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Development of virus-binding protein-based technologies for concentration, detection and identification of pathogenic viruses in water environment

Research Project

Project/Area Number 19106009
Research Category

Grant-in-Aid for Scientific Research (S)

Allocation TypeSingle-year Grants
Research Field Civil and environmental engineering
Research InstitutionTohoku University

Principal Investigator

OMURA Tatsuo  東北大学, 大学院・工学研究科, 教授 (30111248)

Co-Investigator(Kenkyū-buntansha) KATAYAMA Hiroyuki  東京大学, 大学院・工学系研究科, 准教授 (00302779)
SANO Daisuke  北海道大学, 大学院・工学研究院, 准教授 (80550368)
WATANABE Toru  山形大学, 農学部, 准教授 (10302192)
MASAGO Yoshifumi  東北大学, 大学院・工学研究科, 助教 (50507895)
Co-Investigator(Renkei-kenkyūsha) UEKI Yo  宮城県保健環境センター, 微生物部, 副主任研究員 (60501062)
Project Period (FY) 2007 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥111,280,000 (Direct Cost: ¥85,600,000、Indirect Cost: ¥25,680,000)
Fiscal Year 2011: ¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2010: ¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2009: ¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2008: ¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2007: ¥43,940,000 (Direct Cost: ¥33,800,000、Indirect Cost: ¥10,140,000)
Keywords感染症 / ウイルス / タンパク質 / 酵素 / 検出技術 / 感染性 / 病原ウイルス / ウイルス吸着タンパク質 / アフィニティクロマトグラフィー / VBP固定化 / ブロッキング / EVE法 / 牡蠣 / リパーゼ / 水循環 / 環境材料
Research Abstract

The objective of this project was to develop novel technologies for recovery, detection and identification of pathogenic viruses in water environments by taking advantages of our own approaches including virus-binding protein (VBP)and enzymatic virus elution (EVE)method. As a result, enteric virus-binding protein (EVBP)that can capture diverse enteric viruses was successfully identified, and novel methodologies for virus recovery from environmental matrixes such as river and estuarine sediments were established. Furthermore, a novel methodology for detecting infectious pathogenic viruses in water by using ethidium monoazide was also developed. These achievements allow us to complete the original targets of this project.

Report

(7 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report   Self-evaluation Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (90 results)

All 2011 2010 2009 2008 2007

All Journal Article (34 results) (of which Peer Reviewed: 34 results) Presentation (53 results) Book (3 results)

  • [Journal Article] Adsorption characteristics of an enteric virus-binding protein to norovirus, rotavirus and poliovirus2011

    • Author(s)
      Imai, T., D. Sano, T. Miura, S. Okabe, K. Wada, Y. Masago and T. Omura
    • Journal Title

      BMC Biotechnology

      Volume: 11 Pages: 123-123

    • NAID

      120003762644

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development of an effective method of viral genomic RNA recovery from environmental silty sediments for quantitative molecular detection2011

    • Author(s)
      Miura, T., Y. Masago, D. Sano and T. Omura
    • Journal Title

      Applied and Environmental Microbiology

      Volume: 77(12) Pages: 3975-3981

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Quantification and genotyping of human sapovirus in the Llobregat River catchment2011

    • Author(s)
      Sano, D., U. Perez, S. Guix, R. M. Pinto, T. Miura, S. Okabe and A. Bosch
    • Journal Title

      Spain. Applied and Environmental Microbiology

      Volume: 77(3) Pages: 1111-1114

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Adsorption characteristics of an enteric virus-binding protein to norovirus, rotavirus and poliovirus2011

    • Author(s)
      T.Imai, 他
    • Journal Title

      BMC Biotechnology

      Volume: 11 Issue: 1 Pages: 123-123

    • DOI

      10.1186/1472-6750-11-123

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Quantification and genotyping of human sapoviruses in the Llobregat River catchment, Spain2011

    • Author(s)
      D.Sano, 他
    • Journal Title

      Applied and Environmental Microbiology

      Volume: 77(3) Issue: 3 Pages: 1111-1114

    • DOI

      10.1128/aem.01721-10

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of a real-time RT-PCR assaycombined with ethidium monoazide treatment for RNA viruses and itsapplication to detect viral RNA after heat exposure2011

