Molecular genetical and cytological analyses on S-locus controlled self-incompatibility in Jaapanese pear

• Project/Area Number: 19380020
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Horticulture/Landscape architecture
• Research Institution: Kobe University
• Principal Investigator: TAKASAKI Takeshi Kobe University, 農学研究科, 准教授 (30314511)
• Co-Investigator(Kenkyū-buntansha): NAKANISHI Tetsu 神戸大学, 大学院・農学研究科, 教授 (80031227) ; PARK Pyoyun 神戸大学, 大学院・農学研究科, 教授 (20147094)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥20,020,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥4,620,000); Fiscal Year 2009: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2008: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000); Fiscal Year 2007: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
• Keywords: 自家不和合性 / ニホンナシ / S-Rnase / 花粉側S遺伝子 / F-boxタンパク質 / S-RNase / F-boxタンパク

Research Abstract

Japanese pear exhibits self-incompatibility, which is controlled by the pistil S gene encoding a ribonuclease (S-RNase) and the pollen S gene at the S-locus. In this study, The F-box protein genes that were polymorphic between haplotypes were clustered around S-RNases. Among them, some were good candidates for pollen S possibly, Using the cytological approaches, we revealed S-RNase uptake in compatible and incompatible pollen tube, and the process of incompatible-pollen tube arrest in style.

