophilic Reagents.2018
Author(s)
Tainaka K, Murakami TC, Susaki EA, Shimizu C, Saito R, Takahashi K, Hayashi-Takagi A, Sekiya H, Arima Y, Nojima S, Ikemura M, Ushiku T, Shimizu Y, Murakami M, Tanaka KF, Iino M, Kasai H, Sasaoka T, Kobayashi K, Miyazono K, Morii E, Isa T, Fukayama M, Kakita A, Ueda HR.
-
Journal Title
Cell Rep.
Volume: 24
Issue: 8
Pages: 2196-2210
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
-
-