    • Author(s)
      Kyungju Kim, 他
    • Journal Title

      Water Science and Technology

      Volume: 63 Pages: 502-507

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Detection of oxidative damages on viral capsid protein for evaluating structural integrity and infectivity of human norovirus2010

    • Author(s)
      Sano, D., R. M. Pinto, T. Omura and A. Bosch
    • Journal Title

      Environmental Science and Technology

      Volume: 44(2) Pages: 808-812

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Norovirus-binding proteins recovered from activated sludge microorganisms with an affinity to a noroviral capsid peptide2010

    • Author(s)
      Sano, D., K. Wada, T. Imai, Y. Masago and T. Omura
    • Journal Title

      Journal of Applied Microbiology

      Volume: 109(6) Pages: 1923-1928

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Detection of sapovirus in oysters2010

    • Author(s)
      Ueki, Y., M. Shoji, Y. Okimura, Y. Miyota, Y. Masago, T. Oka, K. Katayama, N. Takeda, M. Noda, T. Miura, D. Sano and T. Omura
    • Journal Title

      Microbiology and Immunology

      Volume: 54 Pages: 483-486

    • NAID

      10029545455

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development of a real-time RT-PCR assay combined with ethidium monoazide treatment for RNA viruses and its application to detect viral RNA after heat exposure.2010

    • Author(s)
      Kim, K., H. Katayama, M. Kitajima, Y. Tohya and S. Ohgaki
    • Journal Title

      Water Science and Technology

      Volume: 63(3) Pages: 502-507

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Chlorine inactivation of human norovirus, murine norovirus and poliovirus in drinking water2010

    • Author(s)
      Kitajima, M, Y. Tohya, K. Matsubara, E. Haramoto, E. Utagawa and H. Katayama
    • Journal Title

      Letters in Applied Microbiology

      Volume: 51(1) Pages: 119-121

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Chlorine inactivation of human norovirus, murine norovirus and poliovirus in drinking water2010

    • Author(s)
      北島正章, 他
    • Journal Title

      Letters in Applied Microbiology

      Volume: 51 Pages: 119-121

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Norovirus-binding proteins recovered from activated sludge microorganisms with an affinity to a noroviral capsid peptide2010

    • Author(s)
      佐野大輔, 他
    • Journal Title

      Journal of Applied Microbiology

      Volume: 109 Pages: 1923-1928

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Detection of Sapovirus in oysters2010

    • Author(s)
      植木洋, 他
    • Journal Title

      Microbiology and Immunology

      Volume: 54 Pages: 483-486

    • NAID

      10029545455

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Detection of oxidative damages on viral capsid protein for evaluating structural integrity and infectivity of human norovirus2010

    • Author(s)
      Sano, D., R. M. Pinto, T. Omura, A. Bosch.
    • Journal Title

      Environmental Science and Technology 44 (2)

      Pages: 808-812

    • Related Report
      2009 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Detection of oxidative damages on viral capsid protein for evaluating structural integrity and infectivity of human norovirus2010

    • Author(s)
      佐野大輔, 大村達夫, 他
    • Journal Title

      Environmental Science and Technology 44

      Pages: 808-812

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enzymatic Virus Elution法を用いた流入下水からのウイルス検出技術の開発2009

    • Author(s)
      村田有紗, 真砂佳史, 三浦尚之, 今井崇博, 大村達夫
    • Journal Title

      環境工学研究論文集

      Volume: 46 Pages: 197-203

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] 培養できない腸管系ウイルス不活化評価を目的とした外殻タンパク質酸化傷害検出手法の開発2009

    • Author(s)
      佐野大輔, R. M. Pinto, 大村達夫, A. Bosch
    • Journal Title

      環境工学研究論文集

      Volume: 46 Pages: 423-428

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Enzymatic Virus Elution法を用いた流入下水からのウイルス検出技術の開発2009

    • Author(s)
      真砂佳史, 大村達夫, 他
    • Journal Title

      環境工学研究論文集 46

      Pages: 197-203

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 培養できない腸管系ウイルス不活化評価を目的とした外殻タンパク質酸化傷害検出手法の開発2009

    • Author(s)
      佐野大輔, 大村達夫, 他
    • Journal Title

      環境工学研究論文集 46

      Pages: 423-428

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 水環境及び感染性胃腸炎患者から得られたノロウイルスカプシドタンパク質遺伝子の多様性及びアミノ酸配列変異の解析2008