Research Products

• [Journal Article] S-genotype assignments of localcultivars in Japanese pear 'Senryo', 'Kuroki', and 'Hogyoku'. (2009)
  • Author(s): Okada, K., Castillo, C., Sawamura, Y., Nakanishi, T., Takasaki-Yasuda, T.
  • Journal Title: J. Japan. Soc. Hort. Sci 78(1) Pages: 55-60
  • NAID: 110007044035
  • Peer Reviewed
• [Journal Article] Renumbering the S-RNase alleles of European pears (Pyrus communis L. ) and cloning the S109-RNase allele. (2009)
  • Author(s): Goldway, M., Takasaki-yasuda, T., Sanzol, J., Mota, M., Zisovich, A., Stern, R. A, Sansavini, S.
  • Journal Title: Scientia Horticulturae 119(4) Pages: 417-422
  • Peer Reviewed
• [Journal Article] Characterisation of partial self-compatibility in the European pear cultivar, 'Grand Champion'. (2009)
  • Author(s): Moriya, Y., Okada, K., Yamamota, K., Iwanami, H., Bessho, Nakanishi, T., Takasaki-Yasuda, T.
  • Journal Title: J. Hortic. Sci. Biotech 84(1) Pages: 77-82
  • Peer Reviewed
• [Journal Article] Characterisation of partial self-compatibility in the European pear cultivar, 'Grand Champion'. (2009)
  • Author(s): Moriya, Y.
  • Journal Title: J.Hortic.Sci.Biotech. 第84巻・第1号 Pages: 77-82
  • Peer Reviewed
• [Journal Article] Renumbering the S-RNase alleles of European pears(Pyrus communis L.)and cloning the S109-RNase allele. (2009)
  • Author(s): Goldway, M.
  • Journal Title: Scientia Horticulturae 第119巻・第9号 Pages: 417-422
  • Peer Reviewed
• [Journal Article] S-genotype assignments of local cultivars in Japanese pear 'Senryo', 'Kuroki', and 'Hogyoku'. (2009)
  • Author(s): Okada, K.
  • Journal Title: J.Japan.Soc.Hort.Sci. 第78巻・第1号 Pages: 55-60
  • Peer Reviewed
• [Journal Article] Selection of self-compatible trees by S4sm-haplotype specific marker in Japanese pear. (2008)
  • Author(s): Okada, K., Tonaka, N., Sawamura, Y., Matumoto, T., Takasaki-Yasuda, T.
  • Journal Title: Acta Hort 800 Pages: 401-408
  • Peer Reviewed
• [Journal Article] S-genotype assignment of European pear cultivars using S-RNase specific CAPS marker. (2008)
  • Author(s): Takasaki-Yasuda, T., Moriya, Y., Iwanami, H., Bessh, Nakanishi, T.
  • Journal Title: Acta. Hort 800 Pages: 391-400
• [Journal Article] Deletion of a 236 kb region around S4-RNase in a stylar-part mutant S4sm-haploype of Japanese pear. (2008)
  • Author(s): Okada, K., Tonaka, N., Moriya, Y., Norioka, N., Sawamura, Y., Matumoto, T., Nakanishi, T., Takasaki-Yasuda, T.
  • Journal Title: Plant Mol. Biol 66(4) Pages: 389-400
  • Peer Reviewed
• [Journal Article] Deletion of a 236 kb region around S_4-RNase in a stylar-part mutant S^<sm>_4-haplotype of Japanese pear. (2008)
  • Author(s): Okada, K.
  • Journal Title: Plant Mol. Biol. 第66巻・第4号 Pages: 389-400
  • Peer Reviewed
• [Journal Article] S-genotype assignment of European pear cultivars using S-Rnase specific CAPS marker. (2008)
  • Author(s): Takasaki-Yasuda, T.
  • Journal Title: Acta. Hort. 第800巻 Pages: 391-400
  • Peer Reviewed
• [Journal Article] Selection of self-compatible trees by S^(sm)_4-haplotype specific marker in Japanese pear. (2008)
  • Author(s): Okada, K.
  • Journal Title: Acta. Hort. 第800巻 Pages: 401-408
  • Peer Reviewed
• [Journal Article] Deletion of a 236 kb region around S_4-RNase in a stylar-part mutant S_4^<sm>-haplotype of Japanese pear. (2008)
  • Author(s): Okada, K.,
  • Journal Title: Plant Mol. Biol. 第66巻・第4号 Pages: 389-400
  • Peer Reviewed
• [Journal Article] Purification andcharacterizationof a non-S-RNase and S-RNases from styles of Japanese pear (Pyrus pyrifola). (2007)
  • Author(s): Norioka, S., Oneyama C., Takuma, s., Shinkawa, T., Ishimizu, T., Nakanishi, T., SakiyamaF.
  • Journal Title: Plant Physiol Biochem 45(12) Pages: 878-86
  • Peer Reviewed
• [Journal Article] Development of a CAPS marker system for genotyping European pear cultivars harboring 17 alleles. (2007)
  • Author(s): Moriya, Y., Yamamoto, K., Okada, K., Iwanami, H., Bessho, Nakanishi, T., Takasaki, T.
  • Journal Title: Plant Cell Rep 26(3) Pages: 345-354