    • Author(s)
      今井崇博, 佐野大輔, 真砂佳史, 大村達夫
    • Journal Title

      環境工学研究論文集

      Volume: 45 Pages: 355-360

    • NAID

      130004801683

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Enzymatic Virus Elution法によるカキ中腸腺からのウイルス誘出技術の開発2008

    • Author(s)
      奥村千恵, 真砂佳史, 佐野大輔, 植木洋, 大村達夫
    • Journal Title

      環境工学研究論文集

      Volume: 45 Pages: 179-186

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] New tools for the study and direct surveillance of viral pathogens in water2008

    • Author(s)
      Bosch, A., S. Guix, D. Sano and R. M. Pinto
    • Journal Title

      Current Opinion in Biotechnology

      Volume: 19 Pages: 295-301

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Enzymatic Virus Elution法によるカキ中腸腺からのウイルス誘出技術の開発2008

    • Author(s)
      大村達夫, 真砂佳史, 他
    • Journal Title

      環境工学研究論文集 45

      Pages: 179-186

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 水環境及び感染性胃腸炎患者から得られたノロウイルスカプシドタンパク質遺伝子の多様性及びアミノ酸配列変異の解析2008

    • Author(s)
      大村達夫, 真砂佳史, 他
    • Journal Title

      環境工学研究論文集 45

      Pages: 355-360

    • NAID

      130004801683

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Attenuation of bacteriophage P22 and Staphylococcus aureus on nonporous fomites determined by plate assay and quantitative PCR2008

    • Author(s)
      真砂佳史, 他
    • Journal Title

      Applied and Environmental Microbiology 74

      Pages: 5838-5840

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Persistence of Caliciviruses in artificially contaminated oysters during depuration2007

    • Author(s)
      Ueki, Y., M. Shoji, A. Suto, T. Tanabe, Y. Okimura, Y. Kikuchi, N. Saito, D. Sano, and T. Omura
    • Journal Title

      Applied and Environmental Microbiology

      Volume: 73(17) Pages: 5698-5701

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] 活性汚泥細菌から分離されたノロウイルス吸着タンパク質 (Norovirus-Binding Proteins : NoVBPs)の特性評価2007

    • Author(s)
      和田圭史, 佐野大輔, 今井崇博, 大村達夫
    • Journal Title

      水環境学会誌

      Volume: 30(12) Pages: 731-736

    • NAID

      10020008499

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Detection of human sapovirus in clams2007

    • Author(s)
      Hansman, G. S., T. Oka, R. Okamoto, T. Nishida, M. Noda, D. Sano, Y. Ueki, T. Imai, T. Omura, O. Nishio, H. Kimura and N. Takeda
    • Journal Title

      Japan.Emerging Infectious Diseases

      Volume: 13 Pages: 620-622

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Sapovirus in water2007

    • Author(s)
      Hansman, G. S., D. Sano, Y. Ueki, T. Imai, T. Oka, K. Katayama, N. Takeda and T. Omura
    • Journal Title

      Japan. Emerging Infectious Diseases

      Volume: 13 Pages: 113-135

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Persistence of Caliciviruses in artificially contaminated oysters during depuration2007

    • Author(s)
      Ueki, Y., M. Shoji, A. Suto, T. Tanabe, Y. Okimura, Y. Kikuchi, N. Saito, D. Sano, T. Omura.
    • Journal Title

      Applied and Environmental Microbiology 73 (17)

      Pages: 5698-5701

    • Related Report
      2009 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Persistence of Caliciviruses in Artificially Contaminated Oysters during Depuration2007

    • Author(s)
      大村 達夫, 他
    • Journal Title

      Applied and Environmental Microbiology 73

      Pages: 5698-5701

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 活性汚泥細菌から分離されたノロウイルス吸着タンパク質(Norovirus-Binding Proteins:NoVBPs)の特性評価2007

    • Author(s)
      大村 達夫, 他
    • Journal Title

      水環境学会誌 30

      Pages: 731-736

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Detection of human Sapovirus in clams, Japan.2007

    • Author(s)
      大村 達夫, 他
    • Journal Title

      Emerging Infectious Diseases 13

      Pages: 620-622

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] Oysters accumulating noroviruses as a tool for disease surveillance system2011

    • Author(s)
      Y.Ueki, 他
    • Organizer
      16th International Symposium on Health-Related Water Microbiology
    • Place of Presentation
      ロトルア,ニュージーランド
    • Year and Date
      2011-09-18
    • Related Report
      2011 Annual Research Report
  • [Presentation] Virus binding-protein with an affinity to multiple genotypes of human norovirus2011

    • Author(s)
      T.Imai, 他
    • Organizer
      IUMS2011
    • Place of Presentation
      札幌
    • Year and Date
      2011-09-06
    • Related Report
      2011 Annual Research Report
  • [Presentation] 活性汚泥からのウイルス吸着タンパク質分離方法の新規開発2011

    • Author(s)
      今井崇博, 他
    • Organizer
      第43回日本水環境学会年会
    • Place of Presentation
      札幌
    • Year and Date
      2011-03-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] Oysters accumulating noroviruses as a tool for disease surveillance system.2011

    • Author(s)
      Ueki, Y., Y. Takahashi, M. Abe, Y. Sato, Y. Okimura, Y. Masago and T. Omura.
    • Organizer
      16th International Symposium on Health-Related Water Microbiology
    • Place of Presentation
      Rotorua, New Zealand
    • Related Report
      2011 Final Research Report
  • [Presentation] Virus binding-protein with an affinity to multiple genotypes of human norovirus.2011

    • Author(s)
      Imai, T., D. Sano, T. Miura, S. Okabe, Y. Masago and T. Omura.
    • Organizer
      International Union of Microbiological Societies 2011 Congress
    • Place of Presentation
      Sapporo, Japan
    • Related Report
      2011 Final Research Report
  • [Presentation] ノロウイルスのカキ中腸腺への蓄積および浄化処理による除去・不活化についての検討2010

    • Author(s)
      真砂佳史, 他
    • Organizer
      第58回日本ウイルス学会学術集会
    • Place of Presentation
      徳島
    • Year and Date
      2010-11-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] 酵素を用いたカキからのノロウイルス濃縮法の検討2010

    • Author(s)
      高橋由理, 他
    • Organizer
      第58回日本ウイルス学会学術集会
    • Place of Presentation
      徳島
    • Year and Date
      2010-11-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] Human enteric bacteria that capture norovirus particles with a specific interaction through histo-blood group antigen-like moiety2010

    • Author(s)
      佐野大輔, 他
    • Organizer
      The 2nd COST 929 Symposium
    • Place of Presentation
      Istanbul, Turkey
    • Year and Date
      2010-10-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] Norovirus-binding bacteria : significance in the environmental dissemination of gas troenteritis viruses2010

    • Author(s)
      佐野大輔, 他
    • Organizer
      The 44th Joint Working Conference on Viral Diseases, US-Japan Cooperative Medical Science Program
    • Place of Presentation
      札幌
    • Year and Date
      2010-06-30
    • Related Report
      2010 Annual Research Report
  • [Presentation] Application of quantitative RT-PCR coupled with ethidium monoazide treatment to selectively detect infective enteric viruses2010

    • Author(s)
      片山浩之, 他
    • Organizer
      第42回日本水環境学会年会
    • Place of Presentation
      福岡大学,福岡
    • Year and Date
      2010-03-17
    • Related Report
      2009 Annual Research Report
  • [Presentation] 陸海連環環境が作りだすノロウイルスと人間の関係2010

    • Author(s)
      真砂佳史, 大村達夫
    • Organizer
      日本生態学会第57回大会
    • Place of Presentation
      東京大学,東京
    • Year and Date
      2010-03-17
    • Related Report
      2009 Annual Research Report
  • [Presentation] Enzymatic Virus Elution(EVE)法を用いた養殖カキ中のノロウイルスの定量2010

    • Author(s)
      真砂佳史, 大村達夫, 他
    • Organizer
      第42回日本水環境学会年会
    • Place of Presentation
      福岡大学,福岡
    • Year and Date
      2010-03-15
    • Related Report
      2009 Annual Research Report
  • [Presentation] インフルエンザウイルス(H1N1およびH5N3亜型)の塩素,モノクロラミンおよび紫外線による不活化特性2010