  • Peer Reviewed
• [Journal Article] Development of a CAPS marker system for genotyping European pear cultivars harboring 17 alleles. (2007)
  • Author(s): Moriya, Y.,
  • Journal Title: Plant Cell Rep. 第26巻・第3号 Pages: 345-354
  • Peer Reviewed
• [Presentation] ニホンナシS4-RNase周辺BACコンティグの塩基配列解析 (2009)
  • Author(s): 岡田和馬
  • Organizer: 園芸学会平成21年度秋季大会
  • Place of Presentation: 秋田大学(秋田県)
  • Year and Date: 2009-09-28
• [Presentation] ニホンナシ花柱内における不和合花粉管先端部の微細構造の変化 (2009)
  • Author(s): 河野友美
  • Organizer: 園芸学会平成21年度秋季大会
  • Place of Presentation: 秋田大学(秋田県)
  • Year and Date: 2009-09-28
• [Presentation] Ultrastructural observation of the compatible and incompatible pollen tube in the style in S-homozygotes of the Jananese pear. (2009)
  • Author(s): 河野友美
  • Organizer: 6^<th> International Symposium on Electron Microscopy in Medicine and Biology
  • Place of Presentation: 神戸大学(兵庫県)
  • Year and Date: 2009-09-16
• [Presentation] ニホンナシ花柱内における不和合花粉管先端部の微細構造の変化. (2009)
  • Author(s): 河野友美・山北由貴・太田文清・朴杓允中西テツ・安田(高崎)剛志
  • Organizer: 園芸学会2009年度秋季大会
  • Place of Presentation: 秋田大学
• [Presentation] ニホンナシS4-RNase周辺BACコンティグの塩基配列解析. (2009)
  • Author(s): 岡田和馬・戸中希・市川雄彦・潭村豊・中西テツ・安田(高崎)剛志
  • Organizer: 園芸学会2009年度秋季大会
  • Place of Presentation: 秋田大学
• [Presentation] Ultrastructural observation of the compatible and incompatible pollen tube in the style in S-homozygotes of the Japanese pear. (2009)
  • Author(s): Kono, Y., Y. Yamakita, F. Ota, P. Park, T. Nakanishi, T. Takasaki-Yasuda.
  • Organizer: 6th International Symposium on Electron Microscopy in Medicine and Biology
  • Place of Presentation: Japan
• [Presentation] ニホンナシ花柱における自家・他家花粉管の微細構造の解析 (2008)
  • Author(s): 河野友美・山北由貴・太田文清・朴杓允・中西テツ・安田(高崎)剛志
  • Organizer: 園芸学会2008年度秋季大会
  • Place of Presentation: 三重大学
• [Presentation] ニホンナシS2-RNase周辺領域のBACコンティグの塩基配列解析 (2008)
  • Author(s): 田口富雄・戸中希・岡田和罵・中西テツ・安田(高崎)剛志
  • Organizer: 園芸学会2008年度秋季大会
  • Place of Presentation: 三重大学
• [Presentation] ニホンナシ在来品種'千両', '黒木'と'宝玉'のS遺伝子型同定 (2008)
  • Author(s): 岡田和馬・Calros Castillo・潭村豊・中西テツ・安田(高崎)剛志
  • Organizer: 園芸学会2008年度秋季大会
  • Place of Presentation: 三重大学
• [Presentation] ニホンナシS2-RNase周辺領域のBACコンティグの塩基配列解析 (2008)
  • Author(s): 田口富雄
  • Organizer: 園芸学会
  • Place of Presentation: 三重大学
• [Presentation] ニホンナシS2-およびS3-RNase周辺領域のBACコンティグの構築 (2007)
  • Author(s): 戸中希・岡田和罵・潭村豊・中西テツ・安田(高崎)剛志
  • Organizer: 園芸学会2007年度秋季大会
  • Place of Presentation: 香川大学
• [Presentation] ニホンナシS4ハプロタイプにおける236kb欠失領域の塩基配列解析 (2007)
  • Author(s): 岡田和罵・戸中希・潭村豊・中西テツ・安田(高崎)剛志
  • Organizer: 園芸学会2007年度秋季大会
  • Place of Presentation: 香川大学
• [Presentation] セイヨウナシ三倍体品種のS遺伝子型推定 (2007)
  • Author(s): 守谷友紀・山本健太朗・岡田和馬・岩波宏・古籐田信博・中西テツ・安田(高崎)剛志
  • Organizer: 園芸学会平成19年度秋季大会
  • Place of Presentation: 香川大学
• [Presentation] Selection of self-compatible trees by S4sm-haplotype specific marker in Japanese pear. (2007)
  • Author(s): Okada, K., Tonaka, N., Sawamura, Y., Matumoto, T., Takasaki-Yasuda, T.
  • Organizer: 10th International pear symposium
  • Place of Presentation: Portugal
• [Presentation] S-gentype assignment of European pear cultivars using S-RNase specific CAPS marker. (2007)
  • Author(s): akasaki-Yasuda, T. Moriya, Y., Iwanami, H., Bessho, Nakanishi, T.
  • Organizer: 10th International pear symposium
  • Place of Presentation: Portugal
• [Presentation] Selection of self-compatible trees by S_4^<sm>-haplotype specific marker in Japanese pear. (2007)
  • Author(s): Okada, K.,
  • Organizer: 10th international pear symposium
  • Place of Presentation: ホルトガル
• [Presentation] S-genotype assignment of European pear cultivars using S-RNase specific CAPS marker (2007)
  • Author(s): Takasaki-Yasuda, T.
  • Organizer: 10th International pear symposium
  • Place of Presentation: ホルトガル
• [Presentation] ニホンナシS4^<sm>ハプロタイプにおける236kb欠失領域の塩基配列解析 (2007)
  • Author(s): 岡田和馬
  • Organizer: 園芸学会平成19年度秋季大会
  • Place of Presentation: 香川大学
• [Presentation] ニホンナシS2-およびS3-RNase周辺領域のBACコンティグの構築 (2007)
  • Author(s): 戸中希
  • Organizer: 園芸学会平成19年度秋季大会
  • Place of Presentation: 香川大学