    • Author(s)
      片山浩之, 他
    • Organizer
      第42回日本水環境学会年会
    • Place of Presentation
      福岡大学,福岡
    • Year and Date
      2010-03-15
    • Related Report
      2009 Annual Research Report
  • [Presentation] Human enteric bacteria that capture norovirus particles with a specific interaction through history-blood group antigen-like moiety2010

    • Author(s)
      Sano, D., A. Suenaga, T. Nakagomi, O. Nakagomi and S. Okabe.
    • Organizer
      The 2nd COST 929 Symposium
    • Place of Presentation
      Istanbul, Turkey
    • Related Report
      2011 Final Research Report
  • [Presentation] Accumulation of oxidative damages on rotavirus capsid protein and its relationship with infectivity2010

    • Author(s)
      Sano, D., K. Tojo, T. Nakagomi, O. Nakagomi, R. M. Pinto, A. Bosch and S. Okabe.
    • Organizer
      The 2nd COST 929 Symposium
    • Place of Presentation
      Istanbul, Turkey
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] Norovirus-binding bacteria : significance in the environmental dissemination of gastroenteritis viruses2010

    • Author(s)
      Sano, D.
    • Organizer
      The 44th Joint Working Conference on Viral Diseases, US-Japan Cooperative Medical Science Program
    • Place of Presentation
      Sapporo, Japan.
    • Related Report
      2011 Final Research Report
  • [Presentation] 培養できない腸管系ウイルス不活化評価を目的とした外殻タンパク質酸化傷害検出手法の開発2009

    • Author(s)
      佐野大輔, 大村達夫, 他
    • Organizer
      第46回環境工学研究フォーラム
    • Place of Presentation
      新島学園短期大学,高崎
    • Year and Date
      2009-11-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] Enzymatic Virus Elution法を用いた流入下水からのウイルス検出技術の開発2009

    • Author(s)
      真砂佳史, 大村達夫, 他
    • Organizer
      第46回環境工学研究フォーラム
    • Place of Presentation
      新島学園短期大学,高崎
    • Year and Date
      2009-11-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] Health risk evaluation of Norovirus via oysters for raw consumption.2009

    • Author(s)
      Masago, Y., Y. Ueki and T. Omura
    • Organizer
      6th Netherlands-Japan Workshop on Water Technology
    • Place of Presentation
      Kyoto, Japan.
    • Year and Date
      2009-10-15
    • Related Report
      2011 Final Research Report
  • [Presentation] Health risk evaluation of Norovirus via oysters for raw consumption2009

    • Author(s)
      真砂佳史, 大村達夫, 他
    • Organizer
      6th Netherlands-Japan Workshop on Water Technology
    • Place of Presentation
      京都大学,京都
    • Year and Date
      2009-10-15
    • Related Report
      2009 Annual Research Report
  • [Presentation] Possible seasonality in prevalence of viral gastroenteritis in the Metro-Manila2009

    • Author(s)
      Masago, Y., C. Okumura, T. Miura, Y. Ueki, A. Suzuki, M. Saito, L. T. Sombrero, S. P. Lupisan, H. Oshitani, R. M. Olveda and T. Omura.
    • Organizer
      the Philippines. International Joint Forum on Infectious Diseases
    • Place of Presentation
      Bangkok, Thailand.
    • Year and Date
      2009-09-16
    • Related Report
      2011 Final Research Report
  • [Presentation] Possible seasonality in prevalence of viral gastroenteritis in the Metro-Manila, the Philippines2009

    • Author(s)
      真砂佳史, 大村達夫, 他
    • Organizer
      International Joint Forum on Infectious Diseases
    • Place of Presentation
      Siam City Hotel, Bangkok, Thailand
    • Year and Date
      2009-09-16
    • Related Report
      2009 Annual Research Report
  • [Presentation] 二枚貝養殖水域の微生物学的水質保全2009

    • Author(s)
      真砂佳史, 大村達夫, 他
    • Organizer
      第12回日本水環境学会シンポジウム
    • Place of Presentation
      お茶の水大学,東京
    • Year and Date
      2009-09-15
    • Related Report
      2009 Annual Research Report
  • [Presentation] 酵素を用いた流入下水からのウイルス検出手法におけるウイルス検出効率の検討2009

    • Author(s)
      真砂佳史, 大村達夫, 他
    • Organizer
      平成21年度土木学会第64回年次学術講演会
    • Place of Presentation
      福岡大学, 福岡
    • Year and Date
      2009-09-04
    • Related Report
      2009 Annual Research Report
  • [Presentation] New detection method for enteric viruses in digestive diverticulum of pacific oyster Crassostrea gigas utilizing enzymes for virus elution2009

    • Author(s)
      Masago, Y., C. Okumura, D. Sano, Y. Ueki, T. Omura.
    • Organizer
      15th International Symposium on Health-Related Water Microbiology
    • Place of Presentation
      Naxos, Greece
    • Year and Date
      2009-06-03
    • Related Report
      2009 Self-evaluation Report
  • [Presentation] New detection method for enteric viruses in digestive diverticulum of pacific oyster, Crassostrea gigas utilizing enzymes for virus elution2009

    • Author(s)
      真砂佳史, 大村達夫, 他
    • Organizer
      15th International symposium on health-related water microbiology
    • Place of Presentation
      Naxos Conference Center, Naxos, Greece
    • Year and Date
      2009-06-03
    • Related Report
      2009 Annual Research Report
  • [Presentation] Genetic diversity and evolution in amino acid sequences of Norovirus genogroup II strains in environmental and clinical samples2009

    • Author(s)
      佐野大輔, 真砂佳史, 大村達夫, 他
    • Organizer
      15th International symposium on health-related water microbiology
    • Place of Presentation
      Naxos Conference Center, Naxos, Greece
    • Year and Date
      2009-06-03
    • Related Report
      2009 Annual Research Report
  • [Presentation] Prevalence and genotypes of Sapovirus in wastewater, oysters and gastroenteritis patients in Japan2009

    • Author(s)
      真砂佳史, 大村達夫, 他
    • Organizer
      15th International symposium on health-related water microbiology
    • Place of Presentation
      Naxos Conference Center, Naxos, Greece
    • Year and Date
      2009-06-03
    • Related Report
      2009 Annual Research Report
  • [Presentation] Development of ethidium monoazide(EMA)-RT-PCR for selective detection of enteric viruses2009

    • Author(s)
      片山浩之, 他
    • Organizer
      15th International symposium on health-related water microbiology
    • Place of Presentation
      Naxos Conference Center, Naxos, Greece
    • Year and Date
      2009-06-03
    • Related Report
      2009 Annual Research Report
  • [Presentation] エチジウムモノアザイドPCRを用いたウイルスの選択的な検出法の開発2009

    • Author(s)
      片山浩之, 他
    • Organizer
      第43回日本水環境学会年会
    • Place of Presentation
      山口大学
    • Year and Date
      2009-03-16
    • Related Report
      2008 Annual Research Report
  • [Presentation] 流入下水からのウイルス濃縮手法の比較検討2009

    • Author(s)
      大村達夫, 真砂佳史, 他
    • Organizer
      平成20年度土木学会東北支部技術研究発表会
    • Place of Presentation
      東北工業大学
    • Year and Date
      2009-03-07
    • Related Report
      2008 Annual Research Report
  • [Presentation] Genetic diversity and evolution in amino acid sequences of Nor ovirus genogroup II strains in environmental and stool samples2009

    • Author(s)
      大村達夫, 真砂佳史, 他
    • Organizer
      International Conference on Sustainability Science 2009 (ICSS2009)
    • Place of Presentation
      東京大学
    • Year and Date
      2009-02-06
    • Related Report
      2008 Annual Research Report
  • [Presentation] Genetic diversity and evolution in amino acid sequences of norovirus genogroup II strains in environmental and clinical samples.2009

    • Author(s)
      Imai, T., D. Sano, Y. Ueki, Y. Masago and T. Omura.
    • Organizer
      The 15th Symposium on Health-Related Water Microbiology
    • Place of Presentation
      Naxos, Greece.
    • Related Report
      2011 Final Research Report
  • [Presentation] Detection of oxidative damages on capsid proteins of norovirus : a new approach to evaluate the infectivity of non-culturable virus.2009

    • Author(s)
      Sano, D., R. M. Pinto, T. Omura and A. Bosch.
    • Organizer
      The 15th Symposium on Health-Related Water Microbiology
    • Place of Presentation
      Naxos, Greece.
    • Related Report
      2011 Final Research Report
  • [Presentation] Prevalence and genotypes of Sapovirus in wastewater, oysters and gastroenteritis patients in Japan.2009

    • Author(s)
      Ueki, Y., H. Uemura, Y. Okimura, Y. Masago, T. Miura, T. Omura and K. Miyota.
    • Organizer
      15th International Symposium on Health-Related Water Microbiology
    • Place of Presentation
      Naxos, Greece.
    • Related Report
      2011 Final Research Report
  • [Presentation] New detection method for enteric viruses in digestive diverticulum of pacific oyster, Crassostrea gigas utilizing enzymes for virus elution.2009

    • Author(s)
      Masago, Y., C. Okumura, D. Sano, Y. Ueki and T. Omura.
    • Organizer
      15th International Symposium on Health-Related Water Microbiology
    • Place of Presentation
      Naxos, Greece.
    • Related Report
      2011 Final Research Report
  • [Presentation] Development of ethidium monoazide (EMA)-RT-PCR for selective detection of enteric viruses2009

    • Author(s)
      Kim, K., H. Katayama, M. Kitajima, Y. Tohya and S. Ohgaki.
    • Organizer
      15th International Symposium on Health-Related Water Microbiology
    • Place of Presentation
      Naxos, Greece.
    • Related Report
      2011 Final Research Report
  • [Presentation] Enteric infectious diseases in the Metro-Manila, the Philippines estimated from enteric viruses in water and bivalve shellfish2008

    • Author(s)
      大村達夫, 真砂佳史, 他
    • Organizer
      Asian-African Research Forum on Emerging and Reemerging Infections
    • Place of Presentation
      北海道大学
    • Year and Date
      2008-12-15
    • Related Report
      2008 Annual Research Report
  • [Presentation] 水環境及び感染性胃腸炎患者から得られたノロウイルスカプシドタンパク質遺伝子の多様性及びアミノ酸配列変異の解析2008

    • Author(s)
      大村達夫, 真砂佳史, 他
    • Organizer
      第45回環境工学研究フォーラム
    • Place of Presentation
      大阪工業大学
    • Year and Date
      2008-11-29
    • Related Report
      2008 Annual Research Report
  • [Presentation] Enzymatic Virus Elution法によるカキ中腸腺からのウイルス誘出技術の開発2008

    • Author(s)
      大村達夫, 真砂佳史, 他
    • Organizer
      第45回環境工学研究フォーラム
    • Place of Presentation
      大阪工業大学
    • Year and Date
      2008-11-28
    • Related Report
      2008 Annual Research Report
  • [Presentation] Evaluation of detection methods targeting host-specific Bacteroides spp. as a microbial source tracking marker2008

    • Author(s)
      大村達夫, 真砂佳史, 他
    • Organizer
      The 6th International Symposium on Southeast Asian Water Environment
    • Place of Presentation
      The Jayakarta Bundung Suite Hotel &Spa(Indonesia)
    • Year and Date
      2008-10-30
    • Related Report
      2008 Annual Research Report
  • [Presentation] Enteric viruses in water and bivalve shellfish in Manila, the Philippines2008

    • Author(s)
      大村達夫, 真砂佳史, 他
    • Organizer
      International Symposium on Southeast Asian Water Environment
    • Place of Presentation
      The Jayakarta Bundung Suite Hotel&Spa(Indonesia)
    • Year and Date
      2008-10-30
    • Related Report
      2008 Annual Research Report
  • [Presentation] Detection of bacteria and enteric viruses from river and estuarine sediment2008

    • Author(s)
      大村達夫, 真砂佳史, 他
    • Organizer
      17th Japan/Korea Symposium on Water Environment
    • Place of Presentation
      石巻専修大学
    • Year and Date
      2008-10-07
    • Related Report
      2008 Annual Research Report
  • [Presentation] 環境水中におけるノロウイルス(NoV)の挙動2008

    • Author(s)
      大村達夫, 真砂佳史, 他
    • Organizer
      第11回日本水環境学会シンポジウム
    • Place of Presentation
      関西大学
    • Year and Date
      2008-09-18
    • Related Report
      2008 Annual Research Report
  • [Presentation] ガラスビーズへのウイルス吸着タンパク質(Virus-Binding Protein : VBP)固定化手法の確立2008

    • Author(s)
      大村達夫, 真砂佳史, 他
    • Organizer
      平成20年度土木学会第63回年次学術講演会
    • Place of Presentation
      東北大学
    • Year and Date
      2008-09-12
    • Related Report
      2008 Annual Research Report
  • [Presentation] Enzymatic Virus Elution (EVE)法による牡蠣中腸腺からのウイルス誘出技術の検討2008

    • Author(s)
      大村 達夫, 他
    • Organizer
      第42回日本水環境学会年会
    • Place of Presentation
      名古屋大学
    • Year and Date
      2008-03-21
    • Related Report
      2007 Annual Research Report
  • [Presentation] 酵素を用いた牡蠣中腸腺からのウイルス誘出におけるRNA抽出および定量PCR阻害の定量的評価2008

    • Author(s)
      大村 達夫, 他
    • Organizer
      平成19年度土木学会東北支部技術研究発表会
    • Place of Presentation
      岩手大学
    • Year and Date
      2008-03-08
    • Related Report
      2007 Annual Research Report
  • [Presentation] Enteric infectious diseases in the Metro-Manila, the Philippines estimated from enteric viruses in water and bivalve shellfish.2008

    • Author(s)
      Masago, Y., C. Okumura, Y. Ueki, A. Suzuki, L. Sombrero, S. Lupisan, H. Oshitani, R. Olveda and T. Omura.
    • Organizer
      Asian-African Research Forum on Emerging and Reemerging Infections
    • Place of Presentation
      Sapporo, Japan.
    • Related Report
      2011 Final Research Report
  • [Presentation] Genetically diverse strains of Norovirus genogroup II in water environment2007

    • Author(s)
      大村 達夫, 他
    • Organizer
      2nd IWA-ASPIRE
    • Place of Presentation
      Perth convention & exhibition cssentre, Perth, Australia
    • Year and Date
      2007-10-30
    • Related Report
      2007 Annual Research Report
  • [Presentation] Affinity isolation of Norovirus-binding proteins from activated sludge microorganisms using a surface-exposed part of viral capsid protein.2007

    • Author(s)
      Sano, D., K. Wada, K. Fukushi and T. Omura.
    • Organizer
      14th International symposium on health-related water microbiology.
    • Place of Presentation
      Tokyo, Japan.
    • Year and Date
      2007-09-10
    • Related Report
      2011 Final Research Report
  • [Presentation] Affinity isolation of Norovirus-binding proteins from activated sludge microorganisms using a surface-exposed part of viral capsid protein2007

    • Author(s)
      Sano, D., K. Wada, K. Fukushi, T. Omura.
    • Organizer
      14th International symposium on health-related water microbiology
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      2007-09-10
    • Related Report
      2009 Self-evaluation Report
  • [Presentation] Affinity isolation of Norovirus-binding proteins from activated sludge microorganisms using a surface-exposed part of viral capsid protein2007

    • Author(s)
      大村 達夫, 他
    • Organizer
      14th Iternational symposium on health-related water microbiology
    • Place of Presentation
      東京大学
    • Year and Date
      2007-09-10
    • Related Report
      2007 Annual Research Report
  • [Presentation] Genetically diverse strains of norovirus genogroup II in water environment.2007

    • Author(s)
      Imai, T., D. Sano, Y. Ueki, K. Fukushi and T. Omura.
    • Organizer
      The 2nd International Water Association Asia-Pacific Regional Group Conference & Exhibition.
    • Place of Presentation
      Perth, Australia.
    • Related Report
      2011 Final Research Report
  • [Book] In George V. Kurladze (ed.), Environmental Microbiology Research Trends2008

    • Author(s)
      Sano, D. and T. Omura.
    • Publisher
      Virus-binding proteins in water environments : natural ligands for human viruses
    • Related Report
      2011 Final Research Report
  • [Book] Environmental Microbiology Research Trends2008

    • Author(s)
      大村 達夫, 他
    • Publisher
      Nova Science Publishers Inc.
    • Related Report
      2007 Annual Research Report
  • [Book] Virus-binding proteins in the water environments : natural ligands for human viruses(G. V. Kurladze ed.)2007

    • Author(s)
      Sano, D., T. Omura.
    • Publisher
      Environmental Microbiology Research Trends
    • Related Report
      2009 Self-evaluation Report

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Published: 2007-04-01   Modified: 2016-04-21  

